prikaz prve stranice dokumenta Uloga polimorfizama gena KRAS na mjestima vezanja mikro RNA u tumorigenezi sporadičnih karcinoma debeloga crijeva
  Download
PDF 2.22 MB
doctoral thesis
Uloga polimorfizama gena KRAS na mjestima vezanja mikro RNA u tumorigenezi sporadičnih karcinoma debeloga crijeva
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:148023
Poljak, Mirko
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Poljak, M. (2021). Uloga polimorfizama gena KRAS na mjestima vezanja mikro RNA u tumorigenezi sporadičnih karcinoma debeloga crijeva (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:148023

Poljak, Mirko. "Uloga polimorfizama gena KRAS na mjestima vezanja mikro RNA u tumorigenezi sporadičnih karcinoma debeloga crijeva." Doctoral thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:148023

Poljak, Mirko. "Uloga polimorfizama gena KRAS na mjestima vezanja mikro RNA u tumorigenezi sporadičnih karcinoma debeloga crijeva." Doctoral thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:148023

Poljak, M. (2021). 'Uloga polimorfizama gena KRAS na mjestima vezanja mikro RNA u tumorigenezi sporadičnih karcinoma debeloga crijeva', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:148023

Poljak M. Uloga polimorfizama gena KRAS na mjestima vezanja mikro RNA u tumorigenezi sporadičnih karcinoma debeloga crijeva [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:148023

M. Poljak, "Uloga polimorfizama gena KRAS na mjestima vezanja mikro RNA u tumorigenezi sporadičnih karcinoma debeloga crijeva", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:148023

Metadata
TitleUloga polimorfizama gena KRAS na mjestima vezanja mikro RNA u tumorigenezi sporadičnih karcinoma debeloga crijeva
Title (english)The role of KRAS polymorphisms in micro RNA binding sites in sporadic colon cancer tumorigenesis
AuthorMirko Poljak
MentorSanja Kapitanović (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberMaja Sirotković-Skerlev (član povjerenstva)
Committee memberAnita Škrtić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-03-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Mutacija gena KRAS jedna je od ključnih promjena u tumorigenezi karcinoma debelog crijeva, međutim i pojačana ekspresija proteina KRAS može imati sličan učinak na proliferaciju tumorskih stanica. Cilj ovog istraživanja bio je ispitati ulogu let-7a i miR-544 te polimorfizama gena KRAS na mjestima vezanja za mikro RNA u tumorigenezi sporadičnih karcinoma kolona bez mutacije gena KRAS. Od svih tumora bez mutacije gena KRAS 62,0 % je bilo pozitivno na protein KRAS te je dokazana značajna razlika u ekspresiji između središta tumora i invazivne fronte tumora kako u postotku pozitivnih stanica tako i u razini ekspresije proteina KRAS. Nije dokazana razlika u ekspresiji mRNA KRAS i let-7a između normalnog i tumorskog tkiva, međutim ekspresija miR-544 je bila značajno niža u uzorcima invazivne fronte tumora. U korelaciji s ekspresijom proteina KRAS ekspresija miR-544 bila je značajno snižena u uzorcima invazivne fronte tumora pozitivnih na protein KRAS. Dokazana je povezanost polimorfizma let-7a, ali ne i polimorfizma miR-544 i ekspresije proteina KRAS. Naši rezultati sugeriraju povezanost polimorfizma KRAS-LCS6 i ekspresije miR-544 u regulaciji ekspresije proteina KRAS u sporadičnim tumorima debelog crijeva negativnim na mutaciju gena KRAS.
Abstract (english)
KRAS is one of the key players in CRC tumorigenesis. Although it is known that KRAS mutations are a dominant event in CRC tumorigenesis, KRAS overexpression can have a similar effect on cell proliferation and can be regulated by miRNAs. The aim of this study was to examine the role of let-7a and miR-544 and KRAS polymorphisms in microRNA binding sites in sporadic colon cancer negative to KRAS mutations. In tumors negative to KRAS mutations 62.0 % were positive for KRAS protein expression and there was a significant difference between tumor centre and invasive tumor front in the percentage of positive tumor cells as well as in the intensity of KRAS protein expression. Even though there was no diffrerence in KRAS mRNA and let-7a expression between normal and tumor tissues, expression of miR-544 was significantly decreased in invasive tumor front. When correlated with protein expression, miR-544 was significantly lower in invasive tumor front, but only in tumors positive to KRAS protein. Association was found for let-7a polymorphism and KRAS protein expression but not for miR-544 polymorphism. Our results suggest the role of KRAS-LCS6 polymorphism and miR-544 expression in regulation of KRAS protein expression in sporadic colon cancers negative to KRAS mutations.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:148023
Promotion2021
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-03-24
Terms of useLicence In copyright icon
Created on2021-04-13 08:17:30