prikaz prve stranice dokumenta Aktivnost antimikrobnih peptida iz trakavice (Taenia solium Linnaeus, 1758) naspram gram-negativnih bakterija i karcinomskih staničnih linija
Embargo period 2025-12-11
master's thesis
Aktivnost antimikrobnih peptida iz trakavice (Taenia solium Linnaeus, 1758) naspram gram-negativnih bakterija i karcinomskih staničnih linija
Split: University of Split, Faculty of Science, 2024. urn:nbn:hr:166:705996
Sabljić, Angela
University of Split
Faculty of Science
Department of Biology

Institutional repository: Repository of Faculty of Science

Cite this document

Sabljić, A. (2024). Aktivnost antimikrobnih peptida iz trakavice (Taenia solium Linnaeus, 1758) naspram gram-negativnih bakterija i karcinomskih staničnih linija (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:705996

Sabljić, Angela. "Aktivnost antimikrobnih peptida iz trakavice (Taenia solium Linnaeus, 1758) naspram gram-negativnih bakterija i karcinomskih staničnih linija." Master's thesis, University of Split, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:166:705996

Sabljić, Angela. "Aktivnost antimikrobnih peptida iz trakavice (Taenia solium Linnaeus, 1758) naspram gram-negativnih bakterija i karcinomskih staničnih linija." Master's thesis, University of Split, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:166:705996

Sabljić, A. (2024). 'Aktivnost antimikrobnih peptida iz trakavice (Taenia solium Linnaeus, 1758) naspram gram-negativnih bakterija i karcinomskih staničnih linija', Master's thesis, University of Split, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:166:705996

Sabljić A. Aktivnost antimikrobnih peptida iz trakavice (Taenia solium Linnaeus, 1758) naspram gram-negativnih bakterija i karcinomskih staničnih linija [Master's thesis]. Split: University of Split, Faculty of Science; 2024 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:166:705996

A. Sabljić, "Aktivnost antimikrobnih peptida iz trakavice (Taenia solium Linnaeus, 1758) naspram gram-negativnih bakterija i karcinomskih staničnih linija", Master's thesis, University of Split, Faculty of Science, Split, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:166:705996

Metadata
TitleAktivnost antimikrobnih peptida iz trakavice (Taenia solium Linnaeus, 1758) naspram gram-negativnih bakterija i karcinomskih staničnih linija
Title (english)Activity of antimicrobal peptides from tapeworm (Taenia solium Linnaeus, 1758) against gram-negative bacteria and cancer cell lines
AuthorAngela Sabljić
MentorTomislav Rončević (mentor)
Committee memberTomislav Rončević (predsjednik povjerenstva)
Committee memberŽeljana Fredotović (član povjerenstva)
Committee memberIvica Šamanić (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Biology)
Split
Defense date and country2024-04-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Globalno širenje antimikrobne rezistencije jedna je od vodećih prijetnji suvremenog doba. Sposobnost brze prilagode bakterija na okolišne čimbenike sve češće rezultira pojavom rezistentih bakterija dovodeći tako u pitanje učinkovitost postojećih antibiotika. Izazov današnjice je pronalazak bioaktivnih tvari efektivnih u suzbijanju bakterijskih infekcija, a potencijalni obećavajući kandidati su antimikrobni peptidi (AMP) - multifunkcionalne efektorske molekule s direktnom antimikrobnom aktivnosti i/ili imunomodulatornim djelovanjem. Pozornost diplomskog rada usmjerena je na karakterizaciju i proučavanje širokog spektra djelovanja peptida identificiranog u trakavici Taenia solium (Linnaeus, 1758), nazvanog TSO8, kao i njegovih fragmenata. U tom kontekstu, spektroskopijom cirkularnog dikroizma opažene su strukturne promjene TSO8 peptida u izotropnom i anizotropnim otapalu, te u prisutnosti liposoma. Ispitana je antimikrobna aktivnost svih peptida na panelu gram-negativnih bakterija, te se nastojalo djelomično definirati mehanizam djelovanja TSO8 peptida promatrajući kinetiku rasta bakterija u ovisnosti o različitim koncentracijama peptida. S druge strane, makromolekule ovog tipa često imaju i antitumorsko djelovanje koje je ispitano MTT testom na karcinomskoj staničnoj liniji mokraćnog mjehura i karcinomskoj liniji raka dojke.
Abstract (english)
The rapid spread of antimicrobial resistance is one of the leading threats of modern times. The ability of bacteria to quickly adapt to environmental factors is rapidly resulting in the emergence of resistant bacterial strains, thus calling into question the effectiveness of existing antibiotics. Today's challenge is to find bioactive substances effective in suppressing bacterial infections, and potential lead compounds in this sense are antimicrobial peptides (AMPs) - multifunctional effector molecules with direct antimicrobial activity and/or immunomodulatory properties. The focus of the thesis is on the characterization and study of the broad spectrum of action of the peptide identified in the tapeworm Taenia solium (Linnaeus, 1758), and therefore named TSO8, as well as its fragments. In this context, structural changes of TSO8 peptides in isotropic and anisotropic solvent and in the presence of liposomes were observed by circular dichroism spectroscopy. The antimicrobial activity of all peptides was tested against a panel of Gram-negative bacteria, and an attempt was made to partially define the mechanism of action of the these AMPs by observing the kinetics of bacterial growth depending on different peptide concentrations. On the other hand, macromolecules of this type often also have anticancer properties, which was tested by the MTT test on a bladder cancer and a breast cancer cell line.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:705996
Study programmeTitle: Biology and Chemistry; specializations in: specialization in Education
Course: specialization in Education
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra edukacije biologije i kemije (sveučilišni/a magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-12-11
Terms of useLicence In copyright icon
Created on2024-05-06 09:20:08