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master's thesis
Ekspresija proteina neuroplastina i kalcijske ATP-aze stanične membrane u mišjem modelu neurodegeneracije
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:962347
Oršulić, Antonia
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Oršulić, A. (2021). Ekspresija proteina neuroplastina i kalcijske ATP-aze stanične membrane u mišjem modelu neurodegeneracije (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:962347

Oršulić, Antonia. "Ekspresija proteina neuroplastina i kalcijske ATP-aze stanične membrane u mišjem modelu neurodegeneracije." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:962347

Oršulić, Antonia. "Ekspresija proteina neuroplastina i kalcijske ATP-aze stanične membrane u mišjem modelu neurodegeneracije." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:962347

Oršulić, A. (2021). 'Ekspresija proteina neuroplastina i kalcijske ATP-aze stanične membrane u mišjem modelu neurodegeneracije', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:962347

Oršulić A. Ekspresija proteina neuroplastina i kalcijske ATP-aze stanične membrane u mišjem modelu neurodegeneracije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:962347

A. Oršulić, "Ekspresija proteina neuroplastina i kalcijske ATP-aze stanične membrane u mišjem modelu neurodegeneracije", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:962347

Metadata
TitleEkspresija proteina neuroplastina i kalcijske ATP-aze stanične membrane u mišjem modelu neurodegeneracije
Title (english)Protein neuroplastin and plasma membrane calcium ATP-ase expression in a mouse model of neurodegeneration
AuthorAntonia Oršulić
MentorKatarina Ilić (mentor)
MentorDubravka Hranilović (sumentor)
Committee memberDubravka Hranilović (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-07-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Neuroplastini imaju ulogu u rastu neurita, modulaciji dugoročne sinaptičke plastičnosti, ekscitaciji neurona te regulaciji kalcijske ATP-aze stanične membrane PMCA (od engl. Plasma membrane calcium ATP-ase) i posljedično razine unutarstaničnog kalcija. Promjena ekspresije neuroplastina i s njim povezane PMCA, dovode do poremećene stanične signalizacije ovisne o kalciju čime se narušava razvoj mreţe neurona, smanjuje se oslobađanje neurotransmitera i nemogućnosti odrţavanja molekularnih mehanizama odgovornih za učenje i pamćenje što moţe biti podloga neurodegenerativnih promjena u Alzheimerovoj bolesti. U ovom diplomskom radu istraţeno je dolazi li do promjene ekspresije neuroplastina i PMCA u neurodegeneraciji u različitim regijama mozga u dvije starosne skupine miševa. Na tkivu mozgova miševa divljeg tipa i 5xFAD, modela za obiteljsku Alzheimerovu bolest, imunohistokemijski je određena lokalizacija i analizirana ekspresija oba proteina u tri moţdane regije: hipokampusu, malome mozgu i moţdanoj kori, te je kvantificirana razlika u ekspresiji. Preklapanjem imunofluorescentnih signala pokazana je kolokalizacija neuroplastina i PMCA u svim regijama mozga što upućuje na povezanost neuroplastina s ekspresijom i membranskim pozicioniranjem PMCA. Ekspresija neuroplastina i PMCA u modelu 5xFAD je manji u kori velikog mozga i hipokampusu u odnosu na divlji tip što moţe biti molekularna osnova za poremećaj procesa pamćenja.
Abstract (english)
Neuroplastins play a role in neurite outgrowth, modulation of long-term synaptic plasticity, neuronal excitation, regulation of plasma membrane calcium ATPase (PMCA) and consequently intracellular calcium levels. Alterations in neuroplastin and PMCA expression lead to impaired calcium-dependent cellular signaling and consequently to impaired neuronal network development, decreased neurotransmitter release, and the inability to sustain the molecular mechanisms responsible for learning and memory which could underlie the neurodegenerative mechanisms of Alzheimer's disease. In this work, we investigated whether there is a change in neuroplastin and PMCA expression in neurodegeneration in different brain regions and two age groups of mice. In brain tissue from wild-type mice and 5xFAD, a model for familial Alzheimer's disease, immunohistochemistry was used to determine the localization and expression of both proteins in three brain regions: hippocampus, cerebellum and cerebral cortex, and the difference in expression was quantified. The overlap of immunofluorescence signals showed the colocalization of neuroplastin and PMCA in all brain regions, indicating the association of neuroplastin with the expression and membrane positioning of PMCA. The expression of neuroplastin and PMCA in the 5xFAD model is lower in the cerebral cortex and hippocampus compared to wild type, which may be the molecular basis for the disruption of the memory process.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:962347
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-07-06
Terms of useLicence In copyright icon
Created on2021-07-22 10:07:23