Adenosine-phosphates are organic molecules from the group of nucleotides, which include adenosine-5'-triphosphate (ATP), adenosine-5'-diphosphate (ADP) and adenosine-5'-monophosphate (AMP). Quantitative determination of adenosine-phosphates has a pronounced diagnostic role because the adenosine-phosphate concentration has influence on many physiological conditions and is directly correlated with the homeostasis of the organism, e.g. energy charge. For these reasons, the aim of this study was to develop a widely applicable, accurate and precise method of adenosine-phosphate extraction from a small volume of capillary blood. To achieve this goal, in addition to the original scientific contribution, it was necessary to examine the binding of adenosine-phosphates to activated carbon by adsorption chromatography for the purpose of blood samples purification by solid phase micro-extraction. Activated carbon is often used as an affinity adsorbent in a range of pharmaceutical, medical and biological applications due to its wide availability, large active surface area relative to mass and unique biological and physicochemical properties. It was necessary to develop and validate micro-extraction method by reverse-phase liquid chromatography, which separates, detects and quantifies adenosine-phosphates extracted from a capillary blood sample. The analyzed concentrations of adenosine-phosphates from the blood correspond to the theoretical values found in literature, which indicates the reliability of developed extraction method. Thus, activated carbon selectively binds adenosine-phosphates and purifies the blood sample, while the chromatographic parameters linearly, selectively and precisely separate these analytes.