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master's thesis
Ekotoksikološka procjena utjecaja kardiovaskularnih farmaceutika prisutnih u vodenim ekosustavima na embrije ribe zebrice Danio rerio (Hamilton, 1822)
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:948364
Prgić, Gabrijela
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Prgić, G. (2021). Ekotoksikološka procjena utjecaja kardiovaskularnih farmaceutika prisutnih u vodenim ekosustavima na embrije ribe zebrice Danio rerio (Hamilton, 1822) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:948364

Prgić, Gabrijela. "Ekotoksikološka procjena utjecaja kardiovaskularnih farmaceutika prisutnih u vodenim ekosustavima na embrije ribe zebrice Danio rerio (Hamilton, 1822)." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:948364

Prgić, Gabrijela. "Ekotoksikološka procjena utjecaja kardiovaskularnih farmaceutika prisutnih u vodenim ekosustavima na embrije ribe zebrice Danio rerio (Hamilton, 1822)." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:948364

Prgić, G. (2021). 'Ekotoksikološka procjena utjecaja kardiovaskularnih farmaceutika prisutnih u vodenim ekosustavima na embrije ribe zebrice Danio rerio (Hamilton, 1822)', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 July 2024, https://urn.nsk.hr/urn:nbn:hr:217:948364

Prgić G. Ekotoksikološka procjena utjecaja kardiovaskularnih farmaceutika prisutnih u vodenim ekosustavima na embrije ribe zebrice Danio rerio (Hamilton, 1822) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 July 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:948364

G. Prgić, "Ekotoksikološka procjena utjecaja kardiovaskularnih farmaceutika prisutnih u vodenim ekosustavima na embrije ribe zebrice Danio rerio (Hamilton, 1822)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:948364

Metadata
TitleEkotoksikološka procjena utjecaja kardiovaskularnih farmaceutika prisutnih u vodenim ekosustavima na embrije ribe zebrice Danio rerio (Hamilton, 1822)
Title (english)Ecotoxicological assessment of the impact of cardiovascular pharmaceuticals present in aquatic ecosystems on zebrafish embryos Danio rerio (Hamilton, 1822)
AuthorGabrijela Prgić
MentorGoran Klobučar (mentor)
MentorSanja Babić (mentor)
Committee memberGoran Klobučar (predsjednik povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberPetar Kružić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-06-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Proces onečišćenja okoliša doživljava eksponencijalan i nezaustavljiv rast te je postao jedan od najvećih problema s kojima se danas čovječanstvo susreće. Napredak znanosti doveo je i novu vrstu onečišćenja, lijekovima, odnosno farmaceutski aktivnim spojevima koji su jedna od glavnih komponenti onečišćenja kopnenih voda i koji su utvrđeni u sedimentu i plazmi riba iz rijeke Save. Na temelju dosadašnjih istraživanja izdvojeni su kardiovaskularni lijekovi: amiodaron, ramipril, simvastatin i verapamil čiji je utjecaj istražen, pojedinačno i u smjesi, na razvoj embrija ribe zebrice Danio rerio (Hamilton, 1822) u prvih 96 hpf. Tijekom izlaganja okolišno relevantnim koncentracijama (0.1, 1.0 i 10.0 μg/L) praćene su izazvane promjene kao što su mortalitet i stopa abnormalnosti, dužina ličinki, stopa izvaljivanja, brzina otkucaja srca, spontani pokreti prsnim perajama i oksidativni stres. Povećanjem koncentracije i vremenske izloženosti jedinki testiranim spojevima uočeni su učinci pri izlaganju svim spojevima, no najveća toksičnost zabilježena je na uzorku simvastatina. Integracijom krajnjih bioloških učinaka ispitivanih unutar ovog istraživanja, potvrđena je izvrsna uzročno-posljedična veza između izlaganja farmaceutskim spojevima i zabilježenog učinka na akvatičkom organizmu, čime je pružena podloga za biomonitoring onečišćenja okoliša, posebice slatkovodnih ekosustava.
Abstract (english)
The process of environmental pollution is experiencing exponential and unstoppable growth and has become one of the biggest problems facing humanity today. Progress in science has also brought a new type of pollution, therapeutic drugs, or pharmaceutically active compounds that are one of the main components of inland water pollution and that have been found in the sediment and plasma of fish from the Sava River. Based on previous research, cardiovascular drugs have been chosen: amiodarone, ramipril, simvastatin and verapamil, the effect of which has been investigated, individually and in a mixture, on the development of the zebrafish embryo Danio rerio (Hamilton, 1822) in the first 96 hpf. During exposure to environmentally relevant concentrations (0.1, 1.0 and 10.0 μg/L), caused changes such as mortality and abnormality rate, larval length, hatching rate, heart rate, spontaneous movements of the pectoral fins and oxidative stress were monitored. With increasing concentration and time exposure of individuals to the tested compounds, effects on exposure to all compounds were observed, but the highest toxicity was observed on the simvastatin sample. The integration of the ultimate biological effects examined within this study confirmed the excellent cause-and-effect relationship between exposure to pharmaceutical compounds and the observed effect on the aquatic organism, thus providing a basis for biomonitoring of environmental pollution, especially freshwater ecosystems.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:948364
Study programmeTitle: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-06-17
Terms of useLicence In copyright icon
Created on2021-06-23 14:09:45