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master's thesis
Pokazatelji toksičnosti nano čestica srebra u listovima duhana (Nicotiana tabacum L.)
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:537539
Beneta, Dijana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Beneta, D. (2012). Pokazatelji toksičnosti nano čestica srebra u listovima duhana (Nicotiana tabacum L.) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:537539

Beneta, Dijana. "Pokazatelji toksičnosti nano čestica srebra u listovima duhana (Nicotiana tabacum L.)." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:537539

Beneta, Dijana. "Pokazatelji toksičnosti nano čestica srebra u listovima duhana (Nicotiana tabacum L.)." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:537539

Beneta, D. (2012). 'Pokazatelji toksičnosti nano čestica srebra u listovima duhana (Nicotiana tabacum L.)', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:537539

Beneta D. Pokazatelji toksičnosti nano čestica srebra u listovima duhana (Nicotiana tabacum L.) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:537539

D. Beneta, "Pokazatelji toksičnosti nano čestica srebra u listovima duhana (Nicotiana tabacum L.)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:537539

Metadata
TitlePokazatelji toksičnosti nano čestica srebra u listovima duhana (Nicotiana tabacum L.)
Title (english)Toxicity of silver nanoparticles in tobacco (Nicotiana tabacum L.)
AuthorDijana Beneta
MentorSandra Radić Brkanac (mentor)
Committee memberSandra Radić Brkanac (predsjednik povjerenstva)
Committee memberIvančica Ternjej (član povjerenstva)
Committee memberVesna Benković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-02-09, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Nano čestice srebra se zbog svog antimikrobnog svojstva sve više koriste u proizvodima široke potrošnje (dezodoransima, parfemima, odjeći i obući, zavojima te u sredstvima za pranje i čišćenje) kao i u raznim industrijskim granama. Dosad je zabilježen fito- , cito- i genotoksični učinak nano čestica srebra na pojedine biljne i animalne organizme. U ovom je radu istražen toksični učinak nano čestica srebra (u rasponu koncentracija od 5 do 200 mg L-1) na listove duhana (Nicotiana tabacum L.) koji su uzorkovani nakon tjedan dana izlaganja. Određen je sadržaj klorofila i karotenoida te su izmjereni pojedini pokazatelji oksidacijskog stresa i aktivnosti antioksidacijskih enzima. Nano čestice srebra značajno su povećale sadržaj fotosintetskih pigmenata pri višim koncentracijama nano srebra. Zabilježen je i porast prolina te smanjenje sadržaja proteina. Nano čestice srebra su pri višim koncentracijama povećale oksidacijsko oštećenje lipida. Zamijećena je povećana aktivnost enzima superoksid dismutaze na tretmanima s najvišim koncentracijama nano srebra dok su porast sadržaja glutation reduktaze uzrokovale niže koncentracije nano srebra. Aktivnost askorbat peroksidaze, enzima koji razgrađuje vodikov peroksid, bila je povećana na svim tretmanima. Rezultati ovog istraživanja ukazuju da nano čestice srebra izazivaju oksidacijski stres u izdanku duhana, ali i na učinkovitost antioksidacijske obrane duhana pri koncentracijama tog toksičnog metala upotrebljenim u ovom radu.
Abstract (english)
Nanoparticles of silver, due to its antimicrobial properties, are increasingly used in consumer products (deodorants, perfumes, clothes and shoes, bandages and in cleaning solutions and sprays) as well as in various industries. So far, phyto-, cyto-and genotoxic effects of silver nanoparticles to the individual plant and animal organisms were observed. This paper investigated the toxic effect of silver nano particles (in the concentration range of 5-200 mg L-1) in tobacco (Nicotiana tabacum L.). Plants were harvested and analyzed after 7 days. Chlorophylls and carotenoid contents, certain indicators of oxidative stress and antioxidant enzyme activities were measured. Silver nanoparticles, at higher concentrations, significantly increased the contents of photosynthetic pigments. Also, an increase of proline and decrease in protein content was noticed. At higher concentrations, silver nanoparticles increased oxidative damage to membrane lipids. An increased activity of the enzyme superoxide dismutase has been noticed under higher nano silver treatments, while the induction of glutathione reductase was caused by lower nano silver concentrations. The activity of ascorbate peroxidase was increased at all treatments. The results of the study indicate that silver nano particles cause oxidative stress in N. tabacum leaves, but they also show the efficiency of antioxidative defense system of the tobacco within the range of nano silver concentrations used in the work.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:537539
Study programmeTitle: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 09:37:23