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master's thesis
Citogenomska analiza minisatelitne ponavljajuće sekvence Sat97 u rodu Allium, sekcije Cepa
Split: University of Split, Faculty of Science, 2022. urn:nbn:hr:166:157388
Budimir, Tina
University of Split
Faculty of Science
Department of Biology

Institutional repository: Repository of Faculty of Science

Cite this document

Budimir, T. (2022). Citogenomska analiza minisatelitne ponavljajuće sekvence Sat97 u rodu Allium, sekcije Cepa (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:157388

Budimir, Tina. "Citogenomska analiza minisatelitne ponavljajuće sekvence Sat97 u rodu Allium, sekcije Cepa." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:157388

Budimir, Tina. "Citogenomska analiza minisatelitne ponavljajuće sekvence Sat97 u rodu Allium, sekcije Cepa." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:157388

Budimir, T. (2022). 'Citogenomska analiza minisatelitne ponavljajuće sekvence Sat97 u rodu Allium, sekcije Cepa', Master's thesis, University of Split, Faculty of Science, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:166:157388

Budimir T. Citogenomska analiza minisatelitne ponavljajuće sekvence Sat97 u rodu Allium, sekcije Cepa [Master's thesis]. Split: University of Split, Faculty of Science; 2022 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:166:157388

T. Budimir, "Citogenomska analiza minisatelitne ponavljajuće sekvence Sat97 u rodu Allium, sekcije Cepa", Master's thesis, University of Split, Faculty of Science, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:166:157388

Metadata
TitleCitogenomska analiza minisatelitne ponavljajuće sekvence Sat97 u rodu Allium, sekcije Cepa
Title (english)Cytogenomic analysis of a Sat97 minisatellite repeat sequence in the genus Allium, section Cepa
AuthorTina Budimir
MentorIvica Šamanić (mentor)
Committee memberIvica Šamanić (predsjednik povjerenstva)
Committee memberJasna Puizina (član povjerenstva)
Committee memberŽeljana Fredotović (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Biology)
Split
Defense date and country2022-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Rekonstrukcija podrijetla i evolucije poliploidnih genoma od divljih srodnika vrlo je značajna u citogenetičkim istraživanjima. Satelitske sekvence DNA koje tvore dugačke nizove uzastopno ponavljajućih jedinica prisutne su u svim biljnim genomima, ali mogu varirati u nizu značajki, uključujući sastav nukleotida, brojnost i distribuciju u genomima. Stoga, satelitske DNA brzo divergiraju i često su specifične za vrstu ili rod. Rekonstrukcija jednog tipa satelitne porodice na temelju rezultata sekvenciranja sljedeće generacije (NGS) u kombinaciji s bioinformatičkom platformom Repeat Explorer i algoritmom TAREAN, pridonijela je našem boljem razumijevanju složenog porijekla alotriploidnog luka Allium × cornutum. Primjena fluorescencijske hibridizacije in situ (FISH) pomoću fluorescentno obilježenih oligonukleotidnih sondi omogućuje odgovore na pitanja vezana uz strukturu, mutacije i evoluciju ne samo pojedinačnih kromosoma već i cijelih genoma. U ovom radu, koristeći podatke NGS sekvenciranja identificirali smo ponavljajuću satelitnu sekvencu veličine 97 bp (AceSat97) i mapirali njenu distribuciju na kromosomima alotriploidnog luka A. × cornutum i tri pretpostavljene roditeljske diploidne vrste Allium cepa, A. pskemense i A. roylei.
Abstract (english)
Reconstructing the origin and evolution of polyploid genomes from wild relatives is very important for cytogenetic research. Satellite DNA sequences, which form long arrays of tandemly repeated units, are present in all plant genomes but can vary in a number of features, including nucleotide composition, abundance, and genome distribution. Therefore, satellite DNAs diverge rapidly and are often species- or genus-specific. Reconstruction of a satellite family species based on the results of next-generation sequencing (NGS) combined with the Repeat Explorer bioinformatics platform and the TAREAN algorithm has contributed to a better understanding of the complex origin of the allotriploid onion Allium × cornutum. The application of fluorescence in situ hybridization (FISH) using fluorescently labeled oligonucleotide probes allows us to answer questions about the structure, mutations, and evolution of not only single chromosomes but entire genomes. In this work, using NGS sequencing data, we identified a repetitive satellite sequence of size 97 bp (AceSat97) and mapped its distribution on the chromosomes of the allotriploid onion A. × cornutum and the three putative diploid parental species Allium cepa, A. pskemense, and A. roylei.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:157388
ProjectNumber: IP-2016-06-8432
Title: Genomske i epigenomske promjene u auto- i alopoliploidima na modelu dalmatinskog buhača, ljutike i anemona
Title: Genomic and epigenomic changes in auto- and allopolypids: case studies on dalmatian pyrethrum, shallot and anemone
Acronym: AutoAloPol
Leader: Višnja Besendorfer
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Biology and Chemistry; specializations in: major in teaching
Course: major in teaching
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to authenticated users
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Created on2023-02-15 10:17:56