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master's thesis
Karakterizacija tirozil-DNA-fosfodiesteraza (Tdp1 i Tdp2) u popravku unakrsne veze DNA i proteina u modelnom organizmu ribe zebrice
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:415343
Jukić, Luka
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Jukić, L. (2021). Karakterizacija tirozil-DNA-fosfodiesteraza (Tdp1 i Tdp2) u popravku unakrsne veze DNA i proteina u modelnom organizmu ribe zebrice (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:415343

Jukić, Luka. "Karakterizacija tirozil-DNA-fosfodiesteraza (Tdp1 i Tdp2) u popravku unakrsne veze DNA i proteina u modelnom organizmu ribe zebrice." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:415343

Jukić, Luka. "Karakterizacija tirozil-DNA-fosfodiesteraza (Tdp1 i Tdp2) u popravku unakrsne veze DNA i proteina u modelnom organizmu ribe zebrice." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:415343

Jukić, L. (2021). 'Karakterizacija tirozil-DNA-fosfodiesteraza (Tdp1 i Tdp2) u popravku unakrsne veze DNA i proteina u modelnom organizmu ribe zebrice', Master's thesis, University of Zagreb, Faculty of Science, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:415343

Jukić L. Karakterizacija tirozil-DNA-fosfodiesteraza (Tdp1 i Tdp2) u popravku unakrsne veze DNA i proteina u modelnom organizmu ribe zebrice [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:217:415343

L. Jukić, "Karakterizacija tirozil-DNA-fosfodiesteraza (Tdp1 i Tdp2) u popravku unakrsne veze DNA i proteina u modelnom organizmu ribe zebrice", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:415343

Metadata
TitleKarakterizacija tirozil-DNA-fosfodiesteraza (Tdp1 i Tdp2) u popravku unakrsne veze DNA i proteina u modelnom organizmu ribe zebrice
Title (english)Characterisation of tyrosyl-DNA phosphodiesterases (Tdp1 and Tdp2) in DNA-protein crosslink repair in zebrafish model organism
AuthorLuka Jukić
MentorMarta Popović (mentor)
Committee memberMarko Močibob (predsjednik povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberMorana Dulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-02-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Postoje brojna oštećenja DNA koja mogu nastajati na različite načine, bilo endogeno ili egzogeno. Jedan od načina oštećenja DNA je nastajanje DNA-protein unakrsnih vezanja, oštećenja karakterističnih po postojanju kovalentne veze između proteina i DNA. Tirozil-DNA-fosfodiesteraze (Tdp) su proteini koji kataliziraju hidrolizu fosfotirozilne veze popravljajući DNA-protein unakrsnih vezanja nastala ireverzibilnim povezivanjem enzima topoizomeraza i DNA. U svrhu ovog rada određene su razine genske ekspresije tirozil-DNA-fosfodiesteraze 1 i tirozil-DNA-fosfodiesteraze 2 u tkivima mužjaka i ženki miševa kvantitativnom lančanom reakcijom polimerazom te su iste međusobno uspoređene. Razina ekspresije također je uspoređena s ekspresijom tirozil-DNA-fosfodiesteraza određenoj u ribi zebrici i čovjeku. Uz određivanje ekspresije provedena je identifikacija utemeljitelja Tdp1-/- linije riba zebrica koristeći metodu analize mekšanja visoke rezolucije.
Abstract (english)
There are numerous types of DNA damage that can occur in a variety of ways, either endogenously or exogenously. One of the types of DNA damage is the formation of DNA-protein cross-links, damage characterized by the existence of a covalent bond between protein and DNA. Tyrosyl-DNA phosphodiesterases (Tdps) are proteins that catalyze the hydrolysis of the phosphotyrosyl bond by repairing the DNA-crosslinked protein formed by the irreversible binding of the enzyme topoisomerases and DNA. For the purpose of this work, the levels of gene expression of tyrosyl-DNA phosphodiesterase 1 and tyrosyl-DNA phosphodiesterase 2 in the tissues of male and female mice were determined by quantitative polymerase chain reaction and they were compared with each other. The expression levels in mice were also compared to the expression levels of tyrosyl-DNA phosphodiesterases in zebrafish and human. In addition to determining expression founder identification of zebrafish Tdp1-/- line was performed using the high resolution melting analysis method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:415343
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-02-16
Terms of useLicence In copyright icon
Created on2021-03-01 14:28:46