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master's thesis
Razina izraženosti transkripcijskogfaktora LEF-1 u glioblastomima
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:334366
Sukser, Viktorija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Sukser, V. (2013). Razina izraženosti transkripcijskogfaktora LEF-1 u glioblastomima (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:334366

Sukser, Viktorija. "Razina izraženosti transkripcijskogfaktora LEF-1 u glioblastomima." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:334366

Sukser, Viktorija. "Razina izraženosti transkripcijskogfaktora LEF-1 u glioblastomima." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:334366

Sukser, V. (2013). 'Razina izraženosti transkripcijskogfaktora LEF-1 u glioblastomima', Master's thesis, University of Zagreb, Faculty of Science, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:334366

Sukser V. Razina izraženosti transkripcijskogfaktora LEF-1 u glioblastomima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:334366

V. Sukser, "Razina izraženosti transkripcijskogfaktora LEF-1 u glioblastomima", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:334366

Metadata
TitleRazina izraženosti transkripcijskogfaktora LEF-1 u glioblastomima
Title (english)ANALYSIS OF THE LEVEL OF EXPRESSION OF TRANSCRIPTION FACTOR LEF-1 IN GLIOBLASTOMAS
AuthorViktorija Sukser
MentorNives Pećina-Šlaus (mentor)
MentorDubravka Hranilović (sumentor)
Committee memberDubravka Hranilović (predsjednik povjerenstva)
Committee memberNives Pećina-Šlaus (član povjerenstva)
Committee memberGordana Lacković-Venturin (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-09-04, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Glioblastomi su najčešći i najagresivniji tumori mozga odraslih osoba, te se prema kriterijima Svjetske zdravstvene organizacije klasificiraju kao astrocitomi gradusa IV. Mogu nastati de novo (primarni) ili iz astrocitoma nižeg gradusa (sekundarni). Svaki posjeduje karakteristične genetičke promjene. Među signalnim putevima koje pogađaju ovi poremećaji nalazi se put Wnt, važan tijekom normalnog embrionalnog razvoja i u matičnim stanicama. U njemu je ključna molekula β-katenin: kada nema signala on se razgrađuje, a kada je put Wnt aktivan, tada β-katenin ulazi u jezgru i veže se za transkripcijske faktore iz obitelji TCF/LEF. Jedan od njenih članova je LEF-1 (lymphoid enhancer factor-1) – zajedno s β-kateninom pokreće transkripciju mnogih gena koji, ukoliko je signalizacija nepravilna, doprinose napretku i invazivnosti tumora. U ovom radu analizirana je razina izraženosti LEF-1 u uzorcima tkiva glioblastoma. Imunohistokemijskim metodama protein LEF-1 lokaliziran je u jezgrama stanica te je utvrđeno da je njegova ekspresija pojačana u 80,6% analiziranih uzoraka.
Abstract (english)
Glioblastomas are the most common and most aggressive brain tumours in adults, and are classified as grade IV astrocytoma by the criteria of the World Health Organization. They can develop either de novo (primary) or from a lower-grade astrocytoma (secondary). Each type has its own characteristic genetic changes. Among the signalling pathways affected by these disorders is the Wnt pathway, which is important during normal embryonic development, as well as in stem cells. The key molecule in this pathway is β-catenin: when signals are absent, it is degraded, and when the Wnt pathway is activated, then β-catenin enters the nucleus and binds to transcription factors of the TCF/LEF family. One of its members is LEF-1 (lymphoid enhancer factor-1) which, together with β-catenin, drives the transcription of many genes that, if the signalling is aberrant, contribute to progression and invasiveness of the tumour. This study analyses the level of expression of LEF-1 in glioblastoma tissue samples. With the use of immunohistochemical methods, protein LEF-1 was localized in the cells' nuclei and its expression was found to be elevated in 80,6% of analyzed samples.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:334366
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 08:25:42