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master's thesis
Epigenetička regulacija N-glikozilacije membranskih proteina HeLa stanica
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:716063
Barišić, Darko
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Barišić, D. (2012). Epigenetička regulacija N-glikozilacije membranskih proteina HeLa stanica (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:716063

Barišić, Darko. "Epigenetička regulacija N-glikozilacije membranskih proteina HeLa stanica." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:716063

Barišić, Darko. "Epigenetička regulacija N-glikozilacije membranskih proteina HeLa stanica." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:716063

Barišić, D. (2012). 'Epigenetička regulacija N-glikozilacije membranskih proteina HeLa stanica', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:716063

Barišić D. Epigenetička regulacija N-glikozilacije membranskih proteina HeLa stanica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:716063

D. Barišić, "Epigenetička regulacija N-glikozilacije membranskih proteina HeLa stanica", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:716063

Metadata
TitleEpigenetička regulacija N-glikozilacije membranskih proteina HeLa stanica
Title (english)Epigenetic regulation of surface N-glycosylation in HeLa cells
AuthorDarko Barišić
MentorVlatka Zoldoš (mentor)
Committee memberVlatka Zoldoš (predsjednik povjerenstva)
Committee memberInes Radanović (član povjerenstva)
Committee memberJasmina Rokov Plavec (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-10-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
N-glikozilacija proteina odnosi se na kovalentnu modifikaciju proteina važnu za njihovo pravilno smatanje i funkciju. Promjene u glikozilaciji membranskih proteina predstavljaju prvu frontu odgovora stanice na izmijenjeni okoliš, modificirajući signalne putove i samu fiziologiju stanice. Također, membranski glikoproteini čine ključan dio u prepoznavanju i reakciji na patogene i čimbenike imunološkog sustava. Epigenetički mehanizmi uključeni u ekspresiju gliko-gena (geni uključeni u N-glikozilaciju) predstavljaju jedan od načina regulacije sinteze glikana (nukleotida šećera) te time i cjelokupnog procesa glikozilacije proteina. Cilj ovog istraživanja je odrediti učinak modulacije epigenetičke informacije na ekspresiju N-vezanih površinskih glikana HeLa stanica, upotrebom epigenetičkih inhibitora DNA metiltransferaza i histonskih deacetilaza, te ispitati reverzibilnost izazvanih promjena N-glikoma. S obzirom da glikani membranskih proteina mogu odražavati tumorski i metastatski potencijal stanica, upotreba epigenetičkih inhibitora, čije djelovanje dovodi do promjena u ekspresiji površinskih glikana, bi mogla imati važnu ulogu u terapiji tumora.
Abstract (english)
N-glysoylation is a covalent modification of a protein important for its proper folding and function. Since cell surface represents the first front in cell-to-cell communication and communication between the cell and its environment, surface glycosylation can reflect changes in the environment as well as modify cellular signaling, recognition and adaptive changes in reaction to pathogens and factors of immune system. Changes in expression of glyco-genes through variety of epigenetic mechanisms represent one aspect of regulating glycan synthesis and protein glycosylation. To address the questions regarding the role of epigenetic information in N-glycoslylation, we studied changes in surface N-glycome of HeLa cells upon treatment with epigenetic inhibitors of DNA methyltransferases and histone deacetylasesas as well as reversibility of altered N-glycan profile. Since epigenetic inhibitors are currently explored as therapeutics in treatment of cancer, the presented work contributes to our understanding of their efficiency in altering the N-glycan profile of cancer cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:716063
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 09:24:55