prikaz prve stranice dokumenta Sinteza nano čestica zlata i njihova aplikacija na stanice raka dojke MDA-MB-231
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master's thesis
Sinteza nano čestica zlata i njihova aplikacija na stanice raka dojke MDA-MB-231
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:049026
Hanžić, Nikolina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Hanžić, N. (2013). Sinteza nano čestica zlata i njihova aplikacija na stanice raka dojke MDA-MB-231 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:049026

Hanžić, Nikolina. "Sinteza nano čestica zlata i njihova aplikacija na stanice raka dojke MDA-MB-231." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:049026

Hanžić, Nikolina. "Sinteza nano čestica zlata i njihova aplikacija na stanice raka dojke MDA-MB-231." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:049026

Hanžić, N. (2013). 'Sinteza nano čestica zlata i njihova aplikacija na stanice raka dojke MDA-MB-231', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:049026

Hanžić N. Sinteza nano čestica zlata i njihova aplikacija na stanice raka dojke MDA-MB-231 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:049026

N. Hanžić, "Sinteza nano čestica zlata i njihova aplikacija na stanice raka dojke MDA-MB-231", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:049026

Metadata
TitleSinteza nano čestica zlata i njihova aplikacija na stanice raka dojke MDA-MB-231
Title (english)Synthesis and application of gold nanoparticiples to MDA-MB-231 breast cancer cells
AuthorNikolina Hanžić
MentorMarijan Gotić (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberMarijan Gotić (član povjerenstva)
Committee memberDijana Škorić (član povjerenstva)
Committee memberDubravka Hranilović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-01-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cilj ovog diplomskog rada bio je utvrditi radiosensitizacijski efekt nano čestica zlata (NZ) na stanicama raka dojke MDA-MB-231. Sintetizirane su tri vrste NZ obavijene citratima, glutationom i amino-dekstranom. Njihova veličina određena je skenirajućim elektronskim mikroskopom i UV-Vis-spektrofotometrom i iznosila je oko 15 nm. NZ su bile aplicirane na MDA-MB-231 stanice i pratilo se kvalitativno i kvantitativno nakupljanje NZ u stanicama. Transmisijski elektronski mikroskop je pokazao nakupljanje NZ u vezikulama, a kvantitativnom analizom je utvrđena točna koncentracija NZ u stanicama. Najveća koncentracija NZ u stanicama je postignuta s NZ obavijenim amino-dekstranom. Stanice tretirane NZ bile su izložene γ-zračenju, te se proučavao radiosensitizacijski efekt NZ na MDA-MB-231 stanice. MTT-testom utvrđeno je smanjenje vijabilnosti stanica uslijed aplikacije NZ i γ-zračenja. Klonogenski test pokazao se kao jako osjetljivi test za utvrđivanje utjecaja γ-zračenja + NZ na citotoksičnost stanica. Međutim, zbog velike citotoksičnosti samog γ-zračenja radiosensitizacijski efekt nije mogao biti utvrđen.
Abstract (english)
The aim of this work was to study the radiosensitization effect of three types of gold nanoparticles (GNPs) on MDA-MB-231 cancer cells in vitro. GNPs were synthesized in order to be coated with citrate, glutathione and amino-dextrane. Their size was determined using electron microscopy and UV-VIS spectrophotometer and was around 15 nm. GNPs were applied to a MDA-MB-231 cancer cell line and their accumulation in cells was investigated. Transmission electron microscopy demonstrated accumulation of GNPs in cytoplasmic vesicles, while the Mass Spectroscopy was used to demonstrate the highest uptake of amino- dextrane coated GNPs. Cells treated with GNPs were γ-irradiated and their radiosensitization effect was studied. Short-term MTT viability assay showed no cells sensitivity to the effect of ionizing radiation, whereas the long-term clonogenic assay showed high sensitivity to the cytotoxic effect of γ-irradiation. However, the high cytotoxicity of γ-irradiation alone to cancer cells did not allow the determination of the radiosensitization effect of synthesized GNPs.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:049026
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 08:18:25