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master's thesis
Metode priprave i karakterizacija liposoma s vankomicinom
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:365876
Rubinić, Barbara
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Rubinić, B. (2021). Metode priprave i karakterizacija liposoma s vankomicinom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:365876

Rubinić, Barbara. "Metode priprave i karakterizacija liposoma s vankomicinom." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:365876

Rubinić, Barbara. "Metode priprave i karakterizacija liposoma s vankomicinom." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:365876

Rubinić, B. (2021). 'Metode priprave i karakterizacija liposoma s vankomicinom', Master's thesis, University of Zagreb, Faculty of Science, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:365876

Rubinić B. Metode priprave i karakterizacija liposoma s vankomicinom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:217:365876

B. Rubinić, "Metode priprave i karakterizacija liposoma s vankomicinom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:365876

Metadata
TitleMetode priprave i karakterizacija liposoma s vankomicinom
Title (english)Preparation and characterization of Vancomycin liposomes
AuthorBarbara Rubinić
MentorErnest Meštrović (mentor)
Committee memberNikola Cindro (predsjednik povjerenstva)
Committee memberErnest Meštrović (član povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
Committee memberIvana Biljan (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-03-05, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Vankomicin je glikopeptidni antibiotik koji se koristi kod infekcija meticilin-rezistentnim Staphilococcom aureusom (MRSA). Vankomicin može uzrokovati nefrotokičnost u ovisnosti o primjenjenoj dozi, do 40% slučajeva. Primjena vankomicina u formulaciji s liposomom, sfernom lipidnom vezikulom, pokazuje smanjeno zadržavanje vankomicina u bubregu i stoga smanjenu nefrotoksičnost. Liposomi s vankomicinom pripravljeni su metodom hidratacije filma te metodom smrzavanja. Karakterizirani su koristeći optičku mikroskopiju, mikroskopiju atomskih sila (AFM), diferencijalnu skenirajuću kalorimetriju (DSC), dinamičko raspršenje svjetlosti (DLS) te elektokinetiku. Optičkom mikroskopijom dokazano je postojanje liposoma. DSC-om je pokazano kako metode priprave značajno utječu na termička svojstva liposoma. DLS-om je pokazano kako prazni liposomi imaju sličnu raspodjelu u veličini kao i liposomi s uklopljenim vankomicinom. ζ-potencijal praznih liposoma veći je od ζ-potencijala liposoma s uklopljenim vankomicinom što ukazuje na veću nestabilnost i sklonost agregacije liposoma s vankomicinom, što je potvrđeno i AFM-om. Enkapsulacijska efikasnost vankomicina najveća je u lipsomima pripravljenima metodom smrzavanja mjerena 30 dana nakon priprave.
Abstract (english)
Vancomycin is a glicopeptide antibiotic used for treatment of infections caused by methicillin-resistent Staphylococcus aureus. Nephrotoxicity is observed in up to 40% of cases, depending on the dosis. In formulation with liposomes, lipid vesicles, vancomycin’s concentration in the kidney lowers faster, reducing the nephrotoxicity. Vancomycin liposomes were prepared using thin film hydratation and freeze-thaw method. Characterization was made by optical mycroscopy, atomic force microscopy (AFM), differential scanning calorimetry (DSC), dynamic light scattering (DLS) and electrokinetics. Using optical microscopy, the existence of liposomes was proved. DSC results suggest that thermodynamic properties of liposomes significalntly depend of the preparation method. DLS showed that empty liposomes and vancomycin liposomes have almost the same size distribution. ζ-potential was greater for empty liposomes in comparison to vancomycin liposomes, suggesting that vancomycin lipsomes are less stable and more prone to aggregation, which was confirmed by AFM. Encapsulation efficiency of vancomycin was the highest in liposomes prepared by the freez-thaw method when the analysis was performed 30 days after preparation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:365876
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-03-15 14:18:04