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master's thesis
Kvantitativna analiza duljina telomera starih i mladih donora u primarnim stanicama fibroblasta iz kože metodom hibridizacije in situ, Q-PNA-FISH
Split: University of Split, University of Split, Faculty of science, 2017. urn:nbn:hr:166:710388
Pavlinović, Mia
University of Split
Faculty of Science
Department of Biology

Institutional repository: Repository of Faculty of Science

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Pavlinović, M. (2017). Kvantitativna analiza duljina telomera starih i mladih donora u primarnim stanicama fibroblasta iz kože metodom hibridizacije in situ, Q-PNA-FISH (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:710388

Pavlinović, Mia. "Kvantitativna analiza duljina telomera starih i mladih donora u primarnim stanicama fibroblasta iz kože metodom hibridizacije in situ, Q-PNA-FISH." Master's thesis, University of Split, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:166:710388

Pavlinović, Mia. "Kvantitativna analiza duljina telomera starih i mladih donora u primarnim stanicama fibroblasta iz kože metodom hibridizacije in situ, Q-PNA-FISH." Master's thesis, University of Split, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:166:710388

Pavlinović, M. (2017). 'Kvantitativna analiza duljina telomera starih i mladih donora u primarnim stanicama fibroblasta iz kože metodom hibridizacije in situ, Q-PNA-FISH', Master's thesis, University of Split, Faculty of Science, accessed 17 July 2024, https://urn.nsk.hr/urn:nbn:hr:166:710388

Pavlinović M. Kvantitativna analiza duljina telomera starih i mladih donora u primarnim stanicama fibroblasta iz kože metodom hibridizacije in situ, Q-PNA-FISH [Master's thesis]. Split: University of Split, Faculty of Science; 2017 [cited 2024 July 17] Available at: https://urn.nsk.hr/urn:nbn:hr:166:710388

M. Pavlinović, "Kvantitativna analiza duljina telomera starih i mladih donora u primarnim stanicama fibroblasta iz kože metodom hibridizacije in situ, Q-PNA-FISH", Master's thesis, University of Split, Faculty of Science, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:166:710388

Metadata
TitleKvantitativna analiza duljina telomera starih i mladih donora u primarnim stanicama fibroblasta iz kože metodom hibridizacije in situ, Q-PNA-FISH
Title (english)Telomere lenght analysis by quantitative fluorescent in situ hybridization, Q-PNA-FISH, in primary skin fibroblasts from young and adult donors
AuthorMia Pavlinović
MentorIvica Šamanić (mentor)
Committee memberIvica Šamanić (predsjednik povjerenstva)
Committee memberJasna Puizina (član povjerenstva)
Committee memberŽeljana Fredotović (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Biology)
Split
Defense date and country2017-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Struktura i funkcija telomera bitne su za razumijevanje procesa staničnog starenja. Produžavanje telomera glavni je mehanizam održavanja besmrtnosti tumorskih stanica. U ovom radu proučena je dinamika telomera u kulturi ljudskih fibroblasta iz kože mladih i starih donora. Primarne stanice fibroblasta tretirane su sredstvom za pomlađivanje p19 kako bi se utvrdio mogući utjecaj na duljinu telomera. Telomere stanica fibroblasta iz kože kvantitativno su analizirane metodom hibridizacije in situ, Q-PNA-FISH. Cilj rada je optimiziranje metode za analizu intenziteta telomernih signala dobivenih putem Q-PNA-FISH tehnike, te uspostavljanje statističke metode za kvantificiranje dobivenih vrijednosti.
Abstract (english)
Telomere structure and function are essential for understanding cellular aging process. The extension of telomere repeats represents a major mechanism for maintaining the immortality of many tumor cells. In this paper telomere length in human skin fibroblasts from young and old donor has been studied. Primary human fibroblasts were treated with p19 rejuvenating agent to determine its ability to fight off aging process.Telomere lengths in the primary cultured human skin fibroblasts were analyzed using quantitative fluorescence in situ hybridization, Q-PNA-FISH. The aim of this thesis is to optimise Q-PNA-FISH telomere signal intensity analysis method and to establish a statistical method to quantify the fluorescent signal obtained from a telomere-specific probe.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:166:710388
Study programmeTitle: Biology and Chemistry; specializations in: major in teaching
Course: major in teaching
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2019-02-12
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-02-21 17:05:09