prikaz prve stranice dokumenta Identifikacija domena proteina IqgC potrebnih za njegovu regrutaciju na makropinosome u amebi Dictyostelium discoideum
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master's thesis
Identifikacija domena proteina IqgC potrebnih za njegovu regrutaciju na makropinosome u amebi Dictyostelium discoideum
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:744634
Bajt, Petra
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Bajt, P. (2020). Identifikacija domena proteina IqgC potrebnih za njegovu regrutaciju na makropinosome u amebi Dictyostelium discoideum (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:744634

Bajt, Petra. "Identifikacija domena proteina IqgC potrebnih za njegovu regrutaciju na makropinosome u amebi Dictyostelium discoideum." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:744634

Bajt, Petra. "Identifikacija domena proteina IqgC potrebnih za njegovu regrutaciju na makropinosome u amebi Dictyostelium discoideum." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:744634

Bajt, P. (2020). 'Identifikacija domena proteina IqgC potrebnih za njegovu regrutaciju na makropinosome u amebi Dictyostelium discoideum', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:744634

Bajt P. Identifikacija domena proteina IqgC potrebnih za njegovu regrutaciju na makropinosome u amebi Dictyostelium discoideum [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:744634

P. Bajt, "Identifikacija domena proteina IqgC potrebnih za njegovu regrutaciju na makropinosome u amebi Dictyostelium discoideum", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:744634

Metadata
TitleIdentifikacija domena proteina IqgC potrebnih za njegovu regrutaciju na makropinosome u amebi Dictyostelium discoideum
Title (english)Identification of IqgC domains required for its recruitment to macropinosomes in amoeba Dictyostelium discoideum
AuthorPetra Bajt
MentorVedrana Filić Mileta (mentor)
Committee memberAleksandra Maršavelski (predsjednik povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberMorana Dulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2020-10-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Protein IqgC iz amebe Dictyostelium discoideum spada u obitelj proteina IQGAP. Dosadašnja istraživanja su pokazala da IqgC, za razliku od drugih proteina IQGAP, ima sačuvanu GAP (engl. GTPase activating protein) aktivnost odnosno da može stimulirati GTP-hidrolitičku aktivnost male GTPaze RasG. Nadalje, pokazano je da je deaktivacija RasG pomoću IqgC važna za regulaciju unosa tekućine (makropinocitoza) i čestica (fagocitoza) tijekom hranjenja ove amebe te da IqgC lokalizira na membranu endosoma tijekom oba procesa. U ovom radu je ispitan doprinos pojedinih regija/domena proteina IqgC njegovoj regrutaciji na makropinosom. U tu svrhu eksprimirane su tri skraćene varijante proteina koje su sadržavale (1) samo GRD (engl. GAP related domain) domenu, (2) samo RGCt (engl. Ras-GAP-C_terminus) domenu i (3) GRD domenu i N-kraj proteina. Analiza lokalizacije ovih domena u živim stanicama deficijentnim za IqgC upotrebom konfokalne mikroskopije pokazala je da nijedna od njih nije dovoljna za lokalizaciju IqgC na membranu endosoma tijekom makropinocitoze.
Abstract (english)
Dictyostelium discoideum IQGAP-related protein IqgC is an atypical member of IQGAP protein family. Namely, recent study demonstrated that IqgC has conserved GAP (GTPase activating protein) activity and can deactivate Ras protein, while other IQGAP proteins have lost this activity due to mutations in their GRD (GAP related domain). IqgC exerts GAP activity toward small GTPase RasG during liquid uptake or macropinocytosis and particle uptake or phagocytosis, and thus negatively regulates RasG activity during both types of large-scale endocytosis. Moreover, full-length IqgC localizes to both macropinosomes and phagosomes. Using confocal microscopy, we have investigated the subcellular localization of truncated IqgC variants expressed as fluorescent protein fusions in iqgC knock-out cells. We showed that neither of the tested constructs, GRD alone, RGCt (Ras-GAP-C_terminus) domain alone and GRD with adjacent N-terminus, are sufficient for the recruitment of IqgC to endosomal membrane during macropinocytosis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:744634
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-10-28 13:18:12