prikaz prve stranice dokumenta Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG
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master's thesis
Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:496612
Kovačević, Jelena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Kovačević, J. (2013). Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:496612

Kovačević, Jelena. "Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:496612

Kovačević, Jelena. "Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:496612

Kovačević, J. (2013). 'Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG', Master's thesis, University of Zagreb, Faculty of Science, accessed 16 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:496612

Kovačević J. Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 May 16] Available at: https://urn.nsk.hr/urn:nbn:hr:217:496612

J. Kovačević, "Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:496612

Metadata
TitleTransgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG
Title (english)Generation of Arabidopsis thaliana (Heynh.) plants containing the TROL protein labeled with HA and FLAG protein markers
AuthorJelena Kovačević
MentorHrvoje Fulgosi (mentor)
Committee memberNataša Bauer (predsjednik povjerenstva)
Committee memberHrvoje Fulgosi (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberDijana Škorić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-02-05, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Transformacija biljaka metodom “floral dip” postupak je unosa T-DNA sekvence u genom stanica ovarija prije oplodnje. U ovom radu opisala sam transformaciju biljaka Arabidopsis thaliana molekulom T-DNA iz plazmidnog vektora pH7WG2,0 TROL-HA-FLAG. TROL je transmembranski protein koji sudjeluje u regulaciji puta prijenosa elektrona na tilakoidnim membranama kloroplasta. Dodatak proteinskih biljega HA i FLAG na protein TROL olakšava njegovu imunodetekcijsku provjeru i omogućuje daljnja istraživanja funkcije proteina TROL. Kako bih dokazala uspješnost transformacije biljaka genom za protein TROL-HA-FLAG, biljke sam analizirala metodama selekcije sjemenki na hranjivoj podlozi s antibiotikom, PCR provjere genoma i imunodetekcije proteina u listovima odraslih biljaka. U transformiranim biljkama sam imunodetekcijom potvrdila lokalizaciju proteina TROL-HA-FLAG u tilakoidnim membranama kloroplasta. Daljnja istraživanja omogućit će detaljniju potvrdu funkcionalnosti proteina TROL-HA-FLAG.
Abstract (english)
Floral dip is a transformation method that results in the insertion of T-DNA into the genome of unfertilized ovary cells. This thesis describes the transformation of Arabidopsis thaliana plants with T-DNA from pH7WG2,0 TROL-HA-FLAG plasmid vector. TROL is a transmembrane protein that regulates electron transport flow on the thylakoid membranes of chloroplasts. Addition of protein tags HA and FLAG to the TROL protein allows easier immunodetection analysis and enables further studies of the plant TROL protein function. In order to show the success of plant transformation with the TROL-HA-FLAG protein gene, the plants were subjected to multiple analyses such as seed selection method on growth plates with an added antibiotic, plant DNA PCR analysis and immunodetection analysis of plant leaf proteins. Due to the immunodetection analysis, I managed to confirm the localization of TROL-HA-FLAG protein in chloroplast thylakoid membranes of transformed plants. Further research could better show if TROL-HA-FLAG protein function is diminished.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:496612
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 09:26:49