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master's thesis
Poništavanje hepatoprotektivnog učinka D-met-enkefalina komplementarnim peptidom kao model za proučavanje peptidnih interakcija
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:811245
Matković, Katarina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Matković, K. (2021). Poništavanje hepatoprotektivnog učinka D-met-enkefalina komplementarnim peptidom kao model za proučavanje peptidnih interakcija (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:811245

Matković, Katarina. "Poništavanje hepatoprotektivnog učinka D-met-enkefalina komplementarnim peptidom kao model za proučavanje peptidnih interakcija." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:811245

Matković, Katarina. "Poništavanje hepatoprotektivnog učinka D-met-enkefalina komplementarnim peptidom kao model za proučavanje peptidnih interakcija." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:811245

Matković, K. (2021). 'Poništavanje hepatoprotektivnog učinka D-met-enkefalina komplementarnim peptidom kao model za proučavanje peptidnih interakcija', Master's thesis, University of Zagreb, Faculty of Science, accessed 16 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:811245

Matković K. Poništavanje hepatoprotektivnog učinka D-met-enkefalina komplementarnim peptidom kao model za proučavanje peptidnih interakcija [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 May 16] Available at: https://urn.nsk.hr/urn:nbn:hr:217:811245

K. Matković, "Poništavanje hepatoprotektivnog učinka D-met-enkefalina komplementarnim peptidom kao model za proučavanje peptidnih interakcija", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:811245

Metadata
TitlePoništavanje hepatoprotektivnog učinka D-met-enkefalina komplementarnim peptidom kao model za proučavanje peptidnih interakcija
Title (english)Reversal of the hepatoprotective effect of D-met-enkephalin by a complementary peptide as a model for studying peptide interactions
AuthorKatarina Matković
MentorPaško Konjevoda (mentor)
MentorRosa Karlić (sumentor)
Committee memberRosa Karlić (predsjednik povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-02-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Većina regulatornih procesa u biološkim sustavima ovisi o interakcijama između proteina. Naše spoznaje o takvim interakcijama su relativno ograničene, a eksperimentalne metode za njihovo proučavanje kompleksne, spore i skupe. Zbog toga se u radu opisuju heurističke metode za određivanje bioaktivnih područja u proteinima te interakcije tih područja s drugim proteinima. Jedna od takvih metoda, teorija molekularnog prepoznavanja (MRT) praktično je primijenjena u biološkom eksperimentalnom modelu poništenja učinka peptida D-met-enkefalina. Protektivni učinak D-met-enkefalina proučavan je u in vivo modelu oštećenja jetre mužjaka miša CBA soja paracetamolom uz primjenu tri kriterija: aktivnost enzima ALT i AST, patohistološko određivanje stupnja nekroze jetre te broj spontano uginulih životinja. Dokazan je protektivni učinak po dva kriterija: aktivnost enzima ALT (U/L) i AST (U/L) te bodovi nekroze jetre. Optimalna protektivna doza iznosi 5 mg/kg. Protektivni učinak poništavao se uz pomoć komplementarnog peptida (IPPLY) konstruiranog uporabom MRT. Osnovna svojstva komplementarnog peptida te njegova interakcija s D-met enkefalinom proučavana su pomoću cirkularne dikroizmatske spektroskopije i fluorescentne spektroskopije. Antisens peptid u potpunosti poništava protektivni učinak optimalne doze D-metenkefalina. Dokazano je da je uporabom teorije molekularnoga prepoznavanja moguće na jednostavan i učinkovit način izraditi peptidni sustav liganda i akceptora.
Abstract (english)
Most regulatory processes in biological systems depend on protein-protein interactions. Our knowledge of such interactions is relatively limited, and experimental methods for studying them are complex, slow and expensive. Therefore, heuristic methods to determine bioactive regions in proteins and interactions of these regions with other proteins are described in the paper. One such method, Molecular Recognition Theory (MRT), has been practically applied in the biological experimental model of reversal effect of the D-met-enkephalin peptide. Protective potential of Metenkephalin was evaluated using an experimental model of paracetamol induced hepatotoxicity in male CBA mice and observed by using three criteria: plasma activities of ALT and AST enzymes, liver necrosis score and number of spontaneously dead animals. Protective potential was demonstrated by two criteria: activity of enzymes ALT (U / L) and AST (U / L) and liver necrosis score. The optimal protective dose is 5 mg / kg. Protective effect was reversed using a complementary peptide (IPPLY) constructed using MRT. The basic properties of the complementary peptide and its interaction with D-met enkephalin were studied using circular dichroism spectroscopy and fluorescence spectroscopy. The antisense peptide completely reverses the protective effect of the optimal dose of D-met-enkephalin. It has been proven that using molecular recognition theory it is possible to construct a ligand-acceptor peptide system in a simple and efficient way.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:811245
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-02-26
Terms of useLicence In copyright icon
Created on2021-03-16 12:17:17