prikaz prve stranice dokumenta Inhibitorni učinak hidrolata i eteričnih ulja odabranih vrsta roda Veronica na infekciju virusom mozaika duhana
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master's thesis
Inhibitorni učinak hidrolata i eteričnih ulja odabranih vrsta roda Veronica na infekciju virusom mozaika duhana
Split: University of Split, Faculty of Science, 2022. urn:nbn:hr:166:299116
Mandić, Mihaela
University of Split
Faculty of Science
Department of Biology

Institutional repository: Repository of Faculty of Science

Cite this document

Mandić, M. (2022). Inhibitorni učinak hidrolata i eteričnih ulja odabranih vrsta roda Veronica na infekciju virusom mozaika duhana (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:299116

Mandić, Mihaela. "Inhibitorni učinak hidrolata i eteričnih ulja odabranih vrsta roda Veronica na infekciju virusom mozaika duhana." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:299116

Mandić, Mihaela. "Inhibitorni učinak hidrolata i eteričnih ulja odabranih vrsta roda Veronica na infekciju virusom mozaika duhana." Master's thesis, University of Split, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:166:299116

Mandić, M. (2022). 'Inhibitorni učinak hidrolata i eteričnih ulja odabranih vrsta roda Veronica na infekciju virusom mozaika duhana', Master's thesis, University of Split, Faculty of Science, accessed 02 October 2024, https://urn.nsk.hr/urn:nbn:hr:166:299116

Mandić M. Inhibitorni učinak hidrolata i eteričnih ulja odabranih vrsta roda Veronica na infekciju virusom mozaika duhana [Master's thesis]. Split: University of Split, Faculty of Science; 2022 [cited 2024 October 02] Available at: https://urn.nsk.hr/urn:nbn:hr:166:299116

M. Mandić, "Inhibitorni učinak hidrolata i eteričnih ulja odabranih vrsta roda Veronica na infekciju virusom mozaika duhana", Master's thesis, University of Split, Faculty of Science, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:166:299116

Metadata
TitleInhibitorni učinak hidrolata i eteričnih ulja odabranih vrsta roda Veronica na infekciju virusom mozaika duhana
Title (english)Inhibitory activity of hydrosols and essential oils of selected species of the genus Veronica on tobacco mosaic virus infection
AuthorMihaela Mandić
MentorElma Vuko (mentor)
Committee memberElma Vuko (predsjednik povjerenstva)
Committee memberIvana Bočina (član povjerenstva)
Committee memberValerija Dunkić (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Biology)
Split
Defense date and country2022-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Rod Veronica broji oko 500 vrsta zeljastih jednogodišnjih biljaka ili trajnica iz porodice Plantaginaceae. Vrste ovog roda karakterizira varijabilnost morfologije, životnih oblika i staništa. U ovom istraživanju korišteno je jedanaest vrsta roda Veronica od kojih su, vodenom destilacijom i mikrovalnom ekstrakcijom, pripremljeni eterična ulja i hidrolati te je ispitana njihova protuvirusna aktivnost na domaćinskim biljkama inficiranima virusom mozaika duhana (tobacco mosiac virus, TMV). Predtretman biljaka hidrolatom i simultana inokulacija eteričnog ulja i virusa inhibirali su razvoj lokalnih virusnih lezija u usporedbi sa kontrolnim biljkama, pri čemu je protuvirusni učinak hidrolata jače izražen u odnosu na učinak eteričnih ulja. Hidrolati i eterična ulja dobiveni vodenom destilacijom imaju bolji učinak na inhibiciju virusne infekcije od ekstrakata dobivenih mikrovalnom ekstrakcijom. Citopatološke promjene u inficiranim stanicama zabilježene su elektronskom mikroskopijom sistemično inficiranih listova duhana. Kao potpora ekološkoj proizvodnji i zamjeni sintetičkih kemikalija tvarima prirodnog porijekla, nužna je potraga za novim prirodnim bioaktivnim supstancama. Rasprostranjenost vrsta roda Veronica i dobiveni rezultati ukazuju na značajan potencijal vrsta ovog roda za razvoj novih, prirodnih protufitovirusnih sredstava.
Abstract (english)
The genus Veronica includes about 500 species of annual or perennial plants in the family Plantaginaceae. The species of this genus are characterized by variability in morphology, life forms and habitats. In this study, 11 species of the genus Veronica were used, from which essential oils and hydrolates were prepared by hydrodistillation and microwave extraction. Their antiviral activity was tested on host plants infected with tobacco mosaic virus (TMV). Pretreatment of plants with hydrolate and simultaneous inoculation of essential oil and virus showed an inhibitory effect on the development of local viral lesions compared to control plants, and the antiviral effect of hydrolate was slightly stronger than the effect of essential oils. Hydrolates and essential oils obtained by hydrodistillation have a better effect on the inhibition of viral infection than the same extracts obtained by microwave extraction. Cytopathological changes in infected cells were detected by electron microscopy of systemically infected leaves. To support organic production and to replace synthetic chemicals with substances of natural origin, the search for new natural bioactive substances is necessary. The distribution of the species of the genus Veronica and the results obtained indicate a significant potential for the use of the species of this genus in the development of new, natural antiphytoviral agents.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:299116
ProjectNumber: IP-2020-02-8425
Title: Hrvatske vrste roda Veronica: fitotaksonomija i biološka aktivnost
Title: Croatian Veronica species: Phytotaxonomy and Biological Activity
Acronym: CROVeS-PhyBA
Leader: Valerija Dunkić
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Biology and Chemistry; specializations in: major in teaching
Course: major in teaching
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2023-02-16 13:15:02