prikaz prve stranice dokumenta Design of new nanostructured materials for application in biosensorics; theoretical study of optical properties of noble metal bio-nano hybrids
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doctoral thesis
Design of new nanostructured materials for application in biosensorics; theoretical study of optical properties of noble metal bio-nano hybrids
Split: University of Split, Faculty of Science, 2022. urn:nbn:hr:166:134502
Perić Bakulić, Martina
University of Split
Faculty of Science
Department of Physics

Institutional repository: Repository of Faculty of Science

Cite this document

Perić Bakulić, M. (2022). Design of new nanostructured materials for application in biosensorics; theoretical study of optical properties of noble metal bio-nano hybrids (Doctoral thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:134502

Perić Bakulić, Martina. "Design of new nanostructured materials for application in biosensorics; theoretical study of optical properties of noble metal bio-nano hybrids." Doctoral thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:134502

Perić Bakulić, Martina. "Design of new nanostructured materials for application in biosensorics; theoretical study of optical properties of noble metal bio-nano hybrids." Doctoral thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:134502

Perić Bakulić, M. (2022). 'Design of new nanostructured materials for application in biosensorics; theoretical study of optical properties of noble metal bio-nano hybrids', Doctoral thesis, University of Split, Faculty of Science, accessed 27 November 2024, https://urn.nsk.hr/urn:nbn:hr:166:134502

Perić Bakulić M. Design of new nanostructured materials for application in biosensorics; theoretical study of optical properties of noble metal bio-nano hybrids [Doctoral thesis]. Split: University of Split, Faculty of Science; 2022 [cited 2024 November 27] Available at: https://urn.nsk.hr/urn:nbn:hr:166:134502

M. Perić Bakulić, "Design of new nanostructured materials for application in biosensorics; theoretical study of optical properties of noble metal bio-nano hybrids", Doctoral thesis, University of Split, Faculty of Science, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:166:134502

Metadata
TitleDesign of new nanostructured materials for application in biosensorics; theoretical study of optical properties of noble metal bio-nano hybrids
Title (croatian)Dizajn novih nanostrukturnih materijala za primjenu u biosenzorici; teorijsko istraživanje optičkih svojstava bio-nano hibrida plemenitih metala
AuthorMartina Perić Bakulić
MentorVlasta Bonačić-Koutecky (mentor)
MentorMile Dželalija (komentor)
Committee memberAnte Bilušić (predsjednik povjerenstva)
Committee memberViljemka Bučević Popović (član povjerenstva)
Committee memberKatarina Vukojević (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Physics)
Split
Defense date and country2022-07-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
The focus of this doctoral thesis is to address the linear and nonlinear optical properties of noble metal nanoclusters (NCs) belonging to the nonscalable regime (∼ 2 nm). They can be embedded in the biological environment as bio-nano hybrids or protected with biomolecules as liganded NCs. Compared to standard bioimaging techniques (organic dyes and quantum dots) better sensitivity can be reached with the combination of two-photon spectroscopy and noble metal bio-nano systems. The research presented in this doctoral thesis is based on an investigation by computational chemistry methods. The combination of the QM/MM method with Density Functional Theory (DFT) and its time-dependent version (TD-DFT) allows the determination of structural and optical properties for bio-nano hybrids (silver trimer intercalated in DNA and silver doped gold-cysteine supramolecular assemblies) and liganded NCs (\(Au_{10}SG_{10}\), \(Au_{12}Zwitterions_{4}\), and \(Au_{15}SG_{13}\) NCs). Research in this doctoral thesis demonstrated that their unique optical properties (huge fluorescence for investigated bio-nano hybrids and bio-labeling capacity of liganded NCs) have the potential for applications in bioimaging and medical diagnostics. New routes of theoretical exploration are expected to lead the research towards biosensorics of superior sensitivity. Theoretical results will be compared with experimental results obtained by expert collaborators.
Abstract (croatian)
Težište ovog doktorskog rada počiva na linearnim i nelinearnim optičkim svojstvima nanoklastera plemenitih metala koji pripadaju neskalirajućim sustavima (∼ 2 nm). Mogu biti ugrađeni u biološki okoliš kao bio-nano hibridi ili zaštićeni biomolekulama kao ligandirani nanoklasteri. U usporedbi sa standardnim tehnikama bio-oslikavanja u medicinskoj dijagnostici (organske boje i kvantne točke) bolja osjetljivost može se postići kombinacijom dvofotonske spektroskopije i bio-nano sustava plemenitih metala. Istraživanje predstavljeno u ovom doktorskom radu temelji se na metodama računalne kvantne kemije. Kombinacija QM/MM metode s teorijom funkcionala gustoće (DFT) i njezinom vremenski ovisnom inačicom (TD-DFT) omogućuje određivanje strukturnih i optičkih svojstava za bio-nano hibride (trimer srebra interkaliran u DNK i supramolekularni sklopovi zlata i cisteina dopirani s jednim atomom srebra) te ligandirane nanoklastere (\(Au_{10}SG_{10}\), \(Au_{12}Zwitterions_{4}\), i \(Au_{15}SG_{13}\)). Istraživanje u ovom doktorskom radu pokazalo je da njihova jedinstvena optička svojstva (intezivna fluorescencija kod bio-nano hibrida te značajan kapacitet u bio-označavanju kod ligandiranih nanoklastera) imaju potencijal za primjenu u bio-oslikavanju i medicinskoj dijagnostici. Očekuje se da će novi pravci teorijskog istraživanja voditi istraživanja prema biosenzorici visoke osjetljivosti. Teorijski rezultati će se usporediti s eksperimentalnim rezultatima dobivenim od strane eksperimentalnih stručnjaka
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:166:134502
Study programmeTitle: Postgraduate University Study of Biophysics
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje fizika (doktor/doktorica znanosti, područje prirodnih znanosti, polje fizika)
Type of resourceText
Extent122 str.; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2022-07-29 11:18:11