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master's thesis
Uloga 2D i 3D staničnog okruženja u odgovoru tumorskih stanica na stres
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:715630
Petelinec, Ana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Petelinec, A. (2021). Uloga 2D i 3D staničnog okruženja u odgovoru tumorskih stanica na stres (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:715630

Petelinec, Ana. "Uloga 2D i 3D staničnog okruženja u odgovoru tumorskih stanica na stres." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:715630

Petelinec, Ana. "Uloga 2D i 3D staničnog okruženja u odgovoru tumorskih stanica na stres." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:715630

Petelinec, A. (2021). 'Uloga 2D i 3D staničnog okruženja u odgovoru tumorskih stanica na stres', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:715630

Petelinec A. Uloga 2D i 3D staničnog okruženja u odgovoru tumorskih stanica na stres [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:715630

A. Petelinec, "Uloga 2D i 3D staničnog okruženja u odgovoru tumorskih stanica na stres", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:715630

Metadata
TitleUloga 2D i 3D staničnog okruženja u odgovoru tumorskih stanica na stres
Title (english)The role of 2D and 3D cell environment in the tumor cellular stress response
AuthorAna Petelinec
MentorJuraj Simunić (mentor)
MentorIvana Ivančić Baće (sumentor)
Committee memberIvana Ivančić Baće (predsjednik povjerenstva)
Committee memberInes Radanović (član povjerenstva)
Committee memberDraginja Mrvoš-Sermek (član povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-09-10, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Stanično okruženje i međustanična komunikacija su jedan od bitnih čimbenika u odgovoru stanica na stres. U 3D kulturama stanice su u okruženju koje je najsličnije uvjetima u tkivima, dok u 2D kulturama stanice rastu na podlozi u jednom sloju i ne stvara se izvanstanični matriks. Jedan od tipova međustanične komunikacije su premošćujuće strukture koje nastaju između stanica. To su tunelirajuće nanocjevčice (engl. tunneling nanotubes, TNT) i tumorske mikrocjevčice (engl. tumor microtubes, TM). U ovom radu opisane su premošćujuće strukture u stanicama U2OS. Utvrđeno je da tretman hladnoćom povećava broj premošćujućih struktura u uzorcima dok tretman inhibitorom proteina sHSP27 J2 utječe na smanjenje broja premošćujućih struktura. Također, zabilježena je lokalizacija proteina sHSP27 i membranskih struktura koja upućuje na njihov transport. Uspostavljen je protokol za izradu 3D kultura metodom magnetske levitacije stanica. Uspoređen je izgled stanica u 2D i 3D staničnom okruženju i razina oksidativnog stresa mjerena bojom za detekciju stresa CellROXTM Deep Red Reagent.
Abstract (english)
The cell environment and intercellular connections have major impact in cellular stress response. Cell environment in 3D cultures is the most similar to the one in tisssues, while at 2D cultures cells grow on substrate in a single layer and don’t produce extracellular matrix. One type of cell to cell connections are structures that interconnect the cells. These are tunneling nanotubes (TNTs) and tumor microtubes, (TMs). In this paper, tunneling structures in U2OS cells are described. Cold shock treatment was found to increase the number of TNTs and TMs, while the treatment with sHSP27 inhibitor J2 reduced the number of structures. Also, localization of protein sHSP27 and membrane structures that refer to the transport is noted. A protocol for making 3D cultures by magnetic levitation has been established. The appearance of cells in a 2D and 3D cell environment and the level of oxidative stress measured by CellROXTM Deep Red Reagent stress detection were compared.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:715630
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-09-20 13:08:50