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master's thesis
Učinak glukoze na fitotoksičnost nanočestica i iona srebra u algi Chlorella vulgaris
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:486947
Đaković, Matea
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Đaković, M. (2021). Učinak glukoze na fitotoksičnost nanočestica i iona srebra u algi Chlorella vulgaris (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:486947

Đaković, Matea. "Učinak glukoze na fitotoksičnost nanočestica i iona srebra u algi Chlorella vulgaris." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:486947

Đaković, Matea. "Učinak glukoze na fitotoksičnost nanočestica i iona srebra u algi Chlorella vulgaris." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:486947

Đaković, M. (2021). 'Učinak glukoze na fitotoksičnost nanočestica i iona srebra u algi Chlorella vulgaris', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:486947

Đaković M. Učinak glukoze na fitotoksičnost nanočestica i iona srebra u algi Chlorella vulgaris [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:486947

M. Đaković, "Učinak glukoze na fitotoksičnost nanočestica i iona srebra u algi Chlorella vulgaris", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:486947

Metadata
TitleUčinak glukoze na fitotoksičnost nanočestica i iona srebra u algi Chlorella vulgaris
Title (english)The effect of glucose on phytotoxicity of silver nanoparticles and ions in Chlorella vulgaris
AuthorMatea Đaković
MentorPetra Peharec Štefanić (mentor)
Committee memberPetra Peharec Štefanić (predsjednik povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberTatjana Bakran-Petricioli (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2021-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Nanočestice srebra (AgNP) koriste se u različitim granama industrije, ali zbog svoje toksičnosti i reaktivnosti predstavljaju opasnost za okoliš. S ciljem razumijevanja učinka glukoze na fitotoksičnost AgNP stabiliziranih polivinilpirolidonom (AgNP-PVP) i ionskog srebra (AgNO3) na algu Chlorella vulgaris, uzgajala sam alge na hranjivoj BBM podlozi s (Glc +) i bez dodatka glukoze (Glc -) i izlagala koncentracijama 0,5, 1,0, 1,5, 2,0 mg/L AgNP-PVP i AgNO3. Apsorpcijski spektar za AgNP-PVP pokazao je karakterističan pik za čestice srebra u oba tipa podloge, dok je apsorpcijski spektar za AgNO3 pokazao nastanak novih čestica srebra u oba tipa podloge nakon 1 sata. Sve ispitivane koncentracije AgNP-PVP i AgNO3 uzrokovale su smanjenje broja stanica i povećanje postotka neživih stanica u odnosu na kontrolu. Tretman s AgNP-PVP i AgNO3 uzrokovao je povećanje stupnja lipidne peroksidacije, te povećanje aktivnosti katalaze, superoksid dismutaze i peroksidaza u odnosu na kontrolu. Nakon oba tretmana uočene su značajne promjene u ultrastrukturi stanica algi u odnosu na kontrolu. Za sve izmjerene parametre, alge uzgojene na Glc + podlozi pokazale su znatno veću otpornost na oba tretmana i bolje preživljenje, iako je tretman s AgNO3 većinom bio toksičniji za alge.
Abstract (english)
Silver nanoparticles (AgNP) are used in various industries, but they pose a risk to the environment, due to their toxicity and reactivity. To understand the effect of glucose on the phytotoxicity of AgNP stabilized with polyvinylpyrrolidone (AgNP-PVP) and ionic silver (AgNO3) on Chlorella vulgaris, algae were grown on BBM nutrient medium with and without glucose and exposed to AgNP-PVP and AgNO3 at concentrations of 0.5, 1.0, 1, 5, 2.0 mg/L. The absorption spectra for AgNP-PVP had a characteristic peak for silver particles in both medium types, while the absorption spectra for AgNO3 showed that new silver particles were synthesised in both medium types after one hour. All tested concentrations of AgNP-PVP and AgNO3 led to a decrease in cell number and an increase in the percentage of dead cells relative to the control. Treatment with AgNP-PVP and AgNO3 caused an increase in lipid peroxidation, and in the activity of catalase, superoxide dismutase, and peroxidase relative to control. After both treatments, significant changes in the ultrastructure of cells were observed compared to the control. For all measured parameters, algae grown on a glucose-supplemented medium showed greater resistance and better survival to both treatments, while treatment with AgNO3 being more toxic to algae.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:486947
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-10-08 10:41:37