prikaz prve stranice dokumenta Electroformation of giant unilamellar vesicles from uniform lipid film
  Download
PDF 2.6 MB
master's thesis
Electroformation of giant unilamellar vesicles from uniform lipid film
Split: University of Split, Faculty of Science, 2022. urn:nbn:hr:166:678766
Šumanovac, Josipa
University of Split
Faculty of Science
Department of Physics

Institutional repository: Repository of Faculty of Science

Cite this document

Šumanovac, J. (2022). Electroformation of giant unilamellar vesicles from uniform lipid film (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:678766

Šumanovac, Josipa. "Electroformation of giant unilamellar vesicles from uniform lipid film." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:678766

Šumanovac, Josipa. "Electroformation of giant unilamellar vesicles from uniform lipid film." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:678766

Šumanovac, J. (2022). 'Electroformation of giant unilamellar vesicles from uniform lipid film', Master's thesis, University of Split, Faculty of Science, accessed 11 July 2024, https://urn.nsk.hr/urn:nbn:hr:166:678766

Šumanovac J. Electroformation of giant unilamellar vesicles from uniform lipid film [Master's thesis]. Split: University of Split, Faculty of Science; 2022 [cited 2024 July 11] Available at: https://urn.nsk.hr/urn:nbn:hr:166:678766

J. Šumanovac, "Electroformation of giant unilamellar vesicles from uniform lipid film", Master's thesis, University of Split, Faculty of Science, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:166:678766

Metadata
TitleElectroformation of giant unilamellar vesicles from uniform lipid film
Title (croatian)Elektroformacija gigantskih jednoslojnih vezikula sa homogenog lipidnog filma
AuthorJosipa Šumanovac
MentorMarija Raguž (mentor)
Committee memberMarija Raguž (predsjednik povjerenstva)
Committee memberLarisa Zoranić (član povjerenstva)
Committee memberAnte Bilušić (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Physics)
Split
Defense date and country2022-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Biophysics and Medical Physics
Abstract
The plasma membrane (PM) is a structure that separates intercellular and extracellular fluid. It is composed of lipid bilayers and membrane proteins. Membrane properties and interaction with different molecules are often studied using laboratory-made membrane models under controlled conditions. One of the commonly used membrane models is Giant Unillamilar Vesicles (GUVs). In this thesis, two methods of film deposition of large unillamilar vesicles (LUVs) prior to electroforming of GUVs are tested: Spin-Coating Method and Coffee Ring Effect Method. GUVs containing Chol/POPC (Cholesterol/ Phosphatidylcholine) with different Chol ratios (from 0 to 3) are prepared. We quantify electroformation successfulness using fluorescence microscopy and concluded that the coffee ring effect is not an optimal method for film deposition and that a concentration of 3 mg/ml LUVs is optimal for the spin-coating method.
Abstract (croatian)
Plazma membrane (PM) su strukture koje odvajaju unutarstaničnu od izvanstanične tekućine. Sastavljene su od lipidnog dvosloja i membranskih proteina. Svojstva membrane te njezina interakcija sa drugim molekulama i lijekovima istražuju se laboratorijski dobivenim membranskim modelima uz kontrolirane uvjetima. Najčešće korišteni modeli su gigantske jednoslojne vezikule (GUV-ovi). U ovom diplomskom radu, prije elektroformacije GUV-ova korištene su dvije metode za nanošenja filma od gigantskih jednoslojnih vezikula (LUV-ova): Spin-Coating metoda i Coffee Ring metoda. Pripremljeni su GUV-ovi od kolesterola i fosfatidilkolina (Chol/POPC) sa različitim udjelom kolesterola (od 0 do 3). Uspješnost elektroformacije provjeravamo korištenjem fluorescentne mikroskopije i zaključujemo da Coffee Ring metoda nije optimalna za nanošenje filma te da je koncentracija LUV-ova od 3 mg/mL optimalna za Spin-Coating metodu.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:166:678766
Study programmeTitle: Physics; specializations in: major in teaching, major in Astrophysics, major in Biophysics, major in Enviromental physics, major in Computational physics, major in Astrophysics and physics of elemental particles
Course: major in Biophysics
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra fizike (magistar/magistra fizike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-04-07
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-02-15 09:08:00