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master's thesis
Određivanje aminokiselina metodom tekućinske kromatografije visoke učinkovitosti uz detekciju ultraljubičastim zračenjem
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:839877
Belajević, Marin
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Belajević, M. (2021). Određivanje aminokiselina metodom tekućinske kromatografije visoke učinkovitosti uz detekciju ultraljubičastim zračenjem (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:839877

Belajević, Marin. "Određivanje aminokiselina metodom tekućinske kromatografije visoke učinkovitosti uz detekciju ultraljubičastim zračenjem." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:839877

Belajević, Marin. "Određivanje aminokiselina metodom tekućinske kromatografije visoke učinkovitosti uz detekciju ultraljubičastim zračenjem." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:839877

Belajević, M. (2021). 'Određivanje aminokiselina metodom tekućinske kromatografije visoke učinkovitosti uz detekciju ultraljubičastim zračenjem', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:839877

Belajević M. Određivanje aminokiselina metodom tekućinske kromatografije visoke učinkovitosti uz detekciju ultraljubičastim zračenjem [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:839877

M. Belajević, "Određivanje aminokiselina metodom tekućinske kromatografije visoke učinkovitosti uz detekciju ultraljubičastim zračenjem", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:839877

Metadata
TitleOdređivanje aminokiselina metodom tekućinske kromatografije visoke učinkovitosti uz detekciju ultraljubičastim zračenjem
Title (english)Determination of amino acids by high-performance liquid chromatography with ultraviolet detection
AuthorMarin Belajević
MentorLidija Brkljačić (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberAdriana Kenđel (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-02-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Metoda tekućinske kromatografije uz detekciju ultraljubičastim zračenjem (HPLC-UV) korištena je za određivanje odabranih aminokiselina u vodenim uzorcima uz derivatizacijski reagens 9-fluorenilmetil-klorformijat (Fmoc-Cl). Sintetizirani su Fmoc-aminokiselinski derivati i okarakterizirani spektroskopskim metodama te korišteni kao analitički standardi. Razvojem metode optimirani su kromatografski uvjeti kojim je postignuta dobra rezolucija i razdvajanje odabranih Fmoc-aminokiselinskih derivata i nusprodukata koji nastaju u derivatizacijskoj reakciji. Istraženi su i optimirani reakcijski uvjeti za osiguravanje ekvimolarnosti derivatizacijske reakcije s obzirom na poznati dodatak pojedine aminokiseline. Kromatografskom HPLC-UV metodom određen je maseni udio glutaminske kiseline (1,2 -13,4 %) u odabranim dodatcima jelima.
Abstract (english)
Liquid chromatography with ultraviolet detection (HPLC-UV) was used for the determination of selected amino acids in aqueous samples using 9-fluorenylmethyl chloroformate (Fmoc-Cl) as a derivatization reagent. Fmoc-amino acid derivatives were synthesized and characterized by spectroscopic methods. Formed products were used as analytical standards. Through the development of the HPLC method, the chromatographic conditions were optimized, which led to a fine resolution and separation of the selected Fmoc-amino acid derivatives and by-products formed in the derivatization reaction. The reaction conditions for ensuring the equimolarity of the derivatization reaction were investigated and with respect to the known addition of each amino acid. By the obtained HPLC-UV method, the mass content of glutamic acid (1,2 -13,4%) in some food additives was determined.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:839877
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-02-23
Terms of useLicence In copyright icon
Created on2021-03-10 09:58:57