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master's thesis
Potencijalno neuroprotektivno djelovanje dehidroepiandrosterona (DHEA) i njegovog sulfata (DHEAS) u modelu ozljede mozga
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:507952
Blažanović, Suzana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Blažanović, S. (2021). Potencijalno neuroprotektivno djelovanje dehidroepiandrosterona (DHEA) i njegovog sulfata (DHEAS) u modelu ozljede mozga (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:507952

Blažanović, Suzana. "Potencijalno neuroprotektivno djelovanje dehidroepiandrosterona (DHEA) i njegovog sulfata (DHEAS) u modelu ozljede mozga." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:507952

Blažanović, Suzana. "Potencijalno neuroprotektivno djelovanje dehidroepiandrosterona (DHEA) i njegovog sulfata (DHEAS) u modelu ozljede mozga." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:507952

Blažanović, S. (2021). 'Potencijalno neuroprotektivno djelovanje dehidroepiandrosterona (DHEA) i njegovog sulfata (DHEAS) u modelu ozljede mozga', Master's thesis, University of Zagreb, Faculty of Science, accessed 05 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:507952

Blažanović S. Potencijalno neuroprotektivno djelovanje dehidroepiandrosterona (DHEA) i njegovog sulfata (DHEAS) u modelu ozljede mozga [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 May 05] Available at: https://urn.nsk.hr/urn:nbn:hr:217:507952

S. Blažanović, "Potencijalno neuroprotektivno djelovanje dehidroepiandrosterona (DHEA) i njegovog sulfata (DHEAS) u modelu ozljede mozga", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:507952

Metadata
TitlePotencijalno neuroprotektivno djelovanje dehidroepiandrosterona (DHEA) i njegovog sulfata (DHEAS) u modelu ozljede mozga
Title (english)Potential neuroprotective effects of dehydroepiandsterone (DHEA) and its sulphate (DHEAS) in brain injury model
AuthorSuzana Blažanović
MentorMatea Nikolac Perković (mentor)
MentorSofia Ana Blažević (sumentor)
Committee memberSofia Ana Blažević (predsjednik povjerenstva)
Committee memberInga Urlić (član povjerenstva)
Committee memberAntun Alegro (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Neurosteroid dehidroepiandrosteron (DHEA) ključan je u modulaciji neurogeneze, metabolizma neurona i njihovog preživljenja, a uključen je i u regulaciju važnih moždanih funkcija poput neuralne plastičnosti, učenja, pamćenja i ponašanja. Dosadašnja istraživanja pokazala su da DHEA i njegov sulfat (DHEAS) ublažavaju posljedice ekscitotoksičnosti i oštećenja prouzrokovanih oksidativnim stresom te imaju potencijalno protektivno djelovanje u slučaju ishemijske ozljede mozga. Deprivacija glukoze, smanjen protok krvi te hipometabolizam glukoze povezuju se s degeneracijom i smrti neurona kod ishemijskog moždanog udara, ali i kod neurodegenerativnih bolesti kao što su Alzheimerova te Parkinsonova bolest. Sve navedeno upućuje na to da bi DHEA i DHEAS mogli biti obećavajući kandidati za istraživanja usmjerena prema zaštiti neurona, odnosno prema smanjenju oštećenja i odumiranja neurona koji prate različita akutna stanja, kronične neurodegenerativne bolesti te normalno starenje. Cilj ovog diplomskog rada je istražiti i razjasniti potencijalne učinke neurosteroida DHEA i DHEAS u prevenciji neuronske smrti i promicanju preživljavanja neurona uporabom in vitro pristupa, odnosno uporabom modela deprivacije kisika i glukoze, te reperfuzije (OGD/R) koji vrlo dobro oponaša ishemijske uvjete i s tim povezana patološka stanja. Rezultati istraživanja upućuju na to da ispitivani neurosteroidi imaju potencijal u liječenju i prevenciji ozljeda izazvanih ishemijom, kao i onih uzrokovanih neurodegeneracijom.
Abstract (english)
The neurosteroid dehydroepiandrosterone (DHEA) is crucial in modulating neurogenesis, neuronal metabolism, and survival, and is involved in the regulation of important brain functions such as neural plasticity, learning, memory, and behavior. Previous research has shown that DHEA and its sulfate (DHEAS) alleviate the effects of excitotoxicity and damage caused by oxidative stress and have potentially protective effects in the case of ischemic brain injury. Glucose deprivation, decreased blood flow and glucose hypometabolism are associated with degeneration and death of neurons in ischemic stroke, but also in neurodegenerative diseases such as Alzheimer's and Parkinson's disease. All of the above suggests that DHEA and DHEAS could be promising candidates for research aimed at protecting neurons, i.e. reducing the damage and death of neurons that accompany various acute conditions, chronic neurodegenerative diseases and normal aging. The aim of this thesis is to investigate and clarify the potential effects of the neurosteroid DHEA and DHEAS in preventing neuronal death and promoting neuronal survival using an in vitro approach, i.e. using a model of oxygen and glucose deprivation and reperfusion (OGD/R) that mimics very well ischemic conditions and related pathological conditions. Pretreatment and posttreatment with DHEA (S) proved to be neuroprotective. The results of the study suggest that the studied neurosteroids have potential in the treatment and prevention of injuries caused by ischemia, as well as those caused by neurodegeneration.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:507952
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-07-23 10:33:38