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master's thesis
Istraživanje interakcija pirenskog derivata poliamina spektroskopijom površinski pojačanog Ramanova raspršenja
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:617640
Hudika, Petra
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Hudika, P. (2021). Istraživanje interakcija pirenskog derivata poliamina spektroskopijom površinski pojačanog Ramanova raspršenja (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:617640

Hudika, Petra. "Istraživanje interakcija pirenskog derivata poliamina spektroskopijom površinski pojačanog Ramanova raspršenja." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:617640

Hudika, Petra. "Istraživanje interakcija pirenskog derivata poliamina spektroskopijom površinski pojačanog Ramanova raspršenja." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:617640

Hudika, P. (2021). 'Istraživanje interakcija pirenskog derivata poliamina spektroskopijom površinski pojačanog Ramanova raspršenja', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:617640

Hudika P. Istraživanje interakcija pirenskog derivata poliamina spektroskopijom površinski pojačanog Ramanova raspršenja [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:617640

P. Hudika, "Istraživanje interakcija pirenskog derivata poliamina spektroskopijom površinski pojačanog Ramanova raspršenja", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:617640

Metadata
TitleIstraživanje interakcija pirenskog derivata poliamina spektroskopijom površinski pojačanog Ramanova raspršenja
Title (english)Surface-enhanced Raman study of the interactions between pyrene derivative of polyamine and double-stranded polynucleotide
AuthorPetra Hudika
MentorAdriana Kenđel (mentor)
Committee memberAdriana Kenđel (predsjednik povjerenstva)
Committee memberBiserka Prugovečki (član povjerenstva)
Committee memberIvana Biljan (član povjerenstva)
Committee memberSnežana Miljanić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-02-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Spektroskopijom površinski pojačanog Ramanova raspršenja (SERS) istraţivane su interakcije N,N,N-tri(8-piren-1-il-[3,7-diazaokt-1-il])amina (spoj 1) s polinukleotidima. Kao metalni supstrati korišteni su svjeţe pripravljeni, koncentrirani i agregirani koloid. Spoj 1 najintenzivnije raspršuje zračenje pri koncentraciji 5×10−6 mol dm−3 u koncentriranom koloidu te je navedeni supstrat korišten pri pripravi smjesa s polinukleotidima. Smjese su pripremane na dva načina; kod prvog načina u koloid je dodan istraţivani spoj, a zatim odabrani polinukleotid, dok se kod drugog načina prvo pripremio kompleks spoja 1 i polinukleotida, koji je zatim dodan u koloidnu suspenziju. Dobiveni SERS spektri svih smjesa nisu sadrţavali nove vrpce koje bi odgovarale vibracijama polinukleotida. Adsorpcija molekula spoja 1 na nanočestice je prilično jaka i dodatak polinukleotida slabo utječe na intenzitet i poloţaj vrpca. Kompleks spoj 1/polinukleotid daje slabe vrpce poliaminskog derivata čak i pri velikom suvišku polinukleotida, vrlo vjerojatno zbog interkaliranja pirenskog sustava izmeĎu parova baza DNA analoga, dok nevezani dio molekule spoja 1 ostaje blizu metalne površine.
Abstract (english)
Surface-enhanced Raman scattering (SERS) was used for studying interactions between pyrene derivative of polyamine (1) and polynucleotides. Freshly prepared, concentrated and aggregated colloids were used as metal substrates. Compound 1 scattered radiation most intensively at concentration of 5 × 10−6 mol dm−3 in concentrated colloid, which was therefore used in the preparation of mixtures with polynucleotides. The mixtures were prepared in two ways; firstly, 1 was added to the colloid, followed by adding selected polynucleotide, while in the second method 1/polynucleotide complex was prepared, and then mixed with the colloid. The obtained SERS spectra of all mixtures showed lack of polynucleotides bands. The polyamine adsorption on the silver was quite strong and the addition of polynucleotides poorly effected the bands intensity and position. The compound 1/polynucleotide complex yielded weak bands originating from compound 1, even with a large excess of polynucleotides, most likely due to intercalation of pyrene part between base pairs of DNA analoges, while unbonded part of polyamine molecules remain close to metal surface.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:617640
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-02-26
Terms of useLicence In copyright icon
Created on2021-03-18 09:53:23