prikaz prve stranice dokumenta Analiza tetrahidrofurfurilnog derivata 3-makrozona i komponenata reakcijske smjese primjenom sustava LC-SPE/NMR
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master's thesis
Analiza tetrahidrofurfurilnog derivata 3-makrozona i komponenata reakcijske smjese primjenom sustava LC-SPE/NMR
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:086883
Sekula, Lucia Ema
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Sekula, L. E. (2021). Analiza tetrahidrofurfurilnog derivata 3-makrozona i komponenata reakcijske smjese primjenom sustava LC-SPE/NMR (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:086883

Sekula, Lucia Ema. "Analiza tetrahidrofurfurilnog derivata 3-makrozona i komponenata reakcijske smjese primjenom sustava LC-SPE/NMR." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:086883

Sekula, Lucia Ema. "Analiza tetrahidrofurfurilnog derivata 3-makrozona i komponenata reakcijske smjese primjenom sustava LC-SPE/NMR." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:086883

Sekula, L. E. (2021). 'Analiza tetrahidrofurfurilnog derivata 3-makrozona i komponenata reakcijske smjese primjenom sustava LC-SPE/NMR', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:086883

Sekula LE. Analiza tetrahidrofurfurilnog derivata 3-makrozona i komponenata reakcijske smjese primjenom sustava LC-SPE/NMR [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:086883

L. E. Sekula, "Analiza tetrahidrofurfurilnog derivata 3-makrozona i komponenata reakcijske smjese primjenom sustava LC-SPE/NMR", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:086883

Metadata
TitleAnaliza tetrahidrofurfurilnog derivata 3-makrozona i komponenata reakcijske smjese primjenom sustava LC-SPE/NMR
Title (english)The analysis of tetrahydrofurfuryl 3-macrozone derivative and reaction mixture components using the LC-SPE/NMR
AuthorLucia Ema Sekula
MentorPredrag Novak (mentor)
Committee memberPredrag Novak (predsjednik povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberMirta Rubčić (član povjerenstva)
Committee memberTomislav Jednačak (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Makrozoni čine novu klasu spojeva nastalih konjugacijom azitromicina i derivata tiosemikarbazona koji su pokazali izvrsno antibakterijsko djelovanje in vitro na odabrane Gram-pozitivne i Gram-negativne bakterije. Velik porast rezistencije bakterija na postojeće antibiotike postao je globalni zdravstveni problem, pa je potreba za razvojem novih antibiotika jedan od glavnih ciljeva suvremene medicinske i farmaceutske kemije. Učinkovito i brzo pročišćavanje te izolacija novosintetiziranih bioaktivnih spojeva važan je korak u procesu sinteze koji prethodi biološkom testiranju novih molekula. Također, izolacija i karakterizacija onečišćenja važan je korak razvoja lijekova u farmaceutskoj industriji. Preparativna i semi-preparativna tekućinska kromatografija su klasične tehnike pročišćavanja spojeva koje iziskuju mnogo vremena i veliku količinu otapala. S ciljem smanjenja trajanja analize i utroška otapala, novosintetizirane spojeve moguće je analizirati vezanim sustavima. U ovom je radu uspješno provedena izolacija i strukturna karakterizacija novosintetiziranog bioaktivnog tetrahidrofurfurilnog derivata 3-makrozona i nekih onečišćenja iz reakcijske smjese primjenom vezanog sustava LC-SPE/NMR. Strukture izoliranih spojeva predložene su na temelju analize jedno- i dvodimenzijskih spektara NMR te spektara MS. U radu su raspravljene glavne prednosti ovog pristupa u usporedbi s klasičnim tehnikama off-line.
Abstract (english)
Macrozones are novel bioactive conjugates of azithromycin and thiosemicarbazones that possess very good in vitro antibacterial activity against selected Gram-positive and Gram-negative bacteria. Due to a global problem of growing bacterial resistance, development of new antibiotics and understanding of resistance mechanisms is of an outmost importance for modern medicine and pharmaceutical chemistry. Efficient and rapid purification and isolation of newly synthesized bioactive compounds is a crucial step prior to biological evaluation. Furthermore, isolation and identification of impurities is important during the drug development process in the pharmaceutical industry. Preparative and semi-preparative liquid chromatography are classical, but time- and solvent-consuming purification methods. Using hyphenated systems enables shorter analysis time and reduces solvent consumption. In this study successful isolation and structural characterization of newly synthesized tetrahydrofurfuryl 3-macrozone derivative and some impurities from reaction mixture have been performed using the LC-SPE/NMR approach. Structures of the isolated compounds were proposed on the basis of the analyzed one- and two-dimensional NMR spectra and MS spectra, respectively. The main advantages of the LC-SPE/NMR approach over classical off-line techniques are discussed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:086883
ProjectNumber: IP-2018-01-8098
Title: Makrozoni, novi konjugati makrolidnih antibiotika: Dizajn, priprava i interakcije
Title: Macrozones, New Conjugates of Macrolide Antibiotics: Design, Synthesis and Interactions
Acronym: Makrozoni/Macrozones
Leader: Predrag Novak
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-08-25 11:42:16