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master's thesis
Utjecaj iona metala na aktivnost adenilosukcinat-sintetaze iz bakterije Helicobacter pylori; Priča o želatini i voću
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:552993
Srnec, Karla
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Srnec, K. (2021). Utjecaj iona metala na aktivnost adenilosukcinat-sintetaze iz bakterije Helicobacter pylori; Priča o želatini i voću (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:552993

Srnec, Karla. "Utjecaj iona metala na aktivnost adenilosukcinat-sintetaze iz bakterije Helicobacter pylori; Priča o želatini i voću." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:552993

Srnec, Karla. "Utjecaj iona metala na aktivnost adenilosukcinat-sintetaze iz bakterije Helicobacter pylori; Priča o želatini i voću." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:552993

Srnec, K. (2021). 'Utjecaj iona metala na aktivnost adenilosukcinat-sintetaze iz bakterije Helicobacter pylori; Priča o želatini i voću', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:552993

Srnec K. Utjecaj iona metala na aktivnost adenilosukcinat-sintetaze iz bakterije Helicobacter pylori; Priča o želatini i voću [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:552993

K. Srnec, "Utjecaj iona metala na aktivnost adenilosukcinat-sintetaze iz bakterije Helicobacter pylori; Priča o želatini i voću", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:552993

Metadata
TitleUtjecaj iona metala na aktivnost adenilosukcinat-sintetaze iz bakterije Helicobacter pylori; Priča o želatini i voću
Title (english)Effect of metal ions on activity of adenylosuccinate synthetase from bacterium Helicobacter pylori; The story of gelatin and fruit
AuthorKarla Srnec
MentorIvana Leščić Ašler (mentor)
MentorDraginja Mrvoš-Sermek (mentor)
Committee memberJasmina Rokov Plavec (predsjednik povjerenstva)
Committee memberDraginja Mrvoš-Sermek (član povjerenstva)
Committee memberIvana Biljan (član povjerenstva)
Committee memberTajana Begović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-07-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Ovaj diplomski rad sastoji se od dva dijela: istraživačkog i metodičkog. U istraživačkom dijelu rada pročišćene su tri varijante enzima adenilosukcinat-sintetaze (AdSS) iz bakterije Helicobacter pylori: AdSS obilježen histidinskim privjeskom, AdSS kojem je aspartat 12 zamijenjen alaninom i AdSS kojem je aspartat 12 zamijenjen asparaginom. Za pročišćavanje su korištene kromatografija ionske izmjene, afinitetna kromatografija i gel filtracija. Proteinima su potom mjerene specifične aktivnosti, a za obiljeţen AdSS određeni su kinetički parametri uz promjenjivu koncentraciju iona Mg2+ koristeći Michaelis-Menten model enzimske kinetike. Konačno, ispitan je utjecaj različitih iona metala na aktivnost obilježenog AdSS. U metodiĉkom dijelu rada pod naslovom „Priča o želatini i voću“ izrađena je metodičkodidaktička priprema za učenike četvrtog razreda gimnazije. Metodička priprema temeljena je na obrazovnim ishodima, najčešćim učeničkim krivim shvaćanjima i na uvodu u relevantnu literaturu. Pripremljeni materijal namijenjen je obrazovnoj strategiji učenja otkrivanjem.
Abstract (english)
This Diploma thesis consists of two parts: the research and methodological part. In the experimental part of diploma work, three variants of enzyme adenylosuccinate synthetase (AdSS) from bacterium Helicobacter pylori were purified: AdSS with histidine tag, AdSS with aspartate 12 changed to alanine and AdSS with aspartate 12 changed to asparagine. Ion exchange, affinity and gel filtration chromatographies were used for purification. Specific activity was measured for each enzyme variant, and for tagged AdSS kinetic parameters were also determined, by changing Mg2+ ions concentration, and using Michaelis-Menten model of enzyme kinetics. Finally, the effect of different metal ions on tagged AdSS activity was investigated. In the methodological part under the title “The story of gelatin and fruit” a didacticmethodological preparation was made for fourth grade high school students. The methodological preparation is based on the educational outcomes, common misconceptions held by students and with an insight in a revelant literature. The prepared material is intended for educational strategy of learning by discovery.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:552993
ProjectNumber: IP-2019-04-6764
Title: Alosterički komunikacijski putevi u oligomernim enzimima
Title: Allosteric communication pathways in oligomeric enzymes
Acronym: ALOKOMP/ALOCOMP
Leader: Zoran Štefanić
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Educational programme
Course: Educational programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije kemije (magistar/magistra edukacije kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-07-22
Terms of useLicence In copyright icon
Created on2021-09-10 13:56:06