prikaz prve stranice dokumenta Interakcije proteina p53 te karakterizacija stanica otpornih na ciljanu terapiju vemurafenibom u melanomu čovjeka
  Download
PDF 6.07 MB
doctoral thesis
Interakcije proteina p53 te karakterizacija stanica otpornih na ciljanu terapiju vemurafenibom u melanomu čovjeka
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:320600
Radić, Martina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Radić, M. (2021). Interakcije proteina p53 te karakterizacija stanica otpornih na ciljanu terapiju vemurafenibom u melanomu čovjeka (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:320600

Radić, Martina. "Interakcije proteina p53 te karakterizacija stanica otpornih na ciljanu terapiju vemurafenibom u melanomu čovjeka." Doctoral thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:320600

Radić, Martina. "Interakcije proteina p53 te karakterizacija stanica otpornih na ciljanu terapiju vemurafenibom u melanomu čovjeka." Doctoral thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:320600

Radić, M. (2021). 'Interakcije proteina p53 te karakterizacija stanica otpornih na ciljanu terapiju vemurafenibom u melanomu čovjeka', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 01 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:320600

Radić M. Interakcije proteina p53 te karakterizacija stanica otpornih na ciljanu terapiju vemurafenibom u melanomu čovjeka [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 December 01] Available at: https://urn.nsk.hr/urn:nbn:hr:217:320600

M. Radić, "Interakcije proteina p53 te karakterizacija stanica otpornih na ciljanu terapiju vemurafenibom u melanomu čovjeka", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:320600

Metadata
TitleInterakcije proteina p53 te karakterizacija stanica otpornih na ciljanu terapiju vemurafenibom u melanomu čovjeka
Title (english)Protein interactions of p53 and characterization of cells resistant to targeted therapy by vemurafenib in human melanoma
AuthorMartina Radić
MentorMaja Herak-Bosnar (mentor)
Committee memberNeda Slade (predsjednik povjerenstva)
Committee memberIgor Weber (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-03-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Melanom je jedan od najagresivnijih tumora čovjeka te je vodeći uzrok smrti oboljelih od tumora kože. Iako su saznanja o molekularnoj pozadini bolesti dovela su do otkrića novih terapija, dugotrajno liječenje onemogućeno je pojavom otpornosti. Iznenađujuće, iako je u melanomu tumorski supresor p53 rijetko mutiran, ne obavlja svoju funkciju u suzbijanju tumora. Glavni fokus našeg rada otkrivanje je mehanizama otpornosti na ciljanu terapiju vemurafenibom te istraživanje proteinskih interakcija proteina p53 koje bi mogle biti jedan od razloga njegove inaktivacije. Razvili smo i okarakterizirali stanice melanoma otporne na vemurafenib te proučili interakcije proteina p53 s članovima proteinskih porodica p53, NME i GLI. Naši rezultati pokazuju aktivaciju signalnog puta MAPK i PI3K/AKT pojavom otpornosti na terapiju. Također, pokazali smo da protein p53 ostvaruje interakcije s pojedinim izoformama proteina p53 i p73, ali ne i s članovima porodice NME i GLI. Ovim radom smo ukazali na moguću važnost interakcija unutar porodice p53.
Abstract (english)
Melanoma is one of the most aggressive human tumors and the leading cause of death of skin cancer patients. Even though the molecular understanding of underlying mechanisms driving melanoma has led to the discovery of new therapies, long-term success has been hampered by the development of drug resistance. Surprisingly, although the tumor suppressor p53 is rarely mutated in melanoma, its tumor-suppressive function seems to be impaired. The main focus of this work is the discovery of mechanisms of resistance to targeted therapy by vemurafenib together with the study of protein interactions of p53 that could be one of the causes of its inactivation. We developed and characterized vemurafenib-resistant melanoma cells and studied the interactions of p53 with members of the p53, NME, and GLI protein families. Our results show activation of the MAPK and PI3K/AKT signaling pathways after the development of resistance. We further show that the p53 protein interacts with individual isoforms of the p53 and p73 proteins, but not with members of the NME and GLI families. In this work, we pointed out the possible importance of interactions within the p53 family.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:320600
Promotion2021
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-03-29
Terms of useLicence In copyright icon
Created on2021-09-21 10:43:07