prikaz prve stranice dokumenta Učinak gvanilinskih peptida na signalni sustav bradikinina u kulturi HEK293 stanica
Access restricted to students and staff of home institution
master's thesis
Učinak gvanilinskih peptida na signalni sustav bradikinina u kulturi HEK293 stanica
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:058889
Rabik, Karolina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Rabik, K. (2013). Učinak gvanilinskih peptida na signalni sustav bradikinina u kulturi HEK293 stanica (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:058889

Rabik, Karolina. "Učinak gvanilinskih peptida na signalni sustav bradikinina u kulturi HEK293 stanica." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:058889

Rabik, Karolina. "Učinak gvanilinskih peptida na signalni sustav bradikinina u kulturi HEK293 stanica." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:058889

Rabik, K. (2013). 'Učinak gvanilinskih peptida na signalni sustav bradikinina u kulturi HEK293 stanica', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:058889

Rabik K. Učinak gvanilinskih peptida na signalni sustav bradikinina u kulturi HEK293 stanica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:058889

K. Rabik, "Učinak gvanilinskih peptida na signalni sustav bradikinina u kulturi HEK293 stanica", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:058889

Metadata
TitleUčinak gvanilinskih peptida na signalni sustav bradikinina u kulturi HEK293 stanica
Title (english)Effects of guanylin peptides on bradykinin signaling pathway in HEK293 cell culture
AuthorKarolina Rabik
MentorAleksandra Dugandžić (mentor)
MentorZoran Tadić (mentor)
Committee memberZoran Tadić (predsjednik povjerenstva)
Committee memberAleksandra Dugandžić (član povjerenstva)
Committee memberInga Marijanović (član povjerenstva)
Committee memberMirjana Kalafatić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-12-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Okluzija cerebralne arterije dovodi do moždanog udara te posljedično moždanog edema koji pridonosi povećanju ishemijske ozljede. U ovom radu istraživati će se djelovanje gvanilinskih peptida, gvanilina i urogvanilina, agonista gvanilat ciklaze C, na signalni sustav bradikinina kao glavnog uzročnika nastanka moždanog edema nakon moždanog udara. Cilj je razjasniti mehanizam djelovanja gvanilinskih peptida na stanicama HEK293 te njihovo djelovanje na bradikinin, kao što smo nedavno prikazali za agoniste gvanilat ciklaze A, atrijski natriuretski peptid (ANP) i urodilatin. U ovom radu sam koristila metodu priklještenih potencijala (slow whole cell patch-clamp) za mjerenje membranskih potencijala HEK293 stanica, kojom smo pokazali da gvanilin i urogvanilin kao i njihov sekundarni glasnik, ciklički GMP, depolariziraju staničnu membranu i inhibiraju djelovanje bradikininskog signalnog puta preko aktivacije RGS proteina.Rezultati ovog istraživanja pretpostavljaju moguću zaštitnu ulogu gvanilinskih peptida u smanjenju moždanog edema te posljedično smanjenja veličine ishemijske ozljede mozga.
Abstract (english)
The occlusion of cerebral arteries leads to a stroke and consequently to a cerebral edema, which contributes to an increase of ischemic injury. In this study we investigate the effect of guanylin peptides, guanylin and uroguanylin and guanylate cyclase C agonist on the signaling system of bradykinin, which is the main contributor to the occurrence of cerebral edema after the stroke. The aim of this study is to clarify the activity mechanism of the guanylin peptides on HEK293 cells and their effect on bradykinin, as we have recently shown on the example of guanylate cyclase agonist A, atrial natriuretic peptide (ANP) and urodilatin. In this experiment we used slow-whole cell patch-clamp technique to measure a membrane potential in HEK293 cells. We showed that guanylin and uroguanylin, just like their secondary messenger cyclic GMP, depolarize a plasma membrane and they inhibit the activity of bradykinin signaling pathway via an activation of RGS protein. Results of this study suggest a possible protective role of guanylin peptides in a decrease of cerebral edema and also a decrease of an ischemic brain injury.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:058889
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 12:46:35