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master's thesis
Utjecaj nedostatka Tff3 proteina na ekspresiju jetrenih gena uključenih u apoptozu i metabolizam lipida i glukoze u mišjem modelu starenja
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:525018
Štambuk, Jelena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Štambuk, J. (2021). Utjecaj nedostatka Tff3 proteina na ekspresiju jetrenih gena uključenih u apoptozu i metabolizam lipida i glukoze u mišjem modelu starenja (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:525018

Štambuk, Jelena. "Utjecaj nedostatka Tff3 proteina na ekspresiju jetrenih gena uključenih u apoptozu i metabolizam lipida i glukoze u mišjem modelu starenja." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:525018

Štambuk, Jelena. "Utjecaj nedostatka Tff3 proteina na ekspresiju jetrenih gena uključenih u apoptozu i metabolizam lipida i glukoze u mišjem modelu starenja." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:525018

Štambuk, J. (2021). 'Utjecaj nedostatka Tff3 proteina na ekspresiju jetrenih gena uključenih u apoptozu i metabolizam lipida i glukoze u mišjem modelu starenja', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:525018

Štambuk J. Utjecaj nedostatka Tff3 proteina na ekspresiju jetrenih gena uključenih u apoptozu i metabolizam lipida i glukoze u mišjem modelu starenja [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:525018

J. Štambuk, "Utjecaj nedostatka Tff3 proteina na ekspresiju jetrenih gena uključenih u apoptozu i metabolizam lipida i glukoze u mišjem modelu starenja", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:525018

Metadata
TitleUtjecaj nedostatka Tff3 proteina na ekspresiju jetrenih gena uključenih u apoptozu i metabolizam lipida i glukoze u mišjem modelu starenja
Title (english)Effect of Tff3 protein deficiency on expression of apoptotic, lipid and glucose metabolisam relevant genes in aging mouse model
AuthorJelena Štambuk
MentorMirela Baus Lončar (mentor)
MentorSofia Ana Blažević (sumentor)
Committee memberSofia Ana Blažević (predsjednik povjerenstva)
Committee memberBožena Mitić (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberSven Jelaska (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Tff3 protein pripada skupini Tff proteina koje karakterizira domena s tri petlje nalik trolisnoj djetelini po kojoj su dobili ime. Eksprimiran je u raznim tkivima te ima višestruku funkciju, ovisno o mjestu ekspresije i uvjetima u stanici. Dosadašnja istraživanja su pokazala da Tff3 ima antiapoptotsko djelovanje te da utječe na metabolizam glukoze i lipida u jetri, no točni mehanizmi njegova djelovanja nisu još do kraja proučeni. Apoptoza i metabolizam jetre se mijenjaju starenjem zbog nakupljanja promjena u organizmu. Starenjem se u jetri povećava učestalost apoptoze, dolazi do nakupljanja lipida i smanjene β-oksidacije i ketogeneze što često rezultira razvojem bolesti. Cilj ovog istraživanja bio je proučiti utjecaj nedostatka Tff3 na ekspresiju apoptotskih gena Bax, Smac, Cyc te gena vezanih uz metabolizam jetre Hmgcs2, Cpt1a, Gk u jetri miševa starih dvije godine. Ekspresiju gena pratili smo metodom kvantitativne lančane reakcije polimerazom, koju smo prethodno optimizirali podešavajući uvjete reakcije i isprobavanjem različitih početnica za relevantne gene. Usporedbom ekspresije gena miševa s nedostatkom proteina sa divljim tipom i statističkom analizom ustanovili smo da nije došlo do statistički značajne promjene, odnosno da nedostatak Tff3 proteina nije izazvao promjenu u ekspresiji ovih jetrenih gena u starim miševima.
Abstract (english)
The Tff3 protein belongs to a group of trefoil factor family (Tff) peptides that are characterized by the three-loop clover-like domain. It is expressed in multiple tissues and has various functions including effects on apoptosis. The exact function depends on the site of expression and conditions in the cell. Tff3 affects glucose and lipid metabolism in the liver. The exact mechanisms of its action are still a mystery. Apoptosis and liver metabolism change during aging due to progressive changes in the organism. Pathological changes during aging are caused by increased frequency of apoptosis and lipid accumulation, decreases β-oxidation and ketogenesis. This research aimed to study the effect of Tff3 deficiency on the expression of apoptotic genes Bax, Smac, Cyc, and genes related to liver metabolism Hmgcs2, Cpt1a, Gk in the liver of two-year-old mice. We used the quantitative polymerase chain reaction method, but first, we optimized the method by adjusting the conditions and testing different primers for relevant genes. Comparison of gene expression of protein-deficient mice with wild-type and statistical analysis revealed no statistically significant changes. Tff3 protein deficiency did not cause a change in the expression of these liver genes in old mice.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:525018
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-09-24
Terms of useLicence In copyright icon
Created on2021-10-08 10:35:22