prikaz prve stranice dokumenta Validacija komercijalnog kompleta SGM+ za umnažanje DNA lančanom reakcijom polimerazom
Access restricted to students and staff of home institution
master's thesis
Validacija komercijalnog kompleta SGM+ za umnažanje DNA lančanom reakcijom polimerazom
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:721146
Rožić, Sara
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Rožić, S. (2013). Validacija komercijalnog kompleta SGM+ za umnažanje DNA lančanom reakcijom polimerazom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:721146

Rožić, Sara. "Validacija komercijalnog kompleta SGM+ za umnažanje DNA lančanom reakcijom polimerazom." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:721146

Rožić, Sara. "Validacija komercijalnog kompleta SGM+ za umnažanje DNA lančanom reakcijom polimerazom." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:721146

Rožić, S. (2013). 'Validacija komercijalnog kompleta SGM+ za umnažanje DNA lančanom reakcijom polimerazom', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:721146

Rožić S. Validacija komercijalnog kompleta SGM+ za umnažanje DNA lančanom reakcijom polimerazom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:721146

S. Rožić, "Validacija komercijalnog kompleta SGM+ za umnažanje DNA lančanom reakcijom polimerazom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:721146

Metadata
TitleValidacija komercijalnog kompleta SGM+ za umnažanje DNA lančanom reakcijom polimerazom
Title (english)Validation of SGM+ PCR amplification kit at low DNA concentration
AuthorSara Rožić
MentorBranka Gršković (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberBranka Gršković (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberIvana Ivančić Baće (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-07-10, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Unutarnja validacija nove metode je obavezan korak prije njezine implementacije u svakodnevni rad laboratorija. AmpFlSTR® SGM Plus® komplet za umnažanje DNA koristi se za analizu spornih tragova i nespornih uzoraka u forenzične svrhe. Cilj ovog rada bio je validacija AmpFlSTR® SGM Plus® kompleta pri niskim koncentracijama DNA od 0,06 ng/μL, 0,04 ng/μL, 0,02 ng/μL i 0,01 ng/μL iz uzoraka AmpFlSTR®DNA 007 pozitivne kontrole, nespornog uzorka brisa stanica bukalne sluznice te forenzičnog uzorka brisa epitelnih stanica s odjevnih predmeta. DNA analizom pozitivne kontrole potpuni DNA profil utvrđen je pri svim analiziranim razrjeđenjima. Isto je utvrđeno za nesporni uzorak, osim koncentracije 0,01ng/μL. Potpuni DNA profil dobiven DNA analizom forenzičnog uzorka utvrđen je samo pri koncentraciji 0,06 ng/μL, a u ostalim koncentracijama dobiveni su djelomični DNA profili.
Abstract (english)
Internal validation of a new method is mandatory step before its implementation into the everyday casework. AmpFlSTR® SGM Plus® Amplification Kit is used for the analysis of traces and reference samples for forensic purposes. The aim of this study was to validate AmpFlSTR® SGM Plus® Amplification Kit at low DNA concentrations as follows: 0.06 ng/μL, 0.04 ng/μL, 0.02 ng/μL and 0.01 ng/μL from AmpFlSTR® DNA positive control, reference buccal swab sample and forensic epithelial cells sample. Full DNA profile was detected in positive DNA control in all dilutions. Also, the same was observed for reference sample except in concentration 0.01 ng/μL. Full DNA profile was found only at concentration 0.06 ng/μL in forensic sample. In addition, a partial DNA profiles were observed in all other concentrations tested. (81 pages, 48 figures, 11 tables, 31 references, original in: croatian)
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:721146
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 08:00:23