prikaz prve stranice dokumenta Diferencijalna i citokemijska analiza krvnih stanica zelene žabe (Peleophylax ridibundus, Pallas, 1771)
Access restricted to students and staff of home institution
master's thesis
Diferencijalna i citokemijska analiza krvnih stanica zelene žabe (Peleophylax ridibundus, Pallas, 1771)
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:391726
Kleiner, Iva
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Kleiner, I. (2013). Diferencijalna i citokemijska analiza krvnih stanica zelene žabe (Peleophylax ridibundus, Pallas, 1771) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:391726

Kleiner, Iva. "Diferencijalna i citokemijska analiza krvnih stanica zelene žabe (Peleophylax ridibundus, Pallas, 1771)." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:391726

Kleiner, Iva. "Diferencijalna i citokemijska analiza krvnih stanica zelene žabe (Peleophylax ridibundus, Pallas, 1771)." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:391726

Kleiner, I. (2013). 'Diferencijalna i citokemijska analiza krvnih stanica zelene žabe (Peleophylax ridibundus, Pallas, 1771)', Master's thesis, University of Zagreb, Faculty of Science, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:391726

Kleiner I. Diferencijalna i citokemijska analiza krvnih stanica zelene žabe (Peleophylax ridibundus, Pallas, 1771) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:391726

I. Kleiner, "Diferencijalna i citokemijska analiza krvnih stanica zelene žabe (Peleophylax ridibundus, Pallas, 1771)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:391726

Metadata
TitleDiferencijalna i citokemijska analiza krvnih stanica zelene žabe (Peleophylax ridibundus, Pallas, 1771)
Title (english)Differential and cytochemical analysis of blood cells in green frogs (Pelophylax ridibundus, Pallas, 1771)
AuthorIva Kleiner
MentorDomagoj Đikić (mentor)
Committee memberDomagoj Đikić (predsjednik povjerenstva)
Committee memberGordana Lacković-Venturin (član povjerenstva)
Committee memberRenata Šoštarić (član povjerenstva)
Committee memberDavor Zanella (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-02-07, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
U ovom radu koristila se puna krv zelene žabe Pelophylax ridibundus. Izračunala se brojnost eritrocita, leukocita te vrijednost hematokrita. Morfološka i kvantitativna analiza krvnih stanica provedena je bojanjem May-Grünwald Giemsom. Diferencijalnim citokemijskim tehnikama napravljena je citološka karakterizacija različitih tipova leukocita. Glikokonjugati i polisaharidi dokazani su tehnikama alcian blue (AB) i periodic acid Schiff (PAS). Stanični lipidi dokazani su tehnikom sudan black B (SBB). Enzimatsko-citokemijskim tehnikama dokazala se aktivnost alfa-naftil acetat esteraza (ANAE), alfa-naftil butirat esteraza (ANBE) te alkalne fosfataze (ALP). Brojnost i udio leukocita, hematokrit i morfologija stanica slični su ostalim žabama, dok je brojnost eritrocita veća. Bojanje ABom imaju jezgre svih stanica, citoplazma limfocita, monocita, dio neutrofila, trombocita i neke granule eozinofila. PASom se boje limfociti, granule eozinofila, neki monociti, neutrofili i trombociti. SBBom se boje leukociti, trombociti, slabije nezreli i zreli eritrociti. Aktivnost ANAE pokazuju monociti i neki limfociti. Aktivnost ANBE imaju monociti, neki trombociti i limfociti. ALP nije dala pozitivne rezultate.
Abstract (english)
This thesis presents results attained from peripheral blood of the frog Pelophylax ridibundus. It contains hematocrit values, erythrocytes and leukocytes counts. The May-Grünwald Giemsom stain was used in morphological and quantitative blood cell analysis. Cytological characterization of leukocytes types used differential cytochemical stains. The alcian blue (AB) dye and the periodic acid Schiff method (PAS) stained glycoconjugates and polysaccharides. The sudan black B (SBB) dye stained cell lipids. Activities of alpha-naphthyl acetate esterase (ANAE), alpha-naphthyl butyrate esterase (ANBE) and alkaline phosphatase (ALP) were proved using enzyme-cytochemical reactions. Leukocyte count and percentage, hematocrit and cell morphology are similar to other frogs, unlike erythrocyte counts which are greater. AB stained nuclei of all cells, cytoplasm of lymphocytes, monocytes, some neutrophils, thrombocytes and some eosinophil granules. PAS stained lymphocytes, eosinophil granules, some monocytes, neutrophils and thrombocytes. All leukocytes, thrombocytes, young and mature erythrocytes stained with SBB. Monocytes and lymphocytes have active ANAE. Monocytes, some lymphocytes and thrombocytes have active ANBE. ALP activity was not recorded.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:391726
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 09:10:25