prikaz prve stranice dokumenta Molekularna karakterizacija kvazivrsta hrvatskih izolata virusa hepatitisa A
  Download
PDF 2.64 MB
master's thesis
Molekularna karakterizacija kvazivrsta hrvatskih izolata virusa hepatitisa A
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:891678
Kuzle, Josipa
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Kuzle, J. (2020). Molekularna karakterizacija kvazivrsta hrvatskih izolata virusa hepatitisa A (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:891678

Kuzle, Josipa. "Molekularna karakterizacija kvazivrsta hrvatskih izolata virusa hepatitisa A." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:891678

Kuzle, Josipa. "Molekularna karakterizacija kvazivrsta hrvatskih izolata virusa hepatitisa A." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:891678

Kuzle, J. (2020). 'Molekularna karakterizacija kvazivrsta hrvatskih izolata virusa hepatitisa A', Master's thesis, University of Zagreb, Faculty of Science, accessed 28 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:891678

Kuzle J. Molekularna karakterizacija kvazivrsta hrvatskih izolata virusa hepatitisa A [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2025 March 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:891678

J. Kuzle, "Molekularna karakterizacija kvazivrsta hrvatskih izolata virusa hepatitisa A", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:891678

Metadata
TitleMolekularna karakterizacija kvazivrsta hrvatskih izolata virusa hepatitisa A
Title (english)Molecular characterization of Hepatitis A virus quasispecies from Croatian isolates
AuthorJosipa Kuzle
MentorSilvija Černi (mentor)
MentorIvana Grgić (mentor)
Committee memberSilvija Černi (predsjednik povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2020-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Virus hepatitisa A (HAV) jednolančani je RNA virus pozitivnog smisla iz porodice Picornaviridae i glavni uzročnik akutne bolesti jetre u svijetu. Broj zaraženih u Hrvatskoj porastao je u posljednjih nekoliko godina. Kao i ostali virusi s RNA-genomom, HAV ima tendenciju nakupljanja mutacija zbog brze replikacije i nemogućnosti popravaka pogrešaka enzima RNA-polimeraze ovisne o RNA. Zbog toga virusni izolati sadrže velik broj mutanata/genomskih varijanti, koje zajedno tvore tzv. kvazivrste.... More Varijante unutar kvazivrste mogu se razlikovati u svom patogenom potencijalu, no rutinske analize ne uključuju njihovu karakterizaciju. Gotovo nema literaturnih podataka o genskoj raznolikosti varijanti virusa HAV unutar pojedinih izolata te o prirodi prisutnih mutacija. Stoga je cilj ovog rada bio molekularno karakterizirati genomske varijante virusa HAV razdvojene kloniranjem, podrijetlom iz 13 pacijenata. Analizom hipervarijabilne regije genoma VP2-2A svakog uzorka obuhvaćeno je po 20 varijanti, koje su identificirane metodom SSCP i sekvencirane. Usporedbom virusnih mutanata s dominantnom populacijskom varijantom svakog uzorka utvrđena je brojnost i priroda mutacijskih događaja. Rezultati ukazuju na česte mutacijske događaje nasumično pozicionirane unutar analizirane regije. Detektiran je i visok postotak nesinonimnih mutacija, što može imati utjecaj na biološke karakteristike virusa. Kod pacijenata koinficiranih virusom HIV zabilježen je manji broj promjena na nukleotidnoj i aminokiselinskog razini u odnosu na ostale pacijente. Less
Abstract (english)
Hepatitis A virus (HAV) is a positive single-stranded RNA virus from Picornaviridae family. In Croatia number of people infected with HAV increased in recent years. As other RNA viruses, HAV tends to accumulate mutations due to rapid replication and error-prone nature of RNA-dependent RNA polymerase. Consequently, viral isolates contain large number of genomic variants, which together form so-called quasispecies. Different variants within quasispecies may differ in their pathogenic... More potential, but their characterization is not a part of routine analysis. There is almost no literature on quasispecies structure of HAV. The aim of this study was to molecularly characterize genomic variants, previously separated by cloning, from 13 patients. The analysis of VP1-2A hypervariable genomic region included 20 variants per sample, which were identified by SSCP method and sequenced. The number and nature of mutational events were determined by comparing nucleotide and amino-acid sequence of mutants with those of dominant population variant from each sample. The results indicate frequent mutations randomly positioned through the analysed region. High percentage of non-synonymous mutations was detected, that can have a significant impact on the virus biology. In comparison to others, in patients co-infected with HIV fewer nucleotide and amino acid changes was detected. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:891678
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-09-28
Terms of useLicence In copyright icon
Created on2020-10-07 09:05:19
accessibility

closeAccessibilityrefresh

If you wish to permanently save changes, click on Save, if not - your settings will be reset when you restart the browser.