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master's thesis
De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:429037
Boštjančić, Ljudevit Luka
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Boštjančić, Lj. L. (2021). De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:429037

Boštjančić, Ljudevit Luka. "De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:429037

Boštjančić, Ljudevit Luka. "De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:429037

Boštjančić, Lj. L. (2021). 'De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:429037

Boštjančić LjL. De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:429037

Lj. L. Boštjančić, "De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:429037

Metadata
TitleDe novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906
Title (english)De novo assembly of Procambarus virginalis Lyko, 2017 transcriptome and gene expression in response to the pathogen Aphanomyces astaci Shikora, 1906
AuthorLjudevit Luka Boštjančić
MentorKathrin Theissinger (mentor)
MentorIvana Maguire (sumentor)
Committee memberIvana Maguire (predsjednik povjerenstva)
Committee memberMarin Ježić (član povjerenstva)
Committee memberRosa Karlić (član povjerenstva)
Committee memberSandra Hudina (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-01-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Jedna on najveći prijetnji za populacije nativnih europskih slatkovodnih rakova je patogen Aphanomyces astaci Shikora, 1906 iz razreda Oomycetes, koji uzrokuje bolest račju kugu letalnu za nativne vrste slatkovodnih rakova. Mramorni rak, Procambarus virginalis Lyko, 2017 invazivna je vrsta i poznati prenositelj račje kuge. U ovom istraživanju proučen je imunosni odgovor 30 jedinki mramornog raka na infekciju s A. astaci. Korištena su dva soja patogena; PsI- visoko virulentan i As- nisko virulentan. Uzorkovanje hepatopankreasa rakova iz pokusa provedeno je tri i 21 dan nakon izlaganja patogenu te je provedena izolacija RNA. Iz fragmenata RNA, dobivenih sekvenciranjem, sastavljan je de novo transkriptom visoke kvalitete kojeg čini 60,004 gena. Za 41.9% transkripata je pronađena anotacija. Analiza diferencijalne ekspresije otkrila je 102 diferencijalno eksprimirana gena. Većina diferencijalno eksprimiranih gena bila je među uzorcima jedinki uzorkovanih 21 dana nakon infekcije. Među diferencijalno eksprimiranim genima bili su prisutni poznati efektori urođene imunosti rakova: serpin, lektini tipa-C i crustini. Novootkriveni su i geni s potencijalnom ulogom u urođenoj imunosti rakova. Oni predstavljaju predmet za buduća istraživanja urođene imunosti rakova
Abstract (english)
One of the biggest threats for freshwater crayfish populations is the pathogen Aphanomyces astaci Shikora, 1906 from the class Oomycetes that causes a disease lethal for native crayfish species, crayfish plague. The marbled crayfish, Procambarus virginalis Lyko, 2017 is an invasive crayfish species, known to be resistant to the crayfish plague disease. In this study the immune response to an A. astaci infection of 30 marbled crayfish exposed to two strains of A. astaci (PsI- high virulence and As- low virulence) was investigated. Hepatopancreas samples were taken at two time points, three days and 21 days post challenge. From the RNA sequencing reads, high-quality de novo transcriptomes of marbled crayfish were assembled containing 60,004 genes. Annotation was available for 41.9% of the transcripts. Differential gene expression analysis revealed in total 102 differentially expressed genes. More differentially expressed genes were observed among the samples from the second time point. Among the differentially expressed genes several known effectors of the crayfish innate immunity were detected. Previously unreported genes with potential role in crayfish innate immunity were also identified. They represent targets for future studies on crayfish innate immunity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:429037
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-01-25
Terms of useLicence In copyright icon
Created on2021-01-28 11:20:00