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master's thesis
Određivanje rezistencije na klaritromicin i levofloksacin metodom PCR u bakterije Helicobacter pylori izolirane iz sluznice želuca
Split: University of Split, University of Split, Faculty of science, 2022. urn:nbn:hr:166:469824
Dadić, Blanka
University of Split
Faculty of Science
Department of Biology

Institutional repository: Repository of Faculty of Science

Cite this document

Dadić, B. (2022). Određivanje rezistencije na klaritromicin i levofloksacin metodom PCR u bakterije Helicobacter pylori izolirane iz sluznice želuca (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:469824

Dadić, Blanka. "Određivanje rezistencije na klaritromicin i levofloksacin metodom PCR u bakterije Helicobacter pylori izolirane iz sluznice želuca." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:469824

Dadić, Blanka. "Određivanje rezistencije na klaritromicin i levofloksacin metodom PCR u bakterije Helicobacter pylori izolirane iz sluznice želuca." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:469824

Dadić, B. (2022). 'Određivanje rezistencije na klaritromicin i levofloksacin metodom PCR u bakterije Helicobacter pylori izolirane iz sluznice želuca', Master's thesis, University of Split, Faculty of Science, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:166:469824

Dadić B. Određivanje rezistencije na klaritromicin i levofloksacin metodom PCR u bakterije Helicobacter pylori izolirane iz sluznice želuca [Master's thesis]. Split: University of Split, Faculty of Science; 2022 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:166:469824

B. Dadić, "Određivanje rezistencije na klaritromicin i levofloksacin metodom PCR u bakterije Helicobacter pylori izolirane iz sluznice želuca", Master's thesis, University of Split, Faculty of Science, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:166:469824

Metadata
TitleOdređivanje rezistencije na klaritromicin i levofloksacin metodom PCR u bakterije Helicobacter pylori izolirane iz sluznice želuca
Title (english)Detection of clarithromycin and levofloxacin resistance by the PCR method in Helicobacter pylori from gastric biopsy specimens
AuthorBlanka Dadić
MentorIvica Šamanić (mentor)
Committee memberIvica Šamanić (predsjednik povjerenstva)
Committee memberAna Maravić (član povjerenstva)
Committee memberMia Dželalija (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Biology)
Split
Defense date and country2022-09-15, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Helicobacter pylori je patogena bakterija koja je uzročnik gastrointestinalnih bolesti poput gastritisa, peptičkog ulkusa i raka želuca kod čovjeka. Tijekom posljednja dva desetljeća zabilježen je značajan porast rezistencije H. pylori na mnoge antibiotike uključujući klaritromicin i levofloksacin. Uzrok rezistencije klaritromicina i levofloksacina su točkaste mutacije u genetičkoj sekvenci koje ometaju staničnu aktivnost antibiotika mijenjajući ciljno mjesto lijeka. Cilj diplomskog rada bila je molekularna karakterizacija ili analiza sekvenci triju gena čije mutacije su povezane sa rezistencijom na antibiotike klaritromicin i levofloksacin. U ovom istraživanju identificiran je niz mutacija u genu 23s rRNA odgovornih za rezistenciju na klaritromicin te mutacije u podjedinici A DNA giraze koje su povezane sa rezistencijom na levofloksacin. Nisu detektirane mutacije u podjedinici B DNA giraze ključne za rezistenciju na levofloksacin. Isto tako uočena je višestruka rezistencija što ukazuje na uspješan razvoj otpornosti na različite antibiotike i posljedično povećani rizik za ljudsko zdravlje.
Abstract (english)
Helicobacter pylori is a pathogenic bacterium that causes gastrointestinal diseases such as gastritis, peptic ulcer and stomach cancer in humans. Over the past two decades there has been a significant increase in H. pylori resistance to many antibiotics including clarithromycin and levofloxacin. The cause of resistance of clarithromycin and levofloxacin are point mutations in the genetic sequence that disrupt the cellular activity of antibiotics by altering the drug target site. The aim of the thesis was the molecular characterization or sequence analysis of three genes whose mutations are associated with resistance to the clarithromycin and levofloxacin. In this study a series of mutations in the 23s rRNA gene responsible for resistance to clarithromycin and mutations in DNA gyrase subunit A which are associated with resistance to levofloxacin were identified. No mutations in DNA gyrase subunit B crucial for levofloxacin resistance were detected. Multidrug resistance was also observed which indicates the successful development of resistance to different antibiotics and consequently an increased risk to human health.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:469824
Study programmeTitle: Biology and Chemistry; specializations in: major in teaching
Course: major in teaching
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-11-02 13:59:58