prikaz prve stranice dokumenta Genska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822)
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master's thesis
Genska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822)
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:746474
Krznar, Petra
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Krznar, P. (2013). Genska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:746474

Krznar, Petra. "Genska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822)." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:746474

Krznar, Petra. "Genska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822)." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:746474

Krznar, P. (2013). 'Genska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822)', Master's thesis, University of Zagreb, Faculty of Science, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:746474

Krznar P. Genska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:217:746474

P. Krznar, "Genska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:746474

Metadata
TitleGenska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822)
Title (english)Expression profiling and phylogeny of MATE (Multidrug and toxin extrusion) proteins in zebrafish, Danio rerio (Hamilton, 1822.)
AuthorPetra Krznar
MentorTvrtko Smital (mentor)
MentorGoran Klobučar (mentor)
Committee memberGoran Klobučar (predsjednik povjerenstva)
Committee memberTvrtko Smital (član povjerenstva)
Committee memberKristian Vlahoviček (član povjerenstva)
Committee memberMirjana Kalafatić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-02-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Apsorpcija, distribucija, transformacija i eliminacija predstavljaju ključne dijelove metabolizma endo- i ksenobiotika. U posljednjem koraku eliminacije sudjeluju i tzv. MATE (Multidrug and toxin extrusion) proteini koji izbacuju kemikalije iz stanice. MATE proteini su pronađeni u gotovo svim organizmima, od bakterija do ljudi. Kod ljudi su pronađena tri MATE proteina (MATE1, MATE2, MATE2-K), dok je kod većine kralježnjaka pronađeno dva MATE proteina. MATE geni su prisutni u svim tkivima, pri čemu su najviše eksprimirani u bubrezima i jetri, najvažnijim detoksikacijskim organima. Trenutno znanje o MATE proteinima je bazirano isključivo na MATE proteinima sisavaca. Stoga smo željeli istražiti MATE proteine riba, koristeći zebricu (Danio rerio, Hamilton, 1822.) kao modelni organizam. Prikazali smo prvu komparativnu filogeniju MATE proteina od bakterija do ljudi i identificirali pet MATE gena u zebrici. MATE geni u zebrici su dodatno karakterizirani određivanjem ekspresije mRNA u tkivima muških i ženskih riba.
Abstract (english)
Absorption, distribution, transformation and elimination present key processes of metabolism of endo- and xenobiotics. The last step of their elimination includes so-called MATE (Multidrug and toxin extrusion) proteins which efflux chemicals from the cell. MATE proteins are found in almost all organisms, ranging from bacteria to humans. Three MATE proteins are found in humans (MATE1, MATE2, MATE2-K), while in most vertebrates two MATEs are found. MATE genes are present ubiquitously throughout body and are mostly expressed in kidneys and liver, the most important detoxification organs. Since current knowledge about MATEs is limited to mammals, the idea behind this study was to explore MATEs in fish using zebrafish (Danio rerio, Hamilton, 1822) as a model species. First comprehensive phylogenetic analysis of MATEs, from bacteria to humans have been conducted and five MATE genes have been identified in zebrafish. Five zebrafish MATEs where further characterized in terms of their mRNA expression across tissues of male and female fish.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:746474
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 09:53:51