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master's thesis
Ispitivanje interakcija imidazo-piridinskih derivata s DNA i RNA spektroskopskim metodama
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:567642
Grgić, Lea
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Grgić, L. (2021). Ispitivanje interakcija imidazo-piridinskih derivata s DNA i RNA spektroskopskim metodama (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:567642

Grgić, Lea. "Ispitivanje interakcija imidazo-piridinskih derivata s DNA i RNA spektroskopskim metodama." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:567642

Grgić, Lea. "Ispitivanje interakcija imidazo-piridinskih derivata s DNA i RNA spektroskopskim metodama." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:567642

Grgić, L. (2021). 'Ispitivanje interakcija imidazo-piridinskih derivata s DNA i RNA spektroskopskim metodama', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:567642

Grgić L. Ispitivanje interakcija imidazo-piridinskih derivata s DNA i RNA spektroskopskim metodama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:567642

L. Grgić, "Ispitivanje interakcija imidazo-piridinskih derivata s DNA i RNA spektroskopskim metodama", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:567642

Metadata
TitleIspitivanje interakcija imidazo-piridinskih derivata s DNA i RNA spektroskopskim metodama
Title (english)Study of the interaction of imidazo-pyridine derivatives with DNA and RNA by spectroscopic methods
AuthorLea Grgić
MentorMarijana Radić Stojković (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
Committee memberDubravka Matković-Čalogović (član povjerenstva)
Committee memberNives Galić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U ovom diplomskom radu spektroskopski su okarakterizirane vodene otopine šest derivata imidazo[4,5-b]piridina s molekulama DNA i RNA. Spojevi apsorbiraju u području 230 ̶ 500 nm i utvrđeno je da u tom koncentracijskom području, ne agregiraju. Ispitane su interakcije spojeva s različitim dvolančanim polinukleotidima koristeći UV/Vis-spektroskopiju (termička denaturacija polinukleotida), fluorimetriju i spektroskopiju cirkularnog dikroizma (CD). Zamijećeno je da na promjenu fluorescencije u titracijama s DNA i RNA utječe položaj amino bočnih ogranaka na tetracikličkom prstenu. Na jačinu interakcije s DNA/RNA utjecali su i položaj amino bočnog ogranka i naboj liganda. Ispitivani spojevi su akiralni te nemaju CD spektar. Mjerenja CD sa spojevima s amino bočnim ogrankom na C2 položaju upućuju na mješoviti način vezanja tih derivata u DNA i RNA. Promjene u CD spektrima s piperazinskim derivatima upućuju na interkalativni način vezanja, dok kod spojeva s amino bočnim ogrankom na položaju C5 upućuju na nespecifično vezanje agregiranih spojeva uz okosnicu DNA/RNA.
Abstract (english)
Water solutions of six imidazo[4,5-b]pyridine derivatives with DNA and RNA were spectroscopically characterized. The compounds absorb in the 230-500 nm range and do not aggregate in that concentration range. Interactions of compounds with different double-stranded polynucleotides were studied using UV/Vis spectroscopy (thermal denaturation of polynucleotides), fluorimetry and CD spectroscopy. The change in fluorescence in the titrations with DNA and RNA was influenced by the position of the amino side pendants on the tetracyclic ring. DNA/RNA interaction strength was also influenced by the position of the amino side pendant and lingand's charge. These compunds are achiral and do not have CD spectrum. CD measurements of derivatives with an amino side pendant at the C2 position suggest a mixed binding mode to DNA and RNA. Changes in the CD spectra with piperazine derivatives indicate intercalation binding, whereas compounds with an amino side pendant at the C5 position indicate nonspecific binding of aggregated compounds along the DNA/RNA backbone.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:567642
ProjectNumber: IP-2018-01-4694
Title: Molekularno prepoznavanje DNA:RNA hibridnih i višelančanih struktura u bioanalitičkim i in vitro sustavima
Acronym: DNARNAHyB-MolBio
Leader: Marijana Radić Stojković
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-09-23
Terms of useLicence In copyright icon
Created on2021-09-30 09:11:04