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doctoral thesis
Utjecaj spolnih hormona na N-glikozilaciju imunoglobulina G u žena
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:579124
Jurić, Julija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jurić, J. (2021). Utjecaj spolnih hormona na N-glikozilaciju imunoglobulina G u žena (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:579124

Jurić, Julija. "Utjecaj spolnih hormona na N-glikozilaciju imunoglobulina G u žena." Doctoral thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:579124

Jurić, Julija. "Utjecaj spolnih hormona na N-glikozilaciju imunoglobulina G u žena." Doctoral thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:579124

Jurić, J. (2021). 'Utjecaj spolnih hormona na N-glikozilaciju imunoglobulina G u žena', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:579124

Jurić J. Utjecaj spolnih hormona na N-glikozilaciju imunoglobulina G u žena [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:579124

J. Jurić, "Utjecaj spolnih hormona na N-glikozilaciju imunoglobulina G u žena", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:579124

Metadata
TitleUtjecaj spolnih hormona na N-glikozilaciju imunoglobulina G u žena
Title (english)Effect of sex hormones on N-glycosylation of immunoglobulin G in women
AuthorJulija Jurić
MentorMarija Pezer (mentor)
Committee memberGordan Lauc (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberAleksandar Vojta (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-06-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
N-glikozilacija je posttranslacijska modifikacija imunoglobulina G (IgG) koja se mijenja u bolestima, sa starenjam i pod utjecajem spolnih hormona. U ovom doktorskom radu tekućinskom kromatografijom ultravisoke učinkovitosti analizirani su glikanski profili protutijela IgG iz krvne plazme zdravih žena tijekom menstruacijskih ciklusa (MC) iz ukupno 12 vremenskih točaka – svakih 7 dana tijekom 3 mjeseca. Primijećene su periodične cikličke promjene N-glikozilacije povezane s fazama ciklusa i koncentracijom spolnih hormona. Estrogen i progesteron su povezani s povećanom razinom digalaktoziliranih i sijaliniziranih N-glikana u luteinskoj fazi, a testosteron je povezan s povećanom razinom agalaktoziliranih i monogalaktoziliranih N-glikana u folikularnoj fazi ciklusa. Najveću varijabilnost unutar osobe imali su sijalinizirani i agalaktozilirani N-glikani, dok se fukozilacija nije mijenjala tijekom MC. Prosječna varijabilnost N-glikozilacije u ciklusima svih ispitanica su vrlo male, do 0.8%, što omogućuje da se prikupljanje i analiza N-glikana protutijela IgG iz uzoraka krvi žena u studijama s velikim brojem ispitanika odvija neovisno o fazi MC.
Abstract (english)
N-glycosylation is a post-translational modification of immunoglobulin G (IgG) that changes in diseases, with ageing and under the influence of sex hormones. In this doctoral dissertation, plasma-derived IgG from 12 time points – every 7 days during 3 months in the menstrual cycles (MC) of 70 healthy women was glycoprofiled by ultra-performance liquid chromatography. Periodic cyclic N-glycosylation changes which associated with MC phases and plasma sex hormone concentrations were observed. Estrogen and progesterone associated with increased digalactosylated and sialylated N-glycans in the luteal phase, while testosterone associated with increased agalactosylated and monogalactosylated N-glycans in the follicular phase of the MC. The highest intra-individual variation was observed for sialylated and agalactosylated N-glycans, whereas fucosylation did not change during the MC. The average N-glycosylation variability in the MC is very small on the cohort level, up to 0.8%, meaning that sample collection and IgG N-glycoprofiling of women in large cohort studies can take place independently of the MC phase.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:579124
Promotion2021
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-07-23 10:47:45