prikaz prve stranice dokumenta Odnos osteogenog potencijala i matičnosti tijekom diferencijacije mezenhimskih matičnih stanica
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master's thesis
Odnos osteogenog potencijala i matičnosti tijekom diferencijacije mezenhimskih matičnih stanica
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:551751
Brkić, Šime
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Brkić, Š. (2012). Odnos osteogenog potencijala i matičnosti tijekom diferencijacije mezenhimskih matičnih stanica (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:551751

Brkić, Šime. "Odnos osteogenog potencijala i matičnosti tijekom diferencijacije mezenhimskih matičnih stanica." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:551751

Brkić, Šime. "Odnos osteogenog potencijala i matičnosti tijekom diferencijacije mezenhimskih matičnih stanica." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:551751

Brkić, Š. (2012). 'Odnos osteogenog potencijala i matičnosti tijekom diferencijacije mezenhimskih matičnih stanica', Master's thesis, University of Zagreb, Faculty of Science, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:551751

Brkić Š. Odnos osteogenog potencijala i matičnosti tijekom diferencijacije mezenhimskih matičnih stanica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:217:551751

Š. Brkić, "Odnos osteogenog potencijala i matičnosti tijekom diferencijacije mezenhimskih matičnih stanica", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:551751

Metadata
TitleOdnos osteogenog potencijala i matičnosti tijekom diferencijacije mezenhimskih matičnih stanica
Title (english)Relation of osteogenic potential and stemness during mesenchymal stem cell differentiation
AuthorŠime Brkić
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberGordana Lacković-Venturin (član povjerenstva)
Committee memberIvana Ivančić Baće (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-12-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Mezenhimske matične stanice (hMSC) predstavljaju malu populaciju somatskih matičnih stanica koja je prisutna u koštanoj srži i većini vezivnih tkiva. hMSC su multipotentne te ih sposobnost diferenciranja u osteoblaste čini pogodnima za primjenu u regenerativnoj ortopediji. U ovom radu proučavan je stupanj osteogene diferencijacije hMSC nakon tretiranja osteoinduktivnim molekulama po standardnom protokolu. In vitro diferencijacija je praćena analizom koštanih markera – alkalne fosfataze i koštanog sijaloproteina, te analizom markera matičnosti. Oct4, Sox2 i Nanog su markeri pluripotentnosti embrionalnih matičnih stanica, a izraženi su i u nekim tipovima somatskih matičnih stanica. Cilj ovog rada je ispitati da li su oni izraženi u hMSC te opada li njihova izraženost tijekom diferencijacije. Rezultati RT qPCRa su pokazali da su Oct4, Sox2 i Nanog izraženi u nediferenciranim hMSC te im sa pojavom i porastom koštanih markera opada izraženost. Imunocitokemijska analiza pokazala je da su hMSC negativne na SOX2 i NANOG, a pozitivne na OCT4 protein. Razina ekspresije OCT4 je ostala nepromijenjena tijekom diferencijacije.
Abstract (english)
Mesenchymal stem cells (hMSC) represent a small population of cells located in bone marrow and most of the connective tissue. hMSC are multipotent and their ability to differentiate into osteoblast makes them suitable for application in regenerative orthopedics. In this research, I investigated the level of osteogenic differentiation of hMSC after the treatmant with osteoinductive molecules under the standard protocol. In vitro differentiation was evaluated by the analysis of bone markers – alkaline phosphatase and bonesialoprotein and by analysis of markers of stemness. Oct4, Sox2 i Nanog are pluripotency markers of embryonic stem cells but their expression was also confirmed in some types of adult stem cells. The aim of this research was to investigate if they were expressed in hMSC and if their expression decreases during differentiation. Results of RT qPCR showed that Oct4, Sox2 i Nanog are expressed in undifferentiated hMSC and that their expression decreases paralel with the apperance and increase of bone markers. Immuncitochemistry analysis showed that hMSC are negative for SOX2 and NANOG proteins, but they are positive for OCT4. The level of expression of OCT4 did not change during differentiation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:551751
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 12:19:41