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master's thesis
Istraživanje vezanja bisbenzimida Hoechst 33258 s DNA/RNA spektroskopijom površinski pojačanog Ramanovog raspršenja
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:970257
Zavidić, Virna
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Zavidić, V. (2021). Istraživanje vezanja bisbenzimida Hoechst 33258 s DNA/RNA spektroskopijom površinski pojačanog Ramanovog raspršenja (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:970257

Zavidić, Virna. "Istraživanje vezanja bisbenzimida Hoechst 33258 s DNA/RNA spektroskopijom površinski pojačanog Ramanovog raspršenja." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:970257

Zavidić, Virna. "Istraživanje vezanja bisbenzimida Hoechst 33258 s DNA/RNA spektroskopijom površinski pojačanog Ramanovog raspršenja." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:970257

Zavidić, V. (2021). 'Istraživanje vezanja bisbenzimida Hoechst 33258 s DNA/RNA spektroskopijom površinski pojačanog Ramanovog raspršenja', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:970257

Zavidić V. Istraživanje vezanja bisbenzimida Hoechst 33258 s DNA/RNA spektroskopijom površinski pojačanog Ramanovog raspršenja [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:970257

V. Zavidić, "Istraživanje vezanja bisbenzimida Hoechst 33258 s DNA/RNA spektroskopijom površinski pojačanog Ramanovog raspršenja", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:970257

Metadata
TitleIstraživanje vezanja bisbenzimida Hoechst 33258 s DNA/RNA spektroskopijom površinski pojačanog Ramanovog raspršenja
Title (english)Binding study of bisbenzimide Hoechst 33258 with DNA/RNA using surface-enhanced Raman scattering spectroscopy
AuthorVirna Zavidić
MentorSnežana Miljanić (mentor)
Committee memberSnežana Miljanić (predsjednik povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberAdriana Kenđel (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Spektroskopijom površinski pojačanog Ramanovog raspršenja (SERS) istražene su interakcije molekula Hoechst 33258 (H33258) s dvolančanim sintetskim DNA/RNA polinukleotidima (poli dA-poli dT, poli dG-poli dC, poli rA-poli rU) te prirodnom ct-DNA. Kompleksi su pripravljeni u molarnim omjerima [H33258]/[polinukleotid] = 1/1, 1/5 i 1/10 u koloidnim suspenzijama srebrovih nanočestica stabiliziranih površinskim slojem negativno nabijenih kloridnih iona (AgNP_Cl) i pozitivno nabijenih molekula spermina (AgNP_sp). Prije analize načina vezanja s polinukleotidima, istražena je agregacija H33258 u rasponu koncentracija 1×106–1×104 mol/L, pri čemu su opaženi SERS spektri karakteristični za agregirane i monomerne vrste. SERS spektri H33258 s DNA analozima ukazali su na vezanje molekula u mali utor AT polinukleotida te djelomično interkaliranje između parova baza gvanina i citozina pristupom kroz veliki utor GC polinukleotida. Promjene u SERS spektrima kompleksa s RNA analogom, upućuju na interakcije male molekule s adeninom. Spektralne karakteristike H33258 dominiraju u spektrima kompleksa s ct-DNA, najvjerojatnije zbog nasumičnih sljedova AT i GC prirodnog polinukleotida.
Abstract (english)
Interactions of Hoechst 33258 (H33258) molecules with double-stranded synthetic DNA/RNA polynucleotides (poly dA-poly dT, poly dG-poly dC, poly rA-poly rU) and natural ct-DNA were studied by surface-enhanced Raman scattering (SERS) spectroscopy. Complexes were prepared in molar ratios [H33258]/[polynucleotide] = 1/1, 1/5 and 1/10 in colloidal suspensions of silver nanoparticles stabilized by a surface layer of negatively charged chloride ions (AgNP_Cl) and positively charged spermine molecules (AgNP_sp). Prior to the polynucleotide binding assay, aggregation of H33258 in the concentration range 1×106–1×104 mol/L was investigated, whereby SERS spectra characteristic of aggregated and monomeric species were observed. The SERS spectra of H33258 with DNA analogs indicated binding of molecules into the minor groove of AT polynucleotide and partial intercalation between guanine and cytosine base pairs by access through the major groove of GC polynucleotide. Changes in the SERS spectra of the complex with the RNA analog implied interactions of small molecules with adenine. The spectral characteristics of H33258 dominated in the spectra of complexes with ct-DNA, most likely due to random AT and GC sequences of the natural polynucleotide.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:970257
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-09-24
Terms of useLicence In copyright icon
Created on2021-09-30 08:34:29