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master's thesis
Uloga p97/VCP AAA ATPaze u popravku unakrsnog vezanja DNA i proteina
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:726279
Dakić, Valentin
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Dakić, V. (2020). Uloga p97/VCP AAA ATPaze u popravku unakrsnog vezanja DNA i proteina (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:726279

Dakić, Valentin. "Uloga p97/VCP AAA ATPaze u popravku unakrsnog vezanja DNA i proteina." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:726279

Dakić, Valentin. "Uloga p97/VCP AAA ATPaze u popravku unakrsnog vezanja DNA i proteina." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:726279

Dakić, V. (2020). 'Uloga p97/VCP AAA ATPaze u popravku unakrsnog vezanja DNA i proteina', Master's thesis, University of Zagreb, Faculty of Science, accessed 27 July 2024, https://urn.nsk.hr/urn:nbn:hr:217:726279

Dakić V. Uloga p97/VCP AAA ATPaze u popravku unakrsnog vezanja DNA i proteina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 July 27] Available at: https://urn.nsk.hr/urn:nbn:hr:217:726279

V. Dakić, "Uloga p97/VCP AAA ATPaze u popravku unakrsnog vezanja DNA i proteina", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:726279

Metadata
TitleUloga p97/VCP AAA ATPaze u popravku unakrsnog vezanja DNA i proteina
Title (english)The role of p97/VCP AAA ATPase in the repair of DNA-protein crosslinks
AuthorValentin Dakić
MentorMarta Popović (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2020-12-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Unakrsna veza između DNA i proteina (engl. DNA-protein crosslink, DPC) je tip oštećenja DNA koje nastaje kao posljedica stvaranja ireverzibilne kovalentne veze između DNA i proteina u prisutnosti endogenog ili egzogenog DPC-inducirajućeg agensa. Nemogućnost popravka DPC-a uzrokuje genomsku nestabilnost i teške posljedice za organizam, uključujući preuranjeno starenje, neurodegeneraciju i pojavu tumora. ATPaza p97 tvori kompleks s glavnim čimbenikom popravka DPC-a, SPRTN metaloproteazom. Međutim, uloga p97 u ovom putu DNA popravka nije poznata, te je stoga cilj ovog rada istražiti sudjeluje li i na koji način p97 u popravku DPC-a i u kakvom je odnosu sa SPRTN proteazom. U tu su svrhu prekomjerno eksprimirani i pročišćeni humani proteini p97 i njegov interaktor SPRTN radi kasnije strukturne i biokemijske analize njihovog kompleksa krio-elektronskom mikroskopijom. Provedena je i analiza ekspresije p97 i SPRTN gena (na razini mRNA) u tkivima dvaju modelnih organizama, ribe zebrice (Danio rerio) i domaćeg miša (Mus musculus), kako bi se usporedio ekspresijski profil p97 i SPRTN gena kroz tkiva te među organizmima.
Abstract (english)
DNA-protein crosslink (DPC) is a type of DNA damage that occurs as a result of the formation of an irreversible covalent bond between DNA and protein in the presence of an endogenous or exogenous DPC-inducing agent. The inability to repair DPCs causes genomic instability and severe consequences for the organism, including premature aging, neurodegeneration, and tumor appearance. ATPase p97 forms a complex with the major DPC repair factor, SPRTN metalloprotease. However, the role of p97 in this pathway of DNA repair is unknown, and therefore the aim of this thesis is to get closer to answering whether and in what way p97 participates in DPC repair and what is its relation to SPRTN protease. For this purpose, human proteins p97 and its interactor SPRTN were overexpressed and purified for subsequent structural and biochemical analysis of their complex by cryo-electron microscopy. Expression analysis of p97 and SPRTN genes (at the mRNA level) in the tissues of two model organisms, zebrafish (Danio rerio) and mice (Mus musculus) was performed to compare the expression profile of p97 and SPRTN genes across tissues and organisms.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:726279
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-12-22
Terms of useLicence In copyright icon
Created on2021-02-16 12:23:14