prikaz prve stranice dokumenta Računalna analiza obrazaca poliadenilacije glasničke RNA u genomu kralješnjaka
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master's thesis
Računalna analiza obrazaca poliadenilacije glasničke RNA u genomu kralješnjaka
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:902958
Barilar, Ivan
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Barilar, I. (2012). Računalna analiza obrazaca poliadenilacije glasničke RNA u genomu kralješnjaka (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:902958

Barilar, Ivan. "Računalna analiza obrazaca poliadenilacije glasničke RNA u genomu kralješnjaka." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:902958

Barilar, Ivan. "Računalna analiza obrazaca poliadenilacije glasničke RNA u genomu kralješnjaka." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:902958

Barilar, I. (2012). 'Računalna analiza obrazaca poliadenilacije glasničke RNA u genomu kralješnjaka', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:902958

Barilar I. Računalna analiza obrazaca poliadenilacije glasničke RNA u genomu kralješnjaka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:902958

I. Barilar, "Računalna analiza obrazaca poliadenilacije glasničke RNA u genomu kralješnjaka", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:902958

Metadata
TitleRačunalna analiza obrazaca poliadenilacije glasničke RNA u genomu kralješnjaka
Title (english)Computational analysis of mRNA poliadentilation patterns in vertebrate genomes
AuthorIvan Barilar
MentorKristian Vlahoviček (mentor)
Committee memberKristian Vlahoviček (predsjednik povjerenstva)
Committee memberMirjana Kalafatić (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-02-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Amiotrofična lateralna skleroza (ALS) je progresivna neurodegenerativna bolest motoričkih neurona. Zaraženi motorički neuroni nakupljaju deformirane i netopljive proteine u proteinske inkluzije unutar aksona i staničnih tijela. Jedan od proteina prisutnih u tim inkluzijama je TDP-43. Između ostalog, taj protein ima ulogu u ekspresiji gena. Tijekom procesa poliadenilacije veže se na GU-bogat element 3’ UTR regije glasničke RNA b-aducina. U odsutstvu proteina TDP-43 primijećen je značajan pad razine ekspresije b-aducina. Cilj ovog istraživanja bio je upotrebom bioinformatičkih alata pronaći gene s motivima poliadenilacije slične onima u baducina, koji bi mogli vezati TDP-43. Podaci korišteni u ovome projektu (8 genoma kralješnjaka) su preuzeti s genomske baze podataka Ensembl. Napisao sam 17 programa koristeći Perl i Ensembl Perl API da bih izolirao otprilike 600 gena iz ljudskog genoma koji bi mogli biti regulirani s TDP-43. Devet od tih gena opisanih u prijašnjim radovima imali su različite razine ekspresije kod pacijenata s ALS-om. K tome, evolucijska očuvanost poliadenilacijskog signala i GU-bogatog elementa je uočena kod ostalih kralješnjaka.
Abstract (english)
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease of motor neurons. Affected motor neurons accumulate misshapen and insoluble proteins in proteinaceous inclusions within axons and cell bodies. One of the proteins present in those inclusions is TDP-43. That protein among other things has a role in gene expression. During the polyadenylation process it binds to the GU-rich element of the 3’ UTR region of b-adducin mRNA. In the absence of the protein there is a significant drop observed in the expression levels of b-adducin. The goal of this research was to find out if other genes have similar polyadenylation motifs as badducin, that could bind TDP-43, by using bioinformatics tools. The data used in the project (8 vertebrate genomes) was obtained from the Ensembl genome database. I wrote 17 scripts using the Perl programming language and Ensembl Perl API to isolate around 600 genes from the human genome that could possibly be regulated by TDP-43. Nine of those genes have been described in previous work done on the ALS to have different expression levels in patients with ALS. Additionally, evolutionary conservation of the polyadenylation signal and GU-rich element was observed in other vertebrate species.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:902958
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 09:18:28