prikaz prve stranice dokumenta Učinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša
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master's thesis
Učinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:171428
Marinčić, Dragica
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Marinčić, D. (2021). Učinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:171428

Marinčić, Dragica. "Učinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:171428

Marinčić, Dragica. "Učinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:171428

Marinčić, D. (2021). 'Učinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:171428

Marinčić D. Učinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:171428

D. Marinčić, "Učinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:171428

Metadata
TitleUčinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša
Title (english)Effect of systemic hyperthermia on the antitumor potential of cisplatin and resveratrol nanocrystals on Ehrlich ascites solid tumor in mouse
AuthorDragica Marinčić
MentorNada Oršolić (mentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-02-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cilj ovog istraživanja bio je istražiti sinergistički učinak sustavne hipertermije uz nanokristale resveratrola i cisplatinu na inhibiciju brzorastućeg EAT, preživljavanje miševa, polarizaciju makrofaga te sposobnost nanokristala resveratrola u smanjenju toksičnih učinaka cisplatine i hipertermije u jetri i bubregu miševa. Miševima soja Swiss albino izazvan je tumor injiciranjem 1x106 stanica EAT subkutano u bedro, a zatim su obrađeni injiciranjem nanokristala resveratrola u dozi 50 mg/kg per os tijekom 5 dana u nizu te cisplatine u dozi 2,5 mg/kg 10. i 12. dan te u dozi 5 mg/kg 14. dan uz izlaganje sustavnoj hipertermiji pri 41°C. Dobiveni rezultati pokazuju da samostalna primjena nanokristala resveratrola i izlaganje hipertermiji vodi rastu tumora dok primjena nanokristala resveratrola i cisplatine uz izlaganje sustavnoj hipertermiji ostvaruje izraziti antitumorski učinak inhibicijom rasta EAT u solidnom obliku, ali ne pridonosi znatnijem povećanju preživljavanja životinja. Injiciranje cisplatine pri hipertermalnim uvjetima povećava životni vijek miševa za 47% u odnosu na kontrolu. Temeljem dobivenih rezultata možemo zaključiti da nanokristali resveratrola mogu polučiti antioksidacije i/ili prooksidacijske učinke u različitim mikrookolišnim uvjetima, ali i sudjelovati u povećanju makrofagne plastičnosti i polarizacije te zaštititi bubreg i jetru od toksičnosti izazvane cisplatinom.
Abstract (english)
The aim of this study was to investigate the synergistic effect of systemic hyperthermia with resveratrol nanocrystals and cisplatin on the inhibition of fast-growing EAT, mouse survival, macrophage polarization, and the ability of resveratrol nanocrystals to reduce the toxic effects of cisplatin and hyperthermia. In Swiss albino mice tumor were induced by injecting 1x106 EAT cells subcutaneously into the thigh and then treated by injecting resveratrol nanocrystals at a dose of 50 mg/kg per os for 5 consecutive days and cisplatin at a dose of 2,5 mg/kg day 10 and 12 and at a dose of 5 mg/kg on day 14 with exposure to systemic hyperthermia at 41°C. The results show that the use of resveratrol nanocrystals alone and exposure to hyperthermia leads to tumor growth, while the use of resveratrol and cisplatin nanocrystals with exposure to systemic hyperthermia has a pronounced antitumor effect by inhibiting EAT growth in solid form, but does not significantly increase animal survival. Injection of cisplatin under hyperthermal conditions increased the lifespan of mice by 47% compared to control. Based on the obtained results, we can conclude that resveratrol nanocrystals have antioxidant and/or prooxidative effects in various microenvironmental conditions, but also participate in increasing macrophage plasticity and polarization and protect the kidney and liver from cisplatin-induced toxicity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:171428
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-02-26
Terms of useLicence In copyright icon
Created on2021-03-09 08:01:31