prikaz prve stranice dokumenta Effect of Tff3 protein deficiency on expression of neurodegeneration-associated genes in hippocampi of aged mice
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master's thesis
Effect of Tff3 protein deficiency on expression of neurodegeneration-associated genes in hippocampi of aged mice
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:200779
Anušić, Lucija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Anušić, L. (2021). Effect of Tff3 protein deficiency on expression of neurodegeneration-associated genes in hippocampi of aged mice (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:200779

Anušić, Lucija. "Effect of Tff3 protein deficiency on expression of neurodegeneration-associated genes in hippocampi of aged mice." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:200779

Anušić, Lucija. "Effect of Tff3 protein deficiency on expression of neurodegeneration-associated genes in hippocampi of aged mice." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:200779

Anušić, L. (2021). 'Effect of Tff3 protein deficiency on expression of neurodegeneration-associated genes in hippocampi of aged mice', Master's thesis, University of Zagreb, Faculty of Science, accessed 17 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:200779

Anušić L. Effect of Tff3 protein deficiency on expression of neurodegeneration-associated genes in hippocampi of aged mice [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 May 17] Available at: https://urn.nsk.hr/urn:nbn:hr:217:200779

L. Anušić, "Effect of Tff3 protein deficiency on expression of neurodegeneration-associated genes in hippocampi of aged mice", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:200779

Metadata
TitleEffect of Tff3 protein deficiency on expression of neurodegeneration-associated genes in hippocampi of aged mice
Title (croatian)Učinak nedostatka proteina Tff3 na ekspresiju gena povezanih s neurodegeneracijom hipokampusa u mišjem modelu starenja
AuthorLucija Anušić
MentorMirela Baus Lončar (mentor)
MentorSofia Ana Blažević (sumentor)
Committee memberSofia Ana Blažević (predsjednik povjerenstva)
Committee memberInga Urlić (član povjerenstva)
Committee memberMarin Ježić (član povjerenstva)
Committee memberPetra Peharec Štefanić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
The increase in the incidence of neurodegenerative diseases is a reflection of complex cumulative processes during aging which involve the interactions of numerous organ systems. Decreased levels of Trefoil factor 3 (Tff3) protein in the cerebrospinal fluid of patients with Alzheimer’s disease indicate its possible role in neurodegenerative processes. Tff3 is involved in a variety of processes such as healing of mucosal surfaces in the gastrointestinal tract and apoptosis, migration, and angiogenesis, while its role in neural tissue is still unknown. In this paper, we hypothesized that Tff3 protein deficiency leads to a greater affinity of mice for the development of neurodegenerative processes, which we measured through the relative expression of the following genes:brain-derived neurotrophic factor (Bdnf), exchange protein directly activated by cAMP 2 (Epac2), epidermal growth factor receptor (Egfr), neurofilament light protein (Nfl) and L1 cell adhesion molecule (L1cam). Two - year - old mice of the Tff3 -/- C57Bl6/NCrl genotype and appropriate Wt controls were used in this study. Our results showed that Tff3 protein deficiency does not affect the expression of the observed genes involved in neurodegenerative processes.
Abstract (croatian)
Porast incidencije neurodegerativnih bolesti odraz je kompleksnih kumulativnih procesa tijekom starenja te one uključuju interakcije brojnih organskih sustava. Smanjena razina Trefoil factor 3 (Tff3) proteina u likvoru Alzheimer pacijenata ukazuje na njegovu moguću ulogu u neurodegenerativnim procesima. Tff3 u gastrointestinalnoj mukozi sudjeluje u apoptozi, migraciji i angiogenezi, dok je njegova uloga u neuralnom tkivu gdje je prisutan još nepoznata. U ovom radu, postavili smo hipotezu da nedostatak proteina Tff3 dovodi do veće sklonosti miševa prema razvoju neurodegenerativnih procesa, koje smo mjerili putem relativne ekspresije gena Bdnf, Epac2, Nfl, Egfr i L1cam. U ovom radu korišteni su miševi stari dvije godine genotipa Tff3-/- C57Bl6/NCrl te odgovarajuće Wt kontrole. Naši rezultati pokazali su da nedostatak Tff3 proteina ne utječe na ekspresiju promatranih gena uključenih u neurodegenerativne procese.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:200779
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-09-24
Terms of useLicence In copyright icon
Created on2021-10-05 12:47:54