prikaz prve stranice dokumenta Utjecaj lizata trombocita na biološke značajke mezenhimskih matičnih stanica uzgojenih in vitro
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master's thesis
Utjecaj lizata trombocita na biološke značajke mezenhimskih matičnih stanica uzgojenih in vitro
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:220296
Božinović, Ksenija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Božinović, K. (2012). Utjecaj lizata trombocita na biološke značajke mezenhimskih matičnih stanica uzgojenih in vitro (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:220296

Božinović, Ksenija. "Utjecaj lizata trombocita na biološke značajke mezenhimskih matičnih stanica uzgojenih in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:220296

Božinović, Ksenija. "Utjecaj lizata trombocita na biološke značajke mezenhimskih matičnih stanica uzgojenih in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:220296

Božinović, K. (2012). 'Utjecaj lizata trombocita na biološke značajke mezenhimskih matičnih stanica uzgojenih in vitro', Master's thesis, University of Zagreb, Faculty of Science, accessed 13 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:220296

Božinović K. Utjecaj lizata trombocita na biološke značajke mezenhimskih matičnih stanica uzgojenih in vitro [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 May 13] Available at: https://urn.nsk.hr/urn:nbn:hr:217:220296

K. Božinović, "Utjecaj lizata trombocita na biološke značajke mezenhimskih matičnih stanica uzgojenih in vitro", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:220296

Metadata
TitleUtjecaj lizata trombocita na biološke značajke mezenhimskih matičnih stanica uzgojenih in vitro
Title (english)BIOLOGICAL PROPERTIES OF MESENCHYMAL STEM CELLS EXPANDED IN VITRO IN MEDIA WITH DIFFERENT HUMAN PLATELET LYSATE CONTENT
AuthorKsenija Božinović
MentorMirna Golemović (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberMirna Golemović (član povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberVesna Benković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-07-03, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
U ovom istraživanju uzgajane su mezenhimske matične stanice (MMS) izolirane iz koštane srži (KS) tri dobrovoljna davatelja. MMS ispoljavaju imunomodulatorna svojstva in vitro što im daje potencijalnu primjenu pri transplantaciji krvotvornih matičnih stanica i liječenju reakcije presatka protiv primatelja. Učestalost MMS u KS je vrlo mala te ih je za potrebe kliničke primjene neophodno umnožiti in vitro. Za umnažanje MMS korištene su kultivacijske podloge oplemenjene s različitim udjelima lizata ljudskih trombocita (LT): 10, 8, 6, 4 ili 2% LT. MMS su uzgajane tijekom pet ciklusa presađivanja. Na kraju svakog ciklusa je analizirana kinetika rasta stanica, morfologija te imunofenotip uzgajanih MMS in vitro. Nakon drugog ciklusa umnažanja ispitan je adipogeni potencijal MMS. Tijekom cijelog perioda uzgoja in vitro, u svim uvjetima uzgoja stanice su ispoljavale mezenhimske karakteristike; prihvaćale su se na plastičnu podlogu, poprimile vretenasti fibroblastni izgled, ispoljavale imunofenotip MMS i uspješno diferencirale u zrele adipocite. Utvrđeno je da različiti udjeli LT ne utječu na navedene biološke značajke MMS, ali da imaju značajan utjecaj na kinetiku rasta MMS. Stanična populacija uzgojena u uvjetu s većim udjelom LT u kultivacijskoj podlozi se više umnažala u odnosu na stanice uzgajane u prisustvu manjeg udjela LT. Vrijeme udvostručenja stanica bilo je obrnuto proporcionalno udjelu LT: što je bio veći udio, stanice su se brže udvostručile.
Abstract (english)
Mesenchymal stem cells (MSCs) show immunomodulatory properties in vitro that make them interesting in the field of cellular therapy. MSCs have been clinically applied to improve engraftment in hematopoietic stem cell transplantation and to treat acute graft versus host disease. Incidence of MSCs in bone marrow (BM) is very low and in vitro expansion of MSCs is necessary. In this study MSCs derived from BM of three healthy donors were expanded in vitro. Cultivation mediums enriched with different proportion of human platelet lysate (PL) were used to expand MSCs: 10, 8, 6, 4 or 2% PL. MSCs were expanded through five passages. Cell growth kinetics, morphology and immunophenotype of expanded MSCs were analyzed. Following second passage, a small proportion of cells were used for analysis of adipogenic potential of expanded cells. During the whole period of cultivation in vitro, MSCs continuously expressed mesenchymal characteristics in all culture coditions: they adhered to plastic surface showing fibroblast shape, they expressed mesenchymal immunophenotype and differentiated into adypocites. Different content of PL in culture medium had no effect on investigated biological characteristics of the MSCs, but did have significant impact on the growth kinetics of MSC. With higher content of PL, number of harvested cells increased. Cell doubling time was inversely proportional to the content of PL in the medium.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:220296
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 12:16:17