prikaz prve stranice dokumenta Uloga izoformi plektina u organizaciji acetilkolinskih receptora i funkciji neuromuskularne veze
Access restricted to students and staff of home institution
master's thesis
Uloga izoformi plektina u organizaciji acetilkolinskih receptora i funkciji neuromuskularne veze
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:024235
Žarak, Martina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Žarak, M. (2013). Uloga izoformi plektina u organizaciji acetilkolinskih receptora i funkciji neuromuskularne veze (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:024235

Žarak, Martina. "Uloga izoformi plektina u organizaciji acetilkolinskih receptora i funkciji neuromuskularne veze." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:024235

Žarak, Martina. "Uloga izoformi plektina u organizaciji acetilkolinskih receptora i funkciji neuromuskularne veze." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:024235

Žarak, M. (2013). 'Uloga izoformi plektina u organizaciji acetilkolinskih receptora i funkciji neuromuskularne veze', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:024235

Žarak M. Uloga izoformi plektina u organizaciji acetilkolinskih receptora i funkciji neuromuskularne veze [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:024235

M. Žarak, "Uloga izoformi plektina u organizaciji acetilkolinskih receptora i funkciji neuromuskularne veze", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:024235

Metadata
TitleUloga izoformi plektina u organizaciji acetilkolinskih receptora i funkciji neuromuskularne veze
Title (english)Plectin isoform specific role in acetylocholin receptor clustering and function of neuromuscular junction
AuthorMartina Žarak
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-10-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Plektin je citolinker protein koji ima važnu ulogu u funkciji stanice te je odgovoran za otpornost stanica na mehanički stres. Nedostatak plektina rezultira razvojem bulozne epidermiolize koja je u nekim slučajevima povezana s mišićnom distrofijom (EBD-MD) u kombinaciji s mijasteničnim sindromom (MyS), a koju karakterizira poremećaj u prijenosu signala na području neuromuskularne veze (NMV). Formiranje gustih nakupina AChR na postsinaptičkom dijelu NMV je ključno za učinkovit prijenos signala s motoričkih neurona na skeletne mišiće te je uloga plektina u ovom procesu predmet istraživanja ovog rada. Glavni cilj ovog istraživanja bio je utvrđivanje uloge izoformi plektina u organizaciji AChR te identifikacija specifičnih interacijskih partnera odgovornih za usmjeravanje plektina prema AChR kompleksu putem analize imunofluorescencije te in vitro testova vezivanja. Uloga plektina u organizaciji AChR je potvrđena, te je utvrđena specifičnost veze između izoforme plektina 1f i rapsina putem ABD plektina, kao i interakcija s intergrinom β1 te β-distroglikanom.
Abstract (english)
Plectin is a cytolinker protein important for vital cellular functions as well as mechanical resistance. Plectin deficiency results in epidermolysis bullosa simplex which in some patients is associated with muscular dystrophy (EBS-MD) and a severe myasthenic syndrome (MyS), characterized by impaired signal transmission at neuromuscular junctions (NMJs). For effective signal transmission from motor neurons to skeletal muscles, formation of dense AChR patches at the postsynaptic part of NMJs is of outmost importance and plectin’s involvement in this process was investigated in this thesis. The major goal of this project was the discovery of plectin isoform specific role in clustering of AChR and specific interaction partner(s) responsible for plectin’s targeting towards the AChR complex using immunofluorescence analysis and in vitro binding assays. The role of plectin in AChR clustering has been confirmed and specificity of the interaction between plectin 1f isoform and rapsyn via plectin’s ABD has been established, as well as with intergrin β1 and β-dystroglycan.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:024235
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 09:02:39