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master's thesis
Priprava, karakterizacija i interakcije 3-aminopropil dekladinozil derivata azitromicina s ribosomom iz bakterije Escherichia coli
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:785839
Gašperov, Jana
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Gašperov, J. (2020). Priprava, karakterizacija i interakcije 3-aminopropil dekladinozil derivata azitromicina s ribosomom iz bakterije Escherichia coli (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:785839

Gašperov, Jana. "Priprava, karakterizacija i interakcije 3-aminopropil dekladinozil derivata azitromicina s ribosomom iz bakterije Escherichia coli." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:785839

Gašperov, Jana. "Priprava, karakterizacija i interakcije 3-aminopropil dekladinozil derivata azitromicina s ribosomom iz bakterije Escherichia coli." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:785839

Gašperov, J. (2020). 'Priprava, karakterizacija i interakcije 3-aminopropil dekladinozil derivata azitromicina s ribosomom iz bakterije Escherichia coli', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:785839

Gašperov J. Priprava, karakterizacija i interakcije 3-aminopropil dekladinozil derivata azitromicina s ribosomom iz bakterije Escherichia coli [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:785839

J. Gašperov, "Priprava, karakterizacija i interakcije 3-aminopropil dekladinozil derivata azitromicina s ribosomom iz bakterije Escherichia coli", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:785839

Metadata
TitlePriprava, karakterizacija i interakcije 3-aminopropil dekladinozil derivata azitromicina s ribosomom iz bakterije Escherichia coli
Title (english)Synthesis, characterization and interactions of 3-aminopropyl descladinosyl derivate of azithromycin with Escherichia coli ribosome
AuthorJana Gašperov
MentorPredrag Novak (mentor)
Committee memberPredrag Novak (predsjednik povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberIvica Đilović (član povjerenstva)
Committee memberTomislav Jednačak (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2020-12-08, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Makrolidni antibiotici, poput azitromicina, koriste se u liječenju infekcija uzrokovanih Gram-pozitivnim i Gram-negativnim bakterijama. Djeluju kao inhibitori sinteze bakterijskih proteina tako što se vežu na 50S podjedinicu bakterijskog ribosoma u blizini centra peptidil-transferaze. Učestalo korištenje makrolidnih antibiotika dovelo je do pojave i razvoja bakterijske rezistencije. Iz tog razloga javlja se potreba za otkrićem i razvojem novih antibiotika. Za razumijevanje cjelokupnog biološkog profila novog potencijalnog lijeka važno je odrediti konformaciju molekule u slobodnom i vezanom stanju te istražiti interakcije s biološkim metama. U ovom radu uspješno je pripravljen 3-aminopropil dekladinozil derivat azitromicina (S6), prekursor u sintezi makrozona, novih bioaktivnih konjugata azitromicina i tiosemikarbazona. Pripravljeni spoj okarakteriziran je spektroskopijom NMR i spektrometrijom masa. Upotrebom tehnike ROESY određena je konformacija prekursora u slobodnom stanju, a pomoću tehnika tr-NOESY i STD istražene su interakcije prekursora s ribosomom izoliranim iz bakterije Escherichia coli te je određena konformacija u vezanom stanju.
Abstract (english)
Macrolide antibiotics, such as azithromycin, are used to treat infections caused by Gram-positive and Gram-negative bacteria. They act as inhibitors of bacterial protein synthesis by binding to the 50S subunit of the bacterial ribosome at or near the peptidyl transferase center. Frequent use of macrolide antibiotics has led to the emergence of bacterial resistance. For this reason, there is a need to discover and develop new antibiotics. To understand the overall biological profile of a new potential drug, it is important to determine the conformation of molecules in the free and bound state and to investigate interactions with biological targets. In this work, 3-aminopropyl decladinosyl derivative of azithromycin (S6), a precursor in the synthesis of macrozones, a new bioactive conjugate of azithromycin and thiosemicarbazone, was successfully prepared. The compound S6 was characterized by NMR spectroscopy and mass spectrometry. ROESY technique was applied to estimate free conformation in solution. The interactions of S6 with the ribosome isolated from Escherichia coli were investigated by using tr-NOESY and STD techniques. The analysis of the recorded spectra enabled determination of conformation in bound state.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:785839
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-02-15 11:55:05