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master's thesis
Utjecaj šaperona HtpG na količine proteina Cas3 i zaštitu od infekcije fagom lambda u ovisnosti o temperaturi u bakteriji Escherichia coli
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:474070
Pravica, Mihaela
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Pravica, M. (2020). Utjecaj šaperona HtpG na količine proteina Cas3 i zaštitu od infekcije fagom lambda u ovisnosti o temperaturi u bakteriji Escherichia coli (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:474070

Pravica, Mihaela. "Utjecaj šaperona HtpG na količine proteina Cas3 i zaštitu od infekcije fagom lambda u ovisnosti o temperaturi u bakteriji Escherichia coli." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:474070

Pravica, Mihaela. "Utjecaj šaperona HtpG na količine proteina Cas3 i zaštitu od infekcije fagom lambda u ovisnosti o temperaturi u bakteriji Escherichia coli." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:474070

Pravica, M. (2020). 'Utjecaj šaperona HtpG na količine proteina Cas3 i zaštitu od infekcije fagom lambda u ovisnosti o temperaturi u bakteriji Escherichia coli', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:474070

Pravica M. Utjecaj šaperona HtpG na količine proteina Cas3 i zaštitu od infekcije fagom lambda u ovisnosti o temperaturi u bakteriji Escherichia coli [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:474070

M. Pravica, "Utjecaj šaperona HtpG na količine proteina Cas3 i zaštitu od infekcije fagom lambda u ovisnosti o temperaturi u bakteriji Escherichia coli", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:474070

Metadata
TitleUtjecaj šaperona HtpG na količine proteina Cas3 i zaštitu od infekcije fagom lambda u ovisnosti o temperaturi u bakteriji Escherichia coli
Title (english)The effect of HtpG chaperone on Cas3 protein levels and protection against phage lambda infection depending on the temperature in Escherichia coli
AuthorMihaela Pravica
MentorIvana Ivančić Baće (mentor)
Committee memberIvana Ivančić Baće (predsjednik povjerenstva)
Committee memberMirela Sertić Perić (član povjerenstva)
Committee memberDraginja Mrvoš-Sermek (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2020-11-13, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Sustav CRISPR-Cas je važan mehanizam obrane mnogih prokariota od infekcije stranom DNA poput faga i plazmida. Sastoji se od lokusa CRISPR koji sadrži ponavljajuće palindromske sekvence DNA odvojene razmaknicama koje potječu od strane DNA faga ili plazmida te gena cas čijom ekspresijom nastaju proteini koji sudjeluju u obrani od infekcije. U bakteriji Escherichia coli efikasnost zaštite bakterija od faga sustavom CRISPR-Cas ovisi o aktivnosti proteina Cas3 koja pak ovisi o temperaturi inkubiranja i šaperonu HtpG. U ovom radu je istraživano utječe li šaperon HtpG na količinu ili konformaciju proteina Cas3 u ovisnosti o temperaturi. Utjecaj suviška proteina HtpG na količinu proteina Cas3 određen je metodom Western-blot, a utjecaj na konformaciju proteina Cas3 određen je mjerenjem efikasnosti zaštite određenih točkastih mutanata cas3 od infekcije fagom λ. Dobiveni rezultati ukazuju da šaperon HtpG povećava količinu proteina Cas3 te da potiče smatanje točkastih mutanata Cas3, ali samo na 30 °C.
Abstract (english)
For many prokaryotes, the CRISPR-Cas system is an important defense mechanism against foreign DNA infections, such as phages or plasmids. It is composed of the locus CRISPR, which consists of short palindromic DNA sequences separated by spacers derived from foreign DNA phages or plasmids, and cas genes whose expression produces Cas proteins that participate in the defense against infection. In bacteria Escherichia coli the efficiency of protection of the bacteria against phages by the CRISPR-Cas system depends on the activity of the Cas3 protein, which depends on the incubation temperature and HtpG chaperone. In this work, we investigated whether the HtpG chaperone influences the amount or conformation of the Cas3 protein as a function of temperature. The influence of elevated HtpG levels on the amounts of the Cas3 protein is determined using the Western blot method, and the influence on the conformation of the Cas3 protein is determined by measuring the efficiency of protection of specific cas3 point mutants against infection with the λ phage. The results suggest that the HtpG chaperone increases the amounts of Cas3 protein and induces the folding of Cas3 point mutants, but only at 30 °C.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:217:474070
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-11-13
Terms of useLicence In copyright icon
Created on2020-11-26 07:51:43