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master's thesis
Utjecaj utišavanja integrina alfavbeta3 i alfavbeta5 na osjetljivost stanica tumora na cisplatinu
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:578000
Dekanić, Ana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Dekanić, A. (2012). Utjecaj utišavanja integrina alfavbeta3 i alfavbeta5 na osjetljivost stanica tumora na cisplatinu (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:578000

Dekanić, Ana. "Utjecaj utišavanja integrina alfavbeta3 i alfavbeta5 na osjetljivost stanica tumora na cisplatinu." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:578000

Dekanić, Ana. "Utjecaj utišavanja integrina alfavbeta3 i alfavbeta5 na osjetljivost stanica tumora na cisplatinu." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:578000

Dekanić, A. (2012). 'Utjecaj utišavanja integrina alfavbeta3 i alfavbeta5 na osjetljivost stanica tumora na cisplatinu', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:578000

Dekanić A. Utjecaj utišavanja integrina alfavbeta3 i alfavbeta5 na osjetljivost stanica tumora na cisplatinu [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:578000

A. Dekanić, "Utjecaj utišavanja integrina alfavbeta3 i alfavbeta5 na osjetljivost stanica tumora na cisplatinu", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:578000

Metadata
TitleUtjecaj utišavanja integrina alfavbeta3 i alfavbeta5 na osjetljivost stanica tumora na cisplatinu
Title (english)Influence of integrin αvβ3 and αvβ5 silencing on sensitivity of tumor cells to cisplatin
AuthorAna Dekanić
MentorAndreja Ambriović Ristov (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberAndreja Ambriović Ristov (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Integrini su transmembranski proteini sastavljeni od α i β podjedinica koji sudjeluju u interakciji između stanica i izvanstaničnog matriksa. Integrini αvβ3 i αvβ5 su eksprimirani na endotelnim stanicama u angiogenezi i na stanicama mnogih tumora i stoga predstavljaju mete za terapiju. Osim toga, reguliraju osjetljivost stanica na protutumorske lijekove. U stanicama tumora dojke MDA-MB-435S istražili smo utjecaj utišavanja integrina αvβ3 i/ili αvβ5 uz pomoć siRNA specifičnih za β3, β5 i αv podjedinice. Pokazali smo da utišavanje β3 podjedinice snižava ekspresiju αvβ3, a povećava ekspresiju αvβ5 integrina, dok se utišavanjem β5 podjedinice snižava ekspresija integrina αvβ5 i povećava ekspresija αvβ3. Utišavanjem podjedinice αv smanjuje se ekspresija αvβ3 i αvβ5 integrina. Utišavanjem ekspresije αvβ3 i/ili αvβ5 integrina čini stanice manje osjetljivima na cisplatinu. Zaključujemo da bi kombinirani pristup liječenju tumora dojke koji eksprimiraju integrine αvβ3 i/ili αvβ5, αv, β3 ili β5-specifičnom siRNA i cisplatinom djelovao antagonistički.
Abstract (english)
Integrins are transmembrane proteins composed of α and β subunits, which actively participate in the interaction between cells and extracellular matrix. Integrins αvβ3 and αvβ5 are expressed on endothelial cells in angiogenesis and on many tumor cells, therefore they represent targets for tumor therapy. Further more, they regulate cell sensitivity to antitumor agents. In MDA-MB-435S breast cancer cell line we investigated the influence of integrin αvβ3 and/or αvβ5 silencing with siRNA specific for β3, β5 and αv subunits. We showed that silencing of β3 subunit decreases the expression of αvβ3, but increases the expression of αvβ5 integrin, while silencing of β5 subunit decreases the expression of αvβ5 and increases the expression of αvβ3. Silencing of αv subunit causes lowering of expression of both αvβ3 and αvβ5 integrins. Silencing of αvβ3 and/or αvβ5 integrin makes cells to be less sensitive to cisplatin. We conclude that the combined approach for αvβ3 and/or αvβ5 integrin positive breast cancer treatment, with siRNA specific for integrin subunits αv, β3 or β5 and cisplatin would have antagonistic effect.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:578000
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 12:40:59