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doctoral thesis
Uloga mitohondrijskih proteina rasprezanja u mehanizmu prijenosa protona kroz unutrašnju mitohondrijsku membranu
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:020937
Škulj, Sanja
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Škulj, S. (2020). Uloga mitohondrijskih proteina rasprezanja u mehanizmu prijenosa protona kroz unutrašnju mitohondrijsku membranu (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:020937

Škulj, Sanja. "Uloga mitohondrijskih proteina rasprezanja u mehanizmu prijenosa protona kroz unutrašnju mitohondrijsku membranu." Doctoral thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:020937

Škulj, Sanja. "Uloga mitohondrijskih proteina rasprezanja u mehanizmu prijenosa protona kroz unutrašnju mitohondrijsku membranu." Doctoral thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:020937

Škulj, S. (2020). 'Uloga mitohondrijskih proteina rasprezanja u mehanizmu prijenosa protona kroz unutrašnju mitohondrijsku membranu', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:020937

Škulj S. Uloga mitohondrijskih proteina rasprezanja u mehanizmu prijenosa protona kroz unutrašnju mitohondrijsku membranu [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:020937

S. Škulj, "Uloga mitohondrijskih proteina rasprezanja u mehanizmu prijenosa protona kroz unutrašnju mitohondrijsku membranu", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:020937

Metadata
TitleUloga mitohondrijskih proteina rasprezanja u mehanizmu prijenosa protona kroz unutrašnju mitohondrijsku membranu
Title (english)The role of uncoupling proteins in the proton transfer mechanism across the inner mitochondrial membrane
AuthorSanja Škulj
MentorMario Vazdar (mentor)
Committee memberBranimir Bertoša (predsjednik povjerenstva)
Committee memberDavor Margetić (član povjerenstva)
Committee memberBorislav Kovačević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2020-11-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
Prijenos protona kroz unutrašnju mitohondrijsku membranu dio je povezanih lančanih mehanizama koji su važni za normalno usklađeno funkcioniranje organizma. Većinski dio prijenosa protona odvija se uz pomoć transmembranskih proteina ADP/ATP translokatora (AAC) i mitohondrijskih proteina rasprezanja (UCP1-5). U radu je naglasak stavljen na mitohondrijski protein rasprezanja 2 (UCP2) za koji je predložena tercijarna struktura, a čija glavna uloga je kontrola oksidativnog stresa u organizmu. Uz protein za prijenos protona potrebna je i dugolančana masna kiselina koja se uz malu energetsku barijeru translocira kroz unutrašnju mitohondrijsku membranu u neutralnoj formi, dok je povratak anionske forme potpomognut proteinom. Drugi dio istraživanja vezan je uz utjecaj oksidativnog stresa gdje je detaljno opisana modelna reakcija produkata lipidne peroksidacije reaktivnih aldehida 4- hidroksi-2-nonenala (HNE-a) i 4-okso-2-nonenala (ONE-a) s reaktivnim aminokiselinama iz proteina – lizinom, argininom, histidinom i cisteinom te fosfatidiletanolaminskim (PE) lipidom koji je sastavni dio unutarnje mitohondrijske membrane.
Abstract (english)
Proton transfer across the inner mitochondrial membrane is part of the related chain mechanisms that are important for normal functioning of the organism. Majority of proton transfers are mediated by transmembrane protein ADP/ATP translocator (AAC) and via mitochondrial uncoupling proteins 1-5 (UCP1-5). This thesis focuses primarily on mitochondrial uncoupling protein 2 (UCP2) for which we proposed a tertiary structure, and whose main role is control of the oxidative stress in the body. In addition to the protein accommodating proton transfer, this process requires a presence of a long chain fatty acid, which is translocated through the inner mitochondrial membrane in its neutral form with a small energy barrier. Fatty acid returns to the cytosolic side of the membrane in its anionic form and this passage is supported by the protein. The second part of the research is related to the influence of oxidative stress, where we describe in detail the model reactions of two lipid peroxidation products, i.e. reactive aldehydes 4-hydroxy-2-nonenal (HNE) and 4-oxo-2- nonenal (ONE), with reactive amino acids from proteins, namely lysine, arginine, histidine and cysteine as well as phosphatidilethanolamine (PE) lipid which is a constitutive part of inner mitochonodrial membrane.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:020937
Promotion2020
Study programmeTitle: Doctoral study in chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
Type of resourceText
Extent140 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-12-08 13:53:31