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master's thesis
Karakterizacija proteina DRG1 iz spužve Eunapius subterraneus
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:552357
Modrić, Matea
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Modrić, M. (2020). Karakterizacija proteina DRG1 iz spužve Eunapius subterraneus (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:552357

Modrić, Matea. "Karakterizacija proteina DRG1 iz spužve Eunapius subterraneus." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:552357

Modrić, Matea. "Karakterizacija proteina DRG1 iz spužve Eunapius subterraneus." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:552357

Modrić, M. (2020). 'Karakterizacija proteina DRG1 iz spužve Eunapius subterraneus', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:552357

Modrić M. Karakterizacija proteina DRG1 iz spužve Eunapius subterraneus [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 May 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:552357

M. Modrić, "Karakterizacija proteina DRG1 iz spužve Eunapius subterraneus", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:552357

Metadata
TitleKarakterizacija proteina DRG1 iz spužve Eunapius subterraneus
Title (english)Characterization of sponge Eunapius subterraneus DRG1 protein
AuthorMatea Modrić
MentorHelena Ćetković (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberNives Galić (član povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberAleksandra Maršavelski (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2020-10-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Spužve, morfološki jednostavne životinje bez tkiva i organa koje se nalaze na samoj bazi filogenetskog stabla Metazoa, predstavljaju dobar model za objašnjenje evolucijskih obrazaca razvoja životinja. Važne su za proučavanje karakteristika homologa kod pretka svih Metazoa prije odvajanja i specijalizacije ovih gena u razvijenijim životinjama i za razumijevanje osnovne fiziološke funkcije gena povezanih s nastankom raka kod jednostavnijih životinja. U okviru diplomskog rada okarakteriziran je protein iz slatkovodne špiljske spužve Eunapius subterraneus čiji je homolog kod čovjeka DRG1 (engl. Developmentally Regulated GTP binding protein 1) povezan s nastankom raka. Cilj je dobiti što bolji uvid u evoluciju ovog supresora metastaziranja. cDNA koja kodira za protein DRG1 uspješno je uklonirana u ekspresijski vektor pET28b te je napravljena prekomjerna ekspresija koja je potvrđena western blotting tehnikom. Protein DRG1 ima GTPaznu aktivnost koja je testirana mjerenjem količine neiskorištenog GTP-a nakon reakcije hidrolize. Osim toga, protein je podvrgnut metodi gel-filtracije kako bi se procijenila njegova molekularna masa.
Abstract (english)
Sponges, morphologically simple animals without tissues and organs and possibly the earliest branching animal phylum, represent a good model for explaining the evolutionary patterns of animal development. They are important for studying homologous characteristics in the ancestor of all Metazoa prior to separation and specialization of these genes in more complex animals and for understanding the basic physiological function of cancer-related genes in simpler animals. The thesis characterizes the protein from the freshwater cave sponge Eunapius subterraneus, whose homologue in humans DRG1 (Developmentally Regulated GTP binding protein 1) is associated with cancer. The goal is to gain a better insight into the evolution of this metastatic suppressor. The cDNA encoding the DRG1 protein was successfully cloned into the pET28b expression vector and overexpression was performed. The DRG1 protein has GTPase activity that was tested by measuring the amount of unused GTP after the hydrolysis reaction. In addition, the protein was subjected to a gel filtration method to estimate its molecular weight.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:552357
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-10-28
Terms of useLicence In copyright icon
Created on2020-10-30 11:07:19